Combined measurements of water isotopologues of a snow pit at Vostok over the past 60 y reveal a unique signature that cannot be explained only by climatic features as usually done. Comparisons of the data using a general circulation model and a simpler isotopic distillation model reveal a stratospheric signature in the 17 O-excess record at Vostok. Our data and theoretical considerations indicate that mass-independent fractionation imprints the isotopic signature of stratospheric water vapor, which may allow for a distinction between stratospheric and tropospheric influences at remote East Antarctic sites.S table water isotopic ratios (δ 18 O and δ 2 H) have been used for many years as a proxy for local temperature T s reconstruction in East Antarctica (1, 2). The link with temperature results from mass-dependent isotopic fractionation of water at each phase transition along the water mass trajectory from the evaporative zone to the polar precipitation site. Two different kinds of massdependent fractionation effects lead to the depletion in heavy isotopologues of the water vapor. First, equilibrium fractionation is caused by the lower vapor pressure for the heavy isotopologues compared with the one for the abundant light (H 2 O) water molecules. Second, kinetic fractionation leads to isotopic fractionation due to different molecular diffusivity constants of the light and heavy water isotopologues (light molecules diffuse faster in air than the heavy ones) (3). The observed spatial slope of Δδ 18 O vs. ΔT s lies between 0.75‰ and 0.8‰°C −1 [±20% at glacial timescales (4-6)] and builds the basis for past temperature reconstruction from δ 18 O in ice cores. It should be noted that this relationship can be associated with larger uncertainties of factor 2 for warmer than present-day climates (7). Biases to a constant temporal δ 18 O vs. temperature slope may arise from changes of moisture origin for the polar precipitation, precipitation intermittency at the seasonal or interannual scale, postdeposition effects, and changes of moisture trajectories. Tools exist to quantify such biases. First, the second-order parameters deuterium (d)-excess (8) ·y −1 water equivalent). In addition, Vostok is located within the Antarctic vortex, which makes it sensitive to stratospheric input (up to 5%) (12). Only onequarter of the precipitation originates from tropospheric snowfall, whereas 75% is due to hoar frost deposition and ice needle fallout (diamond dust), which may originate from the stratosphere (13,14). The influence of such stratospheric water vapor input has been only marginally investigated even if the existence of mass independent fractionation (MIF) in the stratosphere is expected to strongly affect 17 O-excess (15). This is because other effects become also prominent in these regions. First, at the observed very low temperature range (<−50°C), the secondorder parameters d-excess and 17 O-excess are expected to show strong variations with condensation temperature (16). Second, the interpretation of water isotopic pr...