Vollautomatische Auswertung von Dünnschicht-Chromatogrammen 1. Mitteilung: Analog/Zeit — Scanautomatik

Ebel, S.; Herold, G.; Hocke, J.

doi:10.1007/bf02314043

Cited by 14 publications

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“…the internal standard method will give more reliable results than the external standard method. Thiscondition is fulfilled in computer controlled TLC and HPTLC [13,14] and in most cases using electronic integrators [15], if the chromatographic process has been optimized.…”

Section: Comparison Of Both Methodsmentioning

confidence: 99%

Systematic and statistical errors in quantitative evaluation using TLC and HPTLC. 2. Internal and external standard methods

Ebel

Glaser

Rost

1978

J. High Resol. Chromatogr.

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Key Words:Thin Layer Chromatography, TLC, HPTLC Quantitative evaluation Errors, systematic and statistical Error propagation for routine analysis with internal or external standards SummaryThe error propagation is given for routine analysis by in situ evaluation in TLC using the methods with internal or external standard. The internal standard method will need four different measurements of peak height or area, while the external standard method uses only two measurements. Therefore in the latter case the error of the spotting volume will cause errors in the determined concentration. By error propagation it can be shown, that the internal standard method gives better results, if the error in measuring peak height or area is less than the error of the spotting volume.

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Section: Comparison Of Both Methodsmentioning

confidence: 99%