Volatile Components of Roots, Stems, Leaves, and Flowers of <i>Echinacea</i> Species

Mazza, G.; Cottrell, Tony

doi:10.1021/jf981117y

Cited by 53 publications

(41 citation statements)

References 7 publications

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“…PcISPS promoter is active in roots, however, they do not emit isoprene Many studies show the presence and emission of VOC in roots and their emission as a plant defence reaction against herbivores (Mazza and Cottrell 1999;Köllner et al 2004;Rasmann and Turlings 2007). Less information exists about the genes involved in these root emissions.…”

Section: Developmental Control Of Isoprene Emission In Grey Poplar Stmentioning

confidence: 99%

Regulation of isoprene synthase promoter by environmental and internal factors

Cinege¹,

Louis

Hänsch

et al. 2008

Plant Mol Biol

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Isoprene synthase (ISPS) catalyzes the formation of isoprene, an important volatile terpenoid with strong effects on global atmospheric chemistry and protective physiological functions in plant leaves. Many terpene synthase genes including isoprene synthase, a member of the TPS-b cluster of this numerous gene family, were already functionally analysed but much less is known about regulation of their promoters. To study regulation of the PcISPS gene in detail we developed transgenic Grey poplar (Populus x canescens) and Arabidopsis thaliana plants in which the PcISPS promoter is fused to enhanced green fluorescent protein (E-GFP) and beta-glucuronidase (GUS) reporter genes. We analysed these reporters during plant development, for organ specificity and in plants subjected to different light and temperature regimes. We observed low promoter activity in non-isoprene emitting tissue like roots where ISPS gene is transcribed but no active enzyme is detectable. In leaves we demonstrate that light and temperature directly modulate ISPS promoter activity. Moreover, with confocal laser scanning microscopy we show a cell specific gradient of ISPS promoter activity within the leaf parenchyma depending on light direction. Our results indicate that ISPS promoter activity, which correlates with basal isoprene emission capacity, is not uniformly distributed within leaf tissue and that it can adapt rapidly towards internal as well as external environmental stimuli.

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Section: Developmental Control Of Isoprene Emission In Grey Poplar Stmentioning

confidence: 99%

Regulation of isoprene synthase promoter by environmental and internal factors

Cinege¹,

Louis

Hänsch

et al. 2008

Plant Mol Biol

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“…Nevertheless, significant differences, both qualitative and quantitative, in the chemical composition of the three species were reported (Mazza & Cotrell 1999;Sloley et al 2001, Perry et al 2001Speroni et al 2002) and the chemical profile, in some cases, has been correlated with a different pharmacological activity (Speroni et al 2002) enabling more differentiated and appropriate therapeutic use of these plant species.…”

Section: Introductionmentioning

confidence: 99%

Morphology and anatomy of Echinacea purpurea, E. angustifolia, E. pallida and Parthenium integrifolium

Mistríková

Vaverková

2007

Biologia

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The Echinacea species are native to the Atlantic drainage area of the United States of America and Canada. They have been introduced as cultivated medicinal plants in Europe. Echinacea purpurea, E. angustifolia and E. pallida are the species most often used medicinally due to their immune-stimulating properties. This review is focused on morphological and anatomical characteristics of E. purpurea, E. angustifolia, E. pallida, because various species are often misidentified and specimens are often confused in the medicinal plant market.

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“…Although high temperatures will yield extracts containing more components, thermal decomposition can occur at high temperatures [1]. This method has been used to extract volatile compounds from different plant materials [4,5].…”

Section: Introductionmentioning

confidence: 99%

Headspace solid-phase microextraction analysis of volatile components of spices

2002

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SummaryHeadspace solid-phase microextraction (HSSPME) coupled with gas chromatography-mass spectrometry (GC-MS) has been used to isolate volatile compounds from three different spices (basil, oregano, and bay leaves). SPME conditions were optimized before the experimental extractions, to achieve optimum recovery of the volatile compounds. Method reproducibility and linearity were evaluated. Relative standard deviations (RSD) bel',,veen 1.4 and 9.3% were obtained. Typical components of the essential oils of spices and other plant materials, e.g. terpenes, hydrocarbons, and oxygenated terpenes were identified. These results indicate that headspace SPME coupled with GC-MS is an excellent method for analysis of the odors of spices.

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Volatile Components of Roots, Stems, Leaves, and Flowers of Echinacea Species

Cited by 53 publications

References 7 publications

Regulation of isoprene synthase promoter by environmental and internal factors

Regulation of isoprene synthase promoter by environmental and internal factors

Morphology and anatomy of Echinacea purpurea, E. angustifolia, E. pallida and Parthenium integrifolium

Headspace solid-phase microextraction analysis of volatile components of spices

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