Volatile Organic Metabolites from Selected Streptomyces Strains

Wilkins, Ken; Schöller, Charlotte

doi:10.3209/saj.saj230202

Cited by 44 publications

(43 citation statements)

References 15 publications

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“…For example, quorum signals and siderophores produced by one organism can be taken up or used by others (Lyons and Kolter, 2015; Traxler et al, 2012). The VOC repertoire of microorganisms appears to be vast (Chuankun et al, 2004; Insam and Seewald, 2010; Kai et al, 2009; Schöller et al, 2002; Schulz and Dickschat, 2007; Wilkins and Schöller, 2009). Volatile compounds have historically been implicated in the ‘avoidance responses’ of fungi, promoting their growth away from inanimate objects (Cohen et al, 1975; Gamow and Böttger, 1982).…”

Section: Discussionmentioning

confidence: 99%

Streptomyces exploration is triggered by fungal interactions and volatile signals

Jones

Rees

et al. 2017

eLife

160

145

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It has long been thought that the life cycle of Streptomyces bacteria encompasses three developmental stages: vegetative hyphae, aerial hyphae and spores. Here, we show interactions between Streptomyces and fungi trigger a previously unobserved mode of Streptomyces development. We term these Streptomyces cells ‘explorers’, for their ability to adopt a non-branching vegetative hyphal conformation and rapidly transverse solid surfaces. Fungi trigger Streptomyces exploratory growth in part by altering the composition of the growth medium, and Streptomyces explorer cells can communicate this exploratory behaviour to other physically separated streptomycetes using an airborne volatile organic compound (VOC). These results reveal that interkingdom interactions can trigger novel developmental behaviours in bacteria, here, causing Streptomyces to deviate from its classically-defined life cycle. Furthermore, this work provides evidence that VOCs can act as long-range communication signals capable of propagating microbial morphological switches.DOI: http://dx.doi.org/10.7554/eLife.21738.001

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Section: Discussionmentioning

confidence: 99%

Streptomyces exploration is triggered by fungal interactions and volatile signals

Jones

Rees

et al. 2017

eLife

160

145

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“…A wide variety of compounds occurring in M. tuberculosis strains may serve as basis for the odor detection. We identified volatiles shared by M. tuberculosis, Nocardia spp., Streptomyces spp., and Rhodococcus sp., for example, 2-phenylethanol or 2-hydroxy-3-pentanone (Table 3), which are also produced by other microbial species (28,29,37,41) and hence cannot be regarded as specific markers for M. tuberculosis. For example, aciphyllene, which is a known sesquiterpene from the endophytic fungus Muscodor albus (3), is a typical volatile compound of Nocardia spp.…”

Section: Resultsmentioning

confidence: 99%

Diagnosis of Tuberculosis by Trained African Giant Pouched Rats and Confounding Impact of Pathogens and Microflora of the Respiratory Tract

