Volatile C8 compounds and pseudomonads influence primordium formation of<i>Agaricus bisporus</i>

Noble, R.; Dobrovin‐Pennington, Andreja; Hobbs, P. J.; Pederby, Jemma; Rodger, Alison

doi:10.3852/07-194

Cited by 86 publications

(80 citation statements)

References 17 publications

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“…It is interesting to note that P. putida was abundant on fresh mushrooms but not on stored ones, simulating a ready-to-sell product. This bacterium is well known for its role in fruit body initiation (Hayes et al 1969;Noble et al 2009) and our results indicated that it was not part of the postharvest spoilage syndrome associated with pseudomonads. The presence of pseudomonads and E. americana in mushrooms is in agreement with reports from different studies.…”

Section: Discussionmentioning

confidence: 58%

Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product

et al. 2015

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The aim of the study was to investigate microbial succession in the mushroom supply chain from compost, casing to fruit body formation and mushroom growth to the point of harvested, packing and point of sale. The microbial population dynamics of compost, casing and mushrooms were determined using a plate count technique, denaturing gradient gel electrophoresis (DGGE) and sequencing of 16S and 18S rDNA. Plating revealed greater abundance of bacteria, fungi and yeasts in mushroom compost compared to casing and fresh mushroom samples. The viable count method also showed that bacteria and yeasts increased significantly after harvest and Page 2 of 38 during cold storage. Sequencing revealed a more diverse culturable bacterial population in casing and on the mushrooms than in the compost. Phylogenetic analysis revealed a general trend of grouping of species from the same sources. In contrast, a higher microbial diversity was recorded in compost when using the DGGE method, which reflects cultural and non-culturable microorganisms. For compost and casing bacteria studied using DGGE, several species formed separate lineages, demonstrating highly diverse communities in these samples. Fungi were shown to be less abundant and less diverse compared to bacteria and yeasts. The study provides baseline knowledge of microbial populations and -succession trends in mushroom production systems using viable and non-viable methods. The information provided in this study may be useful for microbial ecology studies and to identify and develop biocontrol systems for pathogen control during production or to enhance pinning stimulation by knowing when to apply Pseudomonas spp. to ensure increased yield. Finally an insight is provided into microbial survival during cold storage and marketing of mushrooms. Potential antagonistic populations known to prevent spoilage, quality deterioration and extend shelf life are listed in this paper.

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Section: Discussionmentioning

confidence: 58%

Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product

et al. 2015

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“…Addition of bacteria isolated from casing and mycelium could increase the initiation of basidiocarps (Reddy and Patrick 1990) and primordial inhibitors either metabolized by the casing microbiota (P. putida) or absorbed by certain casing materials (Flegg and Wood 1985). It has also shown that 2-ethyl-1-hexanol and 1-octen-3-ol volatile compounds increased total bacterial and pseudomonad populations in the casing (Noble et al 2009). These bacteria were able to metabolize the 1-octen-3-ol inhibitory compound, as previously also shown by Hayes et al (1969) andEger (1972).…”

Section: Discussionmentioning

confidence: 96%

Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production

Zarenejad

Yakhchali

Rasooli

2011

World J Microbiol Biotechnol

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Mushrooms such as Agaricus bisporus, are cultivated for food worldwide. Fruit body initiation in Agaricus bisporus is a phase change from the vegetative to the reproductive stage which depends on the presence of a casing layer with particular physical, chemical and microbiological properties. The phase change is achieved practically by environmental manipulation and the presence of naturally occurring bacteria such as Pseuodomonas putida. In this study, 274 individual bacterial isolates were collected by screening the casing layer of 14 edible mushroom farms. The isolates were analysed with respect to biochemical properties, organic and inorganic phosphate solubilization, production of siderophore and growth in the presence of volatile compound of 1-octen-3-ol. It was found that approximately 97% of the strains were able to grow in the presence of 1-octen-3-ol and 36% were able to solubilize phosphorus. Among the isolates, 23 strains were selected as potent mushroom growth promoting bacteria (MGPB) for inoculation of the casing layer. Field experiments using these strains showed various promoting effects on production of mushroom. Finally, 2 strains (strains Bt4 and Ps7) showing the highest increase in A. bisporus production, were characterized as Pseuodomonas putida by molecular methods and identified as the best suited growth promoting inoculants for application in production farms for increasing the mushroom yield.

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“…For example, under conditions of high spore density, 1-octen-3-ol functions as a self-inhibitor in Penicillium paneum and Agaricus bisporus [11,12,52]. Selfinhibitors inhibit spore germination reversibly, possibly through effects on the plasma membrane [12].…”

Section: Introductionmentioning

confidence: 97%

“…At certain concentrations, the eight-carbon oxylipins 1-octen-3-ol, 3-octanone, and 3-octanol stimulate aerial conidiation in Aspergillus nidulans [26]. In Agaricus bisporus, 1-octen-3-ol inhibits primordium formation [52]. 1-Octen-3-ol has also been used to minimize dry bubble disease caused by Lecanicillium fungicola in the cultivation of the white button mushrooms [3].…”

Section: Introductionmentioning

confidence: 99%

Effects of Three Volatile Oxylipins on Colony Development in Two Species of Fungi and on Drosophila Larval Metamorphosis

et al. 2015

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The aim of this study is to investigate the effects of three volatile oxylipins on colony development in two fungi and on Drosophila larval metamorphosis. Using an airborne exposure technique, three common and volatile oxylipins (1-octen-3-ol, (E)-2-hexenal, and 1-hexanol) were compared for their effects on spore germination and colony growth in Aspergillus niger and Penicillium chrysogenum, as well as for their effects on the morphogenesis of larvae of Drosophila melanogaster. Conidia of both A. niger and P. chrysogenum plated in the presence of low concentrations (50 ppm) of these three volatile organic compounds (VOCs) formed fewer colony-forming units (CFUs) and exhibited reduced radial growth of colonies as compared to controls. When A. niger and P. chrysogenum spores were germinated in the presence of the enantiomers of 1-octen-3-ol, (R)-(-)-1-octen-3-ol had the greatest impact on colony morphology (decreased sporulation and colony diameter), while (S)-(+)-1-octen-3-ol and the racemic form yielded similar morphological changes but to a lesser extent. In addition, Drosophila larvae exposed to vapors of these oxylipins exhibited serious delays in metamorphosis and toxic effects on pupae and adult stages. Low concentration of these three VOCs can significantly inhibit the formation of CFUs and the growth of fungi. (R)-(-)-1-octen-3-ol imposed the greatest impact on fungal morphology compared to (S)-(+)-1-octen-3-ol and the racemic form. The three volatile oxylipins could also delay the metamorphosis of Drosophila and impose toxic effects on its pupae and adult stages.

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Volatile C8 compounds and pseudomonads influence primordium formation ofAgaricus bisporus

Cited by 86 publications

References 17 publications

Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product

Microbial succession in white button mushroom production systems from compost and casing to a marketable packed product

Evaluation of indigenous potent mushroom growth promoting bacteria (MGPB) on Agaricus bisporus production

Effects of Three Volatile Oxylipins on Colony Development in Two Species of Fungi and on Drosophila Larval Metamorphosis

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