Volatile organic compounds of Bacillus atrophaeus HAB-5 inhibit the growth of Colletotrichum gloeosporioides

Rajaofera, Mamy Jayne Nelly; Wang, Yi; Dahar, Ghulam Yaseen; Jin, Pengfei; Fan, Lixia; Xu, Liangxiang; Liu, Wenbo; Miao, Weiguo

doi:10.1016/j.pestbp.2019.02.019

Cited by 44 publications

(29 citation statements)

References 32 publications

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“…The bacteria which generally showed the most effective antifungal activity, B. velezensis XT1 and B. atrophaeus L193, was found to be producers of metabolites with antifungal activity, such as lipopeptides in previous studies ( Rodriguez et al, 2018 ; Toral et al, 2018 ). Although little is known about VOCs produced by B. atrophaeus , the volatilome of B. atrophaeus HAB-5 has been identified which has a moderate antifungal effect on the common disease of anthracnose caused by the major fungus C. gloeosporioides ( Rajaofera et al, 2019 ). No information on the antifungal activity of volatile compounds produced by B. atrophaeus against the major phytopathogens analysed existed prior to our study.…”

Section: Discussionmentioning

confidence: 99%

Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens

Toral¹,

Rodríguez

Martínez‐Checa

et al. 2021

Front. Microbiol.

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Phytopathogenic fungal growth in postharvest fruits and vegetables is responsible for 20–25% of production losses. Volatile organic compounds (VOCs) have been gaining importance in the food industry as a safe and ecofriendly alternative to pesticides for combating these phytopathogenic fungi. In this study, we analysed the ability of some VOCs produced by strains of the genera Bacillus, Peribacillus, Pseudomonas, Psychrobacillus and Staphylococcus to inhibit the growth of Alternaria alternata, Botrytis cinerea, Fusarium oxysporum, Fusarium solani, Monilinia fructicola, Monilinia laxa and Sclerotinia sclerotiorum, in vitro and in vivo. We analysed bacterial VOCs by using GC/MS and 87 volatile compounds were identified, in particular acetoin, acetic acid, 2,3-butanediol, isopentanol, dimethyl disulphide and isopentyl isobutanoate. In vitro growth inhibition assays and in vivo experiments using cherry fruits showed that the best producers of VOCs, Bacillus atrophaeus L193, Bacillus velezensis XT1 and Psychrobacillus vulpis Z8, exhibited the highest antifungal activity against B. cinerea, M. fructicola and M. laxa, which highlights the potential of these strains to control postharvest diseases. Transmission electron microscopy micrographs of bacterial VOC-treated fungi clearly showed antifungal activity which led to an intense degeneration of cellular components of mycelium and cell death.

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Section: Discussionmentioning

confidence: 99%

Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens

Toral¹,

Rodríguez

Martínez‐Checa

et al. 2021

Front. Microbiol.

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“…Both these compounds are well-known microbial volatiles holding strong antagonistic activities against toxigenic as well as phytopathogenic fungi (Figure ). − …”

Section: Resultsmentioning

confidence: 99%

Biocontrol Activity of Bacillus megaterium BM344-1 against Toxigenic Fungi

et al. 2021

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Mycotoxins are secondary metabolites of some fungal species and represent important contaminants of food and feed. This study aimed to explore the biological control activity of Bacillus megaterium BM344-1 volatile organic compounds (VOCs) on the growth and mycotoxin production of single representatives of the toxigenic species Aspergillus flavus , Aspergillus carbonarius , Penicillium verrucosum , and Fusarium verticillioides . In vitro co-incubation experiments indicated the P. verrucosum isolate as the most sensitive one, with a growth inhibition ratio of 66.7%, followed by A. flavus (29.4%) and F. verticillioides (18.2%). Exposure of A. flavus , P. verrucosum, and F. verticillioides to BM344-1 VOCs resulted in complete inhibition of aflatoxins (AFB 1 , AFG 1 , and AFG 2 ), ochratoxin A, and fumonisin B 1 (FB 1 ) synthesis on artificial media, respectively. In vivo experiments on maize kernels showed 51% inhibition of fungal growth on ears simultaneously infected with A. flavus spores and exposed to BM344-1 volatiles. Likewise, AF synthesis by A. flavus was significantly ( p < 0.05) inhibited (25.34 ± 6.72 μg/kg) by bacterial volatiles as compared to that in control maize ears (91.81 ± 29.10 μg/kg). Gas chromatography–tandem mass spectrometry-based analysis of headspace volatiles revealed hexadecanoic acid methyl ester (palmitic acid) and tetracosane as bioactive compounds in the BM344-1 volatilome. Bacterial volatiles have promising potential to control the growth and mycotoxin synthesis of toxigenic fungi and may present valuable aid in the efforts to warrant food and feed safety.

