Vocal fold proteoglycans and their influence on biomechanics

Gray, Steven D.; Titze, Ingo R.; Chan, Roger W.; Hammond, Thomas H.

doi:10.1097/00005537-199906000-00001

Cited by 257 publications

(304 citation statements)

References 33 publications

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“…Decorin is a small chain proteoglycan, which is normally distributed in the superficial layer of the lamina propria of human vocal folds. 17 Decorin acts to regulate collagen fibrils and also inhibits formation of collagen fibers 15 , suggesting that decorin may reduce scar effects. A rabbit study reported a decrease of decorin in two-month scars of rabbit vocal folds.…”

Section: Discussionmentioning

confidence: 99%

“…Elastica-van-Gieson staining was used to identify collagen and elastin, and Alcian blue staining was used for HA after digestion with hyaluronidase. This technique was developed by Gray et al 15 to correctly identify HA in the vocal fold lamina propria. To perform hyaluronidase digestion, 50 mg of bovine testicular hyaluronidase (Sigma, St Louis, MO) was diluted in 100 mL of phosphate-buffered saline (PBS) and sections were incubated in the PBS-hyaluronidase mixture for 1 hour at 37 o C. Then the sections were stained with Alcian blue (pH 2.5).…”

Section: Histologic Examinationmentioning

confidence: 99%

“…15 It is one of the glycosaminoglycans that are widely distributed in the superficial layer of the lamina propria. It has been confirmed that HA contributes to fetal scarless wound healing, 18 which suggests that HA may have a potential role in treatment and prevention of scarring.…”

Section: Human Scarred Vocal Folds 11mentioning

confidence: 99%

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Histologic Characterization of Human Scarred Vocal Folds

et al. 2009

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Objectives: Vocal fold scarring remains a significant problem. Although several animal models have been developed to improve our understanding of the histopathology, the histologic features of scarred human vocal folds have rarely been reported. The present case studies aimed to define the histologic changes of scarred human vocal folds caused by cordectomy or cordotomy.Methods: Ten patients with the scarred vocal folds were involved in this study. Nine patients with early glottic cancer underwent endoscopic cordectomy, and one patient underwent superficial cordotomy for idiopathic scar. The post-cordectomy or cordotomy scar was biopsied or resected 3 to 13 months after the original procedure. After confirming absence of any tumor in cancer patients, the remaining specimens were used in the present study. Histologic examination investigated deposition of extracellular matrix (ECM) including collagen, elastin, hyaluronic acid (HA), fibronectin, and decorin in the lamina propria of the scarred vocal folds.Results: There was a wide range variation in the deposition of ECM in scarred vocal folds.Excessive and disorganized collagen deposition was observed in most cases that had undergone deep resection of the lamina propria, whereas deposition of collagen was mild and well organized after superficial resection. Decorin was retained in all cases after superficial cordectomy or cordotomy, but varied after deep resection. Deposition of elastin, HA, and fibronectin varied regardless of depth of injury. Conclusion:Histology of scarred vocal folds may vary with degree of injury and individual healing mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Histologic Examinationmentioning

confidence: 99%

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Histologic Characterization of Human Scarred Vocal Folds

et al. 2009

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“…In this pilot clinical trial hyaluronic acid-based (HA) scaffolds (HYAFF Ò 11) were used as a cell-carrier material. This naturally occurring polysaccharide is a major component of the ECM in connective tissues [15], plays a prominent role in cellular behaviour and exerts an important mechanical role [16]. Being a polysaccharide and not a protein it is potentially less antigenic, which is an important property when considering clinical uses.…”

Section: Introductionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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a b s t r a c tHistioconductive approaches to soft-tissue defects use scaffolds seeded with lineage-and tissue-specific progenitors to generate tissue which should reside in equilibrium with adjacent tissue. Scaffolds guide histiogenesis by ensuring cell-cell and cell-matrix interactions. Hyaluronic acid-based (HA) preadipocyte-seeded scaffolds were evaluated for their adipo-conductive potential and efficacy in humans. Preadipocytes were isolated from lipoaspirate material and seeded on HA scaffolds. The cellular biohybrid (ADIPOGRAFT Ò ) and an acellular control scaffold (HYAFF Ò 11) were implanted subcutaneously. At specific time points (2, 8 and 16 weeks) explants were analyzed histopathologically with immunohistochemistry. No adverse tissue effects occurred. Volume loss and consistent degradation of the HYAFF Ò 11 scaffolds compared to the ADIPOGRAFT Ò group indicated progressive tissue integration. No consistent histological differences between both groups were observed. By 8 weeks all void spaces within the scaffolds were filled with cells with pronounced matrix deposition in the ADIPOGRAFT Ò bio-hybrids. Here we show that HA scaffolds were stable cell carriers and had the potential to generate volumeretaining tissue. However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.

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“…Specifically, we examined (i) the actions of the dynamic strain on IL-1β-induced proinflammatory gene induction in vocal fold fibroblasts, (ii) the time, magnitude, and frequency-dependent effects of dynamic mechanical signals on vocal fold fibroblasts, and (iii) the actions of dynamic strain on the production of collagen type I (alpha1) required for tissue repair and critical for the biomechanical properties of the vocal folds in the presence or absence of IL-1β. 25,26 Understanding the actions of mechanical signals on vocal fold fibroblasts would facilitate the development of therapeutic strategies to enhance resolution of vocal fold inflammation and wound healing following injury, based on sound scientific evidence.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Biomechanical Strain Inhibits IL-1β–induced Inflammation in Vocal Fold Fibroblasts

et al. 2007

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SummaryDespite the fact that vocal folds are subjected to extensive mechanical forces, the role of mechanical strain in vocal fold wound healing has been overlooked. Recent studies on other tissues have demonstrated that low physiological levels of mechanical forces are beneficial to injured tissues, reduce inflammation, and induce synthesis of matrix-associated proteins essential for enhanced wound healing. In this study, we speculated that mechanical strain of low magnitudes also attenuates the production of inflammatory mediators and alters the extracellular matrix synthesis to augment wound healing in cultured vocal fold fibroblasts. To test this hypothesis, fibroblasts from rabbit vocal folds were isolated and exposed to various magnitudes of cyclic tensile strain (CTS) in the presence or absence of interleukin-1β (IL-1β). Results suggest that IL-1β activates proinflammatory gene transcription in vocal fold fibroblasts. Furthermore, CTS abrogates the IL-1β-induced proinflammatory gene induction in a magnitude-dependent manner. In addition, CTS blocks IL-1β-mediated inhibition of collagen type I synthesis, and thereby upregulates collagen synthesis in the presence of IL-1β. These findings are the first to reveal the potential utility of low levels of mechanical signals in vocal fold wound healing, and support the emerging on vivo data suggesting beneficial effects of vocal exercise on acute phonotrauma.

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Vocal fold proteoglycans and their influence on biomechanics

Abstract: Proteoglycans, particularly hyaluronic acid, play important roles in determining biomechanical properties of tissue oscillation. Future research will likely make these proteins of important therapeutic interest.

Cited by 257 publications

References 33 publications

Histologic Characterization of Human Scarred Vocal Folds

Histologic Characterization of Human Scarred Vocal Folds

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

Dynamic Biomechanical Strain Inhibits IL-1β–induced Inflammation in Vocal Fold Fibroblasts

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