Mgode

Weetjens²,

Nawrath

et al. 2012

J Clin Microbiol

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Trained African giant-pouched rats (Cricetomys gambianus) can detect Mycobacterium tuberculosis and show potential for the diagnosis of tuberculosis (TB). However, rats' ability to discriminate between clinical sputum containing other Mycobacterium spp. and nonmycobacterial species of the respiratory tract is unknown. It is also unknown whether nonmycobacterial species produce odor similar to M. tuberculosis and thereby cause the detection of smear-negative sputum. Sputum samples from 289 subjects were analyzed by smear microscopy, culture, and rats. Mycobacterium spp. were isolated on Lowenstein-Jensen medium, and nonmycobacterial species were isolated on four different media. The odor from nonmycobacterial species from smear-and M. tuberculosis culture-negative sputa detected by >2 rats ("rat positive") was analyzed by gas chromatographymass spectrometry and compared to the M. tuberculosis odor. Rats detected 45 of 56 confirmed cases of TB, 4 of 5 suspected cases of TB, and 63 of 228 TB-negative subjects (sensitivity, 80.4%; specificity, 72.4%; accuracy, 73.9%; positive predictive value, 41.7%; negative predictive value, 93.8%). A total of 37 (78.7%) of 47 mycobacterial isolates were M. tuberculosis complex, with 75.7% from rat-positive sputa. Ten isolates were nontuberculous mycobacteria, one was M. intracellulare, one was M. avium subsp. hominissuis, and eight were unidentified. Rat-positive sputa with Moraxella catarrhalis, Streptococcus pneumoniae, Staphylococcus spp., and Enterococcus spp. were associated with TB. Rhodococcus, Nocardia, Streptomyces, Staphylococcus, and Candida spp. from rat-positive sputa did not produce M. tuberculosis-specific volatiles (methyl nicotinate, methyl para-anisate, and ortho-phenylanisole). Prevalence of Mycobacterium-related Nocardia and Rhodococcus in smear-negative sputa did not equal that of smear-negative mycobacteria (44.7%), of which 28.6% were rat positive. These findings and the absence of M. tuberculosis-specific volatiles in nonmycobacterial species indicate that rats can be trained to specifically detect M. tuberculosis. Novel methods for rapid diagnosis of tuberculosis (TB) are urgently needed to complement smear microscopy, which has low sensitivity (20), and culture, which is slower and requires specialized laboratory conditions not available in resourceconstrained settings. Trained African giant pouched rats (Cricetomys gambianus) possess profound potential for rapid detection of TB with higher sensitivity and specificity (38). An increase in TB case detection rate of 43 to 44% is achieved when Cricetomys rats are used as a second-line screening tool, after smear microscopy (18,25). Rats have a highly developed sense of smell among mammalian species (22), which renders them trainable to specifically sense TB odor in sputum samples with a broad range of acid-fast bacilli (AFB) counts (see Materials and Methods). These rats also detect smear-negative sputa, which may contain few acid-fast bacilli missed by microscopy. Moreover, smear-negative results may persi...

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“…The branched ketones 3-methylpentan-2-one (40) and (E)-3-methylpent-3-en-2-one (41) were released by many strains, while (E)-4-methylhex-4-en-3-one (42) was only found in Streptomyces afghaniensis DSM 40228. Hexan-2,4-dione (45) was the only dione and was emitted by Amycolatopsis alba DSM 44262. The occasionally detected volatiles 6-methylhept-5-en-2-one (43) and geranylacetone (44) may arise by oxidative degradation of terpenoids.…”

Section: Scheme 2 Hydrocarbonsmentioning

confidence: 99%

“…20,45 The representatives 2-furanmethanol (116), 2-(methoxymethyl)furan (117), (5-methylfuran-2-yl)methanol (118), methyl furan-2-carboxylate (119) and 2-acetylfuran (122) were widespread, while methyl 5-methylfuran-2-carboxylate (120) and 2-acetyl-5-methylfuran (123) occurred less often, and dimethyl furan-2,4-dicarboxylate (121) only in S. californicus. 20,45 The representatives 2-furanmethanol (116), 2-(methoxymethyl)furan (117), (5-methylfuran-2-yl)methanol (118), methyl furan-2-carboxylate (119) and 2-acetylfuran (122) were widespread, while methyl 5-methylfuran-2-carboxylate (120) and 2-acetyl-5-methylfuran (123) occurred less often, and dimethyl furan-2,4-dicarboxylate (121) only in S. californicus.…”

Section: Furansmentioning

confidence: 99%

Volatiles from nineteen recently genome sequenced actinomycetes

et al. 2015

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The volatiles released by agar plate cultures of nineteen actinomycetes whose genomes were recently sequenced were collected by use of a closed-loop stripping apparatus (CLSA) and analysed by GC/MS. In total, 178 compounds from various classes were identified. The most interesting findings were the detection of the insect pheromone frontalin in Streptomyces varsoviensis, and the emission of the unusual plant metabolite 1-nitro-2-phenylethane. Its biosynthesis from phenylalanine was investigated in isotopic labelling experiments. Furthermore, the identified terpenes were correlated to the information about terpene cyclase homologs encoded in the investigated strains. The analytical data were in line with functionally characterised bacterial terpene cyclases and particularly corroborated the recently suggested function of a terpene cyclase from Streptomyces violaceusniger by the identification of a functional homolog in Streptomyces rapamycinicus.

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Volatile Organic Metabolites from Selected Streptomyces Strains

Cited by 44 publications

References 15 publications

Streptomyces exploration is triggered by fungal interactions and volatile signals

Streptomyces exploration is triggered by fungal interactions and volatile signals

Diagnosis of Tuberculosis by Trained African Giant Pouched Rats and Confounding Impact of Pathogens and Microflora of the Respiratory Tract

Volatiles from nineteen recently genome sequenced actinomycetes

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