Vivax malaria detection using a parasitic red blood cell flag generated by the Sysmex XN‐9000 hematology analyzer

Huh, Hee Jin; Chung, Jae-Woo; Park, Seong Yeon; Chae, Seok-Lae

doi:10.1111/ijlh.13672

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…In addition, the gated events showed a correlation with parasite load, demonstrating another function of the Sysmex analyzer. In a similar work, Huh et al focused on P. vivax cases utilizing the XN-9000 analyzer, commonly found in large hospitals with a heavy CBC workload [19]. In our study, we assessed both high-and low-throughput instruments, namely, the XN and its counterpart, the XN-L, using a paired sample approach to establish the clinical relevance and provide direct comparisons.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Plasmodium vivax by the Hematology Analyzer for Population Screening

Khodaiji,

Sehgal,

Sethi

et al. 2023

Diagnostics

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In India, where malaria is endemic, the prompt and accurate detection of infections is crucial for disease management and vector control. Our study aimed to evaluate the “iRBC” flag, a novel parameter developed for routine hematology analyzers, for its sensitivity and specificity in detecting Plasmodium vivax (P. vivax) infections. We used residual blood samples from patients with suspected malaria and compared the iRBC flag results with microscopy, which serves as the gold standard. Additionally, we compared the results with rapid immuno-chromatographic tests (RDTs) commonly used in the field. Our study included 575 samples, of which 187 were positive for P. vivax. The iRBC flag demonstrated a high sensitivity of 88.7% and 86.1% on the XN and XN-L hematology analyzers, respectively, and a clinical specificity of 100% on both analyzers. Furthermore, the scattergram derived from each positive dataset exhibited distinct patterns, which facilitated rapid confirmation by laboratory specialists. Notably, the iRBC flag remained effective even in the presence of interfering conditions. Overall, our results indicate that the iRBC flag is a reliable and rapid screening tool for identifying P. vivax in routine blood testing. Our findings have significant implications for malaria detection and control in endemic regions like India.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection of Plasmodium vivax by the Hematology Analyzer for Population Screening

Khodaiji,

Sehgal,

Sethi

et al. 2023

Diagnostics

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show abstract

“…This prospective, cross-sectional, monocentric, non-interventional study, was conducted in Lyon University Hospital over a period of 12 months. The Standards for Reporting Diagnostic accuracy studies (STARD 2015) hosted by the Enhancing the Quality and Transparency of Health Research (EQUATOR) network were used as methodological support [ 16 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…Together with a complete blood count (CBC), it gives a qualitative result as well as quantification of MI-RBC per microliter (μL), and as a percentage of infected red blood cells (MI-RBC%). This analyser thereby allows for accurate and standardised detection of malaria parasites in human blood as it provides reliable results 24/7, regardless of the availability of a malaria-experienced professional [ 17 , 22 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting

et al. 2022

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Malaria diagnosis based on microscopy is impaired by the gradual disappearance of experienced microscopists in non-endemic areas. Aside from the conventional diagnostic methods, fluorescence flow cytometry technology using Sysmex XN-31, an automated haematology analyser, has been registered to support malaria diagnosis. The aim of this prospective, monocentric, non-interventional study was to evaluate the diagnostic accuracy of the XN-31 for the initial diagnosis or follow-up of imported malaria cases compared to the reference malaria tests including microscopy, loop mediated isothermal amplification, and rapid diagnostic tests. Over a one-year period, 357 blood samples were analysed, including 248 negative and 109 positive malaria samples. Compared to microscopy, XN-31 showed sensitivity of 100% (95% CI: 97.13–100) and specificity of 98.39% (95% CI: 95.56–100) for the initial diagnosis of imported malaria cases. Moreover, it provided accurate species identification asfalciparumor non-falciparumand parasitaemia determination in a very short time compared to other methods. We also demonstrated that XN-31 was a reliable method for patient follow-up on days 3, 7, and 28. Malaria diagnosis can be improved in non-endemic areas by the use of dedicated haematology analysers coupled with standard microscopy or other methods in development, such as artificial intelligence for blood slide reading. Given that XN-31 provided an accurate diagnosis in 1 min, it may reduce the time interval before treatment and thus improve the outcome of patient who have malaria.

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Possible transmission of Plasmodium malariae through liver transplantation: Diagnosis with a Sysmex XN-20™ hematology analyzer

Martínez-Cerezuela,

Tofan,

Sahuquillo-Arce

et al. 2024

Parasitