Volatile Constituents from the Leaves of Callicarpa japonica Thunb. and Their Antibacterial Activities

Abstract

Volatile substances of Callicarpa japonica Thunb. were examined for their antibacterial activities against six foodborne microorganisms using the optical densitometer Bioscreen C. Extracts of C. japonica were obtained by simultaneous steam distillation and solvent extraction (SDE), and those extracted for 1.5 and 2.0 h at pH 6.0 strongly inhibited the growth of Bacillus cereus and Salmonella typhimurium; the content of the volatile substances of leaves at these pH levels were 543.1 and 706.7 mg/kg, respectively. All foodborne microorganisms tested were strongly inhibited by the addition of >8% (v/v) of the SDE extracts to broth medium. The major volatile components of the SDE extracts obtained at 1.5 h and pH 6.0 were gamma-caryophyllene, 1-octen-3-ol, 2-hexenal, germacrene B, and aromadendrene II, with corresponding peak areas of 44.14, 15.6, 9.86, 5.24, and 4.01%, respectively, and major antibacterial components were 1-octen-3-ol and 2-hexenal. Among the 32 materials identified as volatile flavor components, 2-hexenal, 2,4-hexadienal, 1-octen-3-ol, 2,4-heptadienal, and epiglobulol strongly inhibited microorganism growth. In particular, 2-hexenal (107.52 mg/L) and 1-octen-3-ol (678.64 mg/L) inhibited the growth of most microorganisms tested by >90%.

Screening of Antibacterial Activities of Twenty-One Oxygenated Monoterpenes

“…The data presented in Table I show that α-terpineol was active against S. enteritidis ATCC 13076. It has been also reported that linalool, menthol and α-terpineol were less potent against E. coli and Salmonella typhimurium (Sivropoulou et al, 1995;Kim and Shin, 2004). The similar results for these compounds were found in the present study.…”

“…Recently, a large number of investigations has been performed on the antibacterial activities of plant essential oils, but not of the major components (Kim et al, 1995;Sivropoulou et al, 1995;Pattnaik et al, 1997;Lis-Balchin et al, 1998;Dorman and Deans, 2000;Friedman et al, 2002Friedman et al, , 2004Iscan et al, 2002;Oumzil et al, 2002;Gulluce et al, 2003;Karaman et al, 2003;Sahin et al, 2003;Kim and Shin, 2004). On the other hand, this is the first time that the antibacterial activities of pure oxygenated monoterpenes have been screened toward bacterial species of plant, food and clinic origins.…”

“…Many essential oils isolated from various plant species belonging to different genera contain relatively high amounts of oxygenated monoterpenes. Antibacterial activities of numerous essential oils and their major components have extensively been studied (Kim et al, 1995;Sivropoulou et al, 1995;LisBalchin et al, 1998;Hammer and Carson, 1999;Iscan et al, 2002;Oumzil et al, 2002;Gulluce et al, 2003;Friedman et al, 2004;Kim and Shin, 2004;Sokmen et al, 2004). However, in these reports, a limited number of their pure major components was tested for antibacterial activities against a limited number of microorganisms.…”

Morphoanatomic aspects and phytochemical screening of Plinia edulis (Vell.) Sobral (Myrtaceae)

“…The antibacterial and antifungal activity of several plants have been attributed to terpenoids (Wyatt et al, 2005;Filipowics et al, 2003) and phenolics (Heinonen, 2007) such as tannin, responsible for the astringent taste of P. edulis leaves. Despite of the fact that these classes of compounds had shown antibiotic activity against some types of strains (Heinonen, 2007;Kim, Shin, 2004;Kubo, Muroi, Kubo, 1993) …”

Detection of potential volatile inhibitory compounds produced by endobacteria with biocontrol properties towards Fusarium oxysporum f. sp. cubense race 4

“…In contrast, the volatile compounds 2-butanone 3-methyl, dimethyl sulfide, 1-dodecene, ethyl acetate and 1-nonanol, were classified as non-antifungal specific towards FocR4 only. This is because these compounds have been documented to have either antibacterial or antifungal properties towards several other tested pathogens other than FocR4, therefore their antimicrobial potential is not entirely ruled out (Hamilton-Kemp et al 1996;Kamigiri et al 2004;Kim and Shin 2004;Brown and Morra 2005;Druvefors et al 2005;Yayli et al 2006).…”