Volatile components and antimicrobial activity of the n-hexane extracts of Neomuretia pisidica (Kit Tan) Kljuykov, Degtjareva & Zakharova

Abstract

The fruits, aerial parts and roots of Neomuretia pisidica (Kit Tan) Kljuykov, Degtjareva & Zakharova were extracted with n-hexane. Total of 18 compounds were characterised by GC analyses of the n-hexane extracts. Main volatile components of the n-hexane extract of aerial parts were characterized as 1,8-cineole (23.4%), camphor (21.4%), 2-ethyl hexanol (14.6%), α-pinene (7.2%), and verbenone (6.4%). Methyl linoleate (19.3%), 1,8-cineole (16.5%), camphor (13.2 %), α-pinene (6.1 %) and 2-ethyl hexanol (4.9%) were found in the n-hexane extract of roots. Whereas, 1,8-cineole (23.3 %), camphor (20.3%), 2-ethyl hexanol (14.2 %), α-pinene (9.9%), and limonene (4.1%) were the major components of the n-hexane extract of fruits. Antimicrobial activity were identified using a microdilution assay against selected human pathogenic strains. The most potent inhibitor activities with 156 µg/mL concentrations were detected against S. aureus and E. faecalis.

Antibacterial and phytochemical constituent of Etlingera rubroloba A.D. Poulsen extract, an endemic ginger from Wallacea Region, Indonesia

“…Fifty grams of powdered plant material of E. rubroloba were prepared in 3 replicates each, then each extract was put into an Erlenmeyer flask and soaked in 500 mL n-hexane solvent, then placed at room temperature for 7 days. Hexane is a polar solvent that can bind polar compounds including volatile compounds and terpenes which show strong antibacterial activity (Karadağ et al 2021). The results of maceration with n-hexane solvent were filtered using Whatman filter paper, then evaporated by a Vacuum rotary evaporator IKA rotary evaporator RV 3 V at 45 o C. The extracts obtained were stored in a refrigerator at 4°C before being used for further analysis.…”