Vmp1 and stamp genes variability of ‘Candidatus phytoplasma solani’ in Bosnian and Herzegovinian grapevine

Delić, Duška; Balech, Bachir; Radulović, Mariana; Lolić, B.; Karačić, Ana; Vukosavljevic, V.; Đurić, Gordana; Cvetković, Tatjana Jovanović

doi:10.1007/s10658-015-0828-z

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…We have confirmed the detection of the vmp1 molecular types V1, V4, V9, and V11 via PCR-RFLP, sequence analysis and virtual digestion. These types are known to be present in Sicily and also in northwestern Italy, central eastern Italy, and Sardinia [7,[43][44][45], and across different European countries [3,14,21,[46][47][48]. In the present study, V9 was the most frequent vmp1 molecular type, in contrast to Oliveri et al [44], who reported the most frequent as V1.…”

Section: Discussioncontrasting

confidence: 71%

Epidemiological Investigations and Molecular Characterization of ‘Candidatus Phytoplasma solani’ in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily (Southern Italy)

et al. 2020

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Bois noir is caused by ‘Candidatus Phytoplasma solani’, and it is one of the most important and widespread diseases in the Euro-Mediterranean region. There are complex interactions between phytoplasma and grapevines, weeds, and vectors. These ecological relationships can be tracked according to molecular epidemiology. The aims of the 2-year study (2014–2015) were to describe incidence and spatial distribution of Bois noir in a vineyard with three grapevine varieties in Sicily, and to identify the molecular types of the tuf and vmp1 genes in these naturally infected grapevines, according to the potential reservoir plants and vectors. Disease incidence in 2015 was significantly higher in ‘Chardonnay’ (up to 35%) than for ‘Nero d’Avola’ and ‘Pinot noir’ (<5%). All grapevine, weed, and insect samples were infected by ‘Ca. P. solani’ tuf-type b. Most of the collected insects were strictly related to Vitis spp. and belonged to Neoaliturus fenestratus, Empoasca spp., and Zygina rhamni. The characterization of the vmp1 gene revealed six different vmp types in grapevines (V1, V4, V9, V11, V12, V24), three in weeds (V4, V9, V11), and four in insects (V4, V9, V11, V24). Notably, V4, V9, appear both in hosts and vectors, with V9 predominant. Virtual restriction fragment length polymorphism (RFLP) analysis based on the nucleotide sequences supported the data of the conventional RFLP. Connections between the molecular data recorded in the vineyard ecosystems and the application of innovative tools based on the geostatistical analysis will contribute to further clarification of the specific ecological and epidemiological aspects of ‘Ca. P. solani’ in Sicily.

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Section: Discussioncontrasting

confidence: 71%

Epidemiological Investigations and Molecular Characterization of ‘Candidatus Phytoplasma solani’ in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily (Southern Italy)

et al. 2020

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“…P. solani’ studies; it was found to be useful to study and monitor the epidemiology of ‘ Ca. P. solani’ [ 33 , 36 , 46 , 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

Multilocus Genotyping of ‘Candidatus Phytoplasma Solani’ Associated with Grapevine Bois Noir in Iran

Jamshidi

Murolo

Ravari

et al. 2022

Biology

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Grapevine Bois noir (BN) is associated with ‘Candidatus Phytoplasma solani’. It has been recorded in vineyards throughout Europe as well as in different countries in Asia, where it now constitutes a threat to Iranian viticulture. BN is strictly dependent on ‘Ca. P. solani’ strains, wild host plants, and insect vectors. The molecular typing of ‘Ca. P. solani’, based on the nonribosomal gene tuf and the two hypervariable markers vmp1 and stamp, is valuable for the reconstruction and clarification of the pathways of BN spread. In this study, an RFLP analysis was performed on the vmp1 gene, and a single-nucleotide polymorphism analysis confirmed new vmp types in ‘Ca. P. solani’. A stamp gene phylogenetic analysis allowed us to distinguish between the new genotype infections in the grapevines and the ‘weeds’ Convolvulus arvensis and Erigeron bonariensis in Iranian vineyards, highlighting the close genetic relatedness of the strains of ‘Ca. P. solani’ found in Iran and Azerbaijan. The most common genotype in the grapevines was tuf b/V24/stamp III, which was associated with C. arvensis. This information contributes toward the identification of further routes of introduction of ‘Ca. P. solani’ in Iran to sustain the control measures for the management of BN.

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“…Considering the vmp1 gene, the host species distribution of V2-TA and V4 profiles was quite wide, since the first was detected in corn, grapevine, potato, tomato, parsley, and parsnip; while the latter was identified in bindweed, carrot, grapevine, potato, tomato, parsley, periwinkle, parsnip, tobacco, and valerian. The vmp1 V4 profile was detected in grapevine samples from Italy, Croatia, Serbia, and Bosnia and Herzegovina [11,27,29,[47][48][49]. On the contrary, the V3 profile showed a host species distribution limited to grapevine, H. obsoletus, P. quinquefolia, and P. tricuspidata from Italy, and it was detected in all the strains tuf type a, only in Italian samples.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the use of several molecular markers suggest a possibility to differentiate BN strains for their differential virulence in grapevine plants [17,26]. However, in most cases molecular markers are mainly used in combination to resolve epidemic cycles at a regional level [11,20,21,24,[27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Molecular Variability and Host Distribution of ‘Candidatus Phytoplasma solani’ Strains from Different Geographic Origins

et al. 2021

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The knowledge of phytoplasma genetic variability is a tool to study their epidemiology and to implement an effective monitoring and management of their associated diseases. ‘Candidatus Phytoplasma solani’ is associated with “bois noir” disease in grapevines, and yellowing and decline symptoms in many plant species, causing serious damages during the epidemic outbreaks. The epidemiology of the diseases associated with this phytoplasma is complex and related to numerous factors, such as interactions of the host plant and insect vectors and spreading through infected plant propagation material. The genetic variability of ‘Ca. P. solani’ strains in different host species and in different geographic areas during the last two decades was studied by RFLP analyses coupled with sequencing on vmp1, stamp, and tuf genes. A total of 119 strains were examined, 25 molecular variants were identified, and the variability of the studied genes was linked to both geographic distribution and year of infection. The crucial question in ‘Ca. P. solani’ epidemiology is to trace back the epidemic cycle of the infections. This study presents some relevant features about differential strain distribution useful for disease monitoring and forecasting, illustrating and comparing the phytoplasma molecular variants identified in various regions, host species, and time periods.

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Vmp1 and stamp genes variability of ‘Candidatus phytoplasma solani’ in Bosnian and Herzegovinian grapevine

Cited by 13 publications

References 12 publications

Epidemiological Investigations and Molecular Characterization of ‘Candidatus Phytoplasma solani’ in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily (Southern Italy)

Epidemiological Investigations and Molecular Characterization of ‘Candidatus Phytoplasma solani’ in Grapevines, Weeds, Vectors and Putative Vectors in Western Sicily (Southern Italy)

Multilocus Genotyping of ‘Candidatus Phytoplasma Solani’ Associated with Grapevine Bois Noir in Iran

Molecular Variability and Host Distribution of ‘Candidatus Phytoplasma solani’ Strains from Different Geographic Origins

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