VNTR analysis of selected outbreaks of Burkholderia pseudomallei in Australia

Pearson, Talima; U’Ren, Jana M.; Schupp, James M.; Allan, Gerard J.; Foster, Peter G.; Mayo, Mark; Gal, Daniel; Choy, Jodie Low; Daugherty, Rebecca L.; Kachur, Sergey; Friedman, Christine L Clark; Leadem, Benjamin R.; Georgia, Shalamar; Hornstra, Heidie; Vogler, Amy J.; Wagner, David M.; Keim, Paul; Currie, Bart J.

doi:10.1016/j.meegid.2006.12.002

Cited by 32 publications

(44 citation statements)

References 22 publications

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“…cenocepacia is a member of a highly adaptable genus of bacteria that live under diverse circumstances in nature (36) and that can rapidly evolve under in vitro stress conditions or during infections (46,125,128). This adaptability may be key to the pathogenesis of B. cenocepacia, especially to the ability of these bacteria to establish opportunistic chronic infections (167).…”

Section: Discussionmentioning

confidence: 99%

“…These elements are thought to contribute to the plasticity of Burkholderia genomes and their ability to acquire a wide range of metabolic pathways (93). Burkholderia genomes can also mutate rapidly when the organisms are subject to in vitro stress conditions or during infections (46,125,128). The genome sequence of B. cenocepacia strain J2315 was published in 2009 (65), although the Wellcome Trust Sanger Institute made initial sequencing and annotation of the genome available to researchers since 2000.…”

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confidence: 99%

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A Decade of Burkholderia cenocepacia Virulence Determinant Research

2010

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The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. These infections are difficult to treat due to the inherent resistance of the bacteria to antibiotics. Bacteria can spread between CF patients through social contact and sometimes cause cepacia syndrome, a fatal pneumonia accompanied by septicemia. Burkholderia cenocepacia has been the focus of attention because initially it was the most common Bcc species isolated from patients with CF in North America and Europe. Today, B. cenocepacia, along with Burkholderia multivorans, is the most prevalent Bcc species in patients with CF. Given the progress that has been made in our understanding of B. cenocepacia over the past decade, we thought that it was an appropriate time to review our knowledge of the pathogenesis of B. cenocepacia, paying particular attention to the characterization of virulence determinants and the new tools that have been developed to study them. A common theme emerging from these studies is that B. cenocepacia establishes chronic infections in immunocompromised patients, which depend more on determinants mediating host niche adaptation than those involved directly in host cells and tissue damage.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

A Decade of Burkholderia cenocepacia Virulence Determinant Research

2010

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“…Using a 32 VNTR system, U'Ren et al showed extensive diversity within a global B. pseudomallei isolate set (26). When 30 of these VNTR loci were used to analyze 9 epidemiologically related B. pseudomallei isolate sets, fi ne-scale diversity was found even among closely related strains, including sequential isolates from persons (13). We sought to develop a rapid and robust minimum loci B. pseudomallei MLVA that differentiated unrelated strains and maintained the ability to link isolates from a point-source outbreak.…”

Section: Discussionmentioning

confidence: 99%

“…Thirty of these VNTR markers were subsequently used for fi ne-scale analysis of 121 isolates of B. pseudomallei (13). Various combinations of markers were tested by MLVA; we chose 4 markers that were highly discriminatory, enabling singlerun, 4-color analysis in a DNA sequencer.…”

Section: Mlva Pfge and Mlstmentioning

confidence: 99%

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Currie¹,

Haslem²,

Pearson³

et al. 2009

Emerg. Infect. Dis.

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Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsedfi eld gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplifi ed 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal outbreak of melioidosis has occurred within 8 hours of receipt of bacterial strains.

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“…Due to the limitations of MLST in determining the associations between STs, further refinement of the estimates of geographic origin could not be performed using this technique alone. MLVA is known to be highly accurate for defining isolate associations, and has previously been used to differentiate closely associated bacterial strains within outbreaks (19). Therefore, in the present study MLST and MLVA-4 were employed to achieve optimal molecular epidemiologic confidence for this unusual case of melioidosis.…”

Section: Discussionmentioning

confidence: 99%

Molecular tracking investigation of melioidosis cases reveals regional endemicity in Hainan, China

et al. 2016

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Abstract. Sporadic cases of melioidosis have been reported in Hainan, China for decades; however, to the best of our knowledge, there are no accurate source-identification investigations confirming that melioidosis is endemic. Four indigenous melioidosis cases were identified, which prompted the performance of contact microbiologic and molecular techniques to evaluate endemicity. Environmental samples were collected from various locations surrounding each patient's residence. The samples were screened for Burkholderia pseudomallei (B. pseudomallei) using Ashdown culture medium, and confirmed by polymerase chain reaction and 16S ribosomal DNA sequencing. Clinical and environmental isolates of B. pseudomallei were evaluated by multilocus sequence typing (MLST) and 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for evidence of homology between them. Analysis by MLST indicated that one environmental sample and one clinical colony were sequence type-46, as well as type (8,3,11,9) by MLVA-4. The evidence indicates a likely geographical and epidemiological association. Taken together, B. pseudomallei from the environmental samples in addition to the high molecular homology between the clinical and environmental isolates indicates, at least, regional endemicity of melioidosis in Hainan, China.

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VNTR analysis of selected outbreaks of Burkholderia pseudomallei in Australia

Cited by 32 publications

References 22 publications

A Decade of Burkholderia cenocepacia Virulence Determinant Research

A Decade of Burkholderia cenocepacia Virulence Determinant Research

Identification of Melioidosis Outbreak by Multilocus Variable Number Tandem Repeat Analysis

Molecular tracking investigation of melioidosis cases reveals regional endemicity in Hainan, China

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