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“…Oxalic acid, identified from the volatile organic compounds of Metarhizium anisopliae Ma70, has been reported as the most promising antifungal agent against B. cinerea, which causes apple gray mold [61]. Hexadecanoic acid and octadecane have been demonstrated to show antifungal activity against Colletotrichum gloeosporioides [62]. Deepthi et al [63] confirmed that 10-octadecenoic acid and heptadecanoic acid produced by Lactobacillus plantarum MYS6 are major antifungal compounds against a fumonisin-producing fungus, Fusarium proliferatum MYS9.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity and Biocontrol Mechanism of Fusicolla violacea J-1 against Soft Rot in Kiwifruit Caused by Alternaria alternata

Long

et al. 2021

JoF

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Alternaria alternata is the main pathogenic species of various crops, including kiwifruit (Actinidia cinensis). In this study, an antagonistic fungus, J-1, with high antifungal activity against A. alternata was isolated from A. cinensis “Hongyang.” The strain J-1 was identified as Fusicolla violacea via morphological identification and DNA sequencing. This study aimed to evaluate the antifungal activity and potential mechanism of the strain J-1 against A. alternata. The strain J-1 exhibited antifungal activity against A. alternata, with an inhibition rate of 66.1% in vitro. Aseptic filtrate (AF) produced by the strain J-1 could suppress the mycelial growth and conidia germination of A. alternata at the inhibition rates of 66.8% and 80%, respectively, as well as suppress the spread of Alternaria rot in fresh kiwifruit. We observed that many clusters of spherical protrusions appeared at the mycelial tips of A. alternata after treatment with 200 mL L−1 AF of J-1. Scanning electron microscopy analysis results showed that the mycelial structures were bent and/or malformed and the surfaces were rough and protuberant. Variations in temperature, pH, and storage time had little effect on the antifungal activity of the AF. Moreover, the AF could damage the integrity of cell membranes and cause intracellular content leakage. Meanwhile, the chitinase and β-1,3-glucanase enzyme activities increased significantly, indicating that the function of A. alternata cell wall was seriously injured. Eleven antimicrobial metabolites were identified by gas chromatography–mass spectrometry (GC–MS). The strain J-I and its AF exhibited well broad-spectrum antifungal activity against Diaporthe eres, Epicoccum sorghinum, Fusarium graminearum, Phomopsis sp., and Botryosphaeria dothidea, with inhibition rates ranging from 34.4% to 75.1% and 42.7% to 75.2%, respectively. Fusicolla violacea J-1 is a potential biocontrol agent against A. alternata and other fungal phytopathogens.

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Volatile organic compounds of Bacillus atrophaeus HAB-5 inhibit the growth of Colletotrichum gloeosporioides

Cited by 44 publications

References 32 publications

Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens

Identification of Volatile Organic Compounds in Extremophilic Bacteria and Their Effective Use in Biocontrol of Postharvest Fungal Phytopathogens

Biocontrol Activity of Bacillus megaterium BM344-1 against Toxigenic Fungi

Antifungal Activity and Biocontrol Mechanism of Fusicolla violacea J-1 against Soft Rot in Kiwifruit Caused by Alternaria alternata

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