Volatile Bioactive Compounds from Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Residing in the Leaf of Ocimum sanctum

Abstract

Endophytic fungi are known as potential novel compound producers with promising antimicrobial activities. Hence, the present study aimed to investigate the possible bioactive compounds present in the ethyl acetate extract of Lasiodiplodia pseudotheobromae IBRL OS-64. The ethyl acetate extract exhibited significant antibacterial activity against both Gram-positive and Gram-negative bacteria in disc diffusion assay. Thin-layer chromatography (TLC) was performed with chloroform, acetone and ethyl acetate (1:2:1, respectively) used as a solvent system and nine spots with diverse polarities were obtained. The TLC chromatogram with the active spot was localized with bioautography assay and the finding revealed that the dark spot with an Rf value of 0.5882 showed good antibacterial activity against all test bacteria. The fraction F5 exhibited promising antibacterial activity upon partial purification of dark spot via column chromatography and the GC-MS analysis of fraction F5 resulted in the detection of a major compound, 2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester with 90% matching factor. Thus, this compound may greatly contribute to the antibacterial activity of the fraction and has the potential to be developed as an antibiotic. The findings indirectly indicate that fungal endophytes from the medicinal plant could be a potential candidate for bioactive compounds with pharmaceutical properties.

Fungicidal and plant growth-promoting traits of Lasiodiplodia pseudotheobromae, an endophyte from Andrographis paniculata

“…By modifying bacterial cells and limiting their proliferation, ethyl acetate extract of L. pseudotheobromae IBRL OS-64, an endophytic fungus from the leaf of Ocimum sanctum was active against Methicillin-resistant Staphylococcus aureus. The growth of both Gram-positive and Gram-negative bacteria was significantly suppressed (Jalil and Ibrahim, 2022). Indole-3-acetic acid (IAA) is a kind of auxin that was associated with plant growth.…”

An Investigation of the Protective Influence of Dates Fruit Against Nephrotoxicity in Wistar Albino Rats Induced by Gentamicin

“…Diisooctyl adipate has toxicological properties in biological fluids and tissues [44], 1,2-Benzenedicarboxylic acid has shown promising results as a chemopreventive/chemotherapeutic agent against osteosarcoma [45], diisooctyl ester, which has antioxidant and anti-inflammatory properties [46], Z,E-3,13-octadecadien-1-ol, which has antioxidant properties [47].When eicosane exhibited higher antibacterial, antifungal, and antioxidant activity [48].The heptadecane, 2,6,10,15-tetramethyl-, beneficial as a promising source of antimicrobial and antioxidant therapeutic agents [49], oxirane, hexadecyl-, ntimicrobial compounds [50]and benzenepropanoic acid phytochemicals with antimicrobial and anticancer properties [51], Hence, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, antimicrobial activities ( [52],octadecyl ester, the promising inhibition efficiency of these compounds against the pathogenic bacteria [53]. The antimicrobial and antioxidant compounds [54], 13-tetradecen-1-ol acetate, and 2-pentadecanone, antioxidants [55], 6,10,14-trimethyl-2-Octadecyl-propane-1,3-diol the findings suggest that fungal endophytes from the medicinal plant could be a potential candidate for bioactive compounds with pharmaceutical properties [56] Many studies have used this plant as a preventive agent against GM nephrotoxicity and have obtained good results due to antioxidant content in plants [57,58]. Previous results support our findings, which indicate that date fruit has high contents of antioxidants and phytochemicals.…”

Antagonistic Activity of Endophytic Fungi Isolated From Aloe Vera Leaves Against Some Plant Pathogenic Fungi

“…The compound 1-Monolinoleoylglycerol trimethylsilyl ether is among the compounds present in the alcoholic extract of cyanobacteria, the extract showed antioxidant and antimicrobial activities and it induces apoptosis in human liver cancer cell lines in vitro (10). Hexanedioic acid, bis(2ethylhexyl) ester is one of the compounds illustrated by the GC-MS assay of the alcoholic extract of Lasiodiplodia pseudotheobromae, the extract showed antibacterial activities against both Grampositive and Gram-negative bacteria (23). The compound -Pyrimidine, 4,6-dimethoxy-5-nitro was among the compounds detected by GC-MS examination of the methanolic extract of Crotalaria longirostrata leaves, the extract showed activity against the nymph insect pest Bactericera cockerelli (31).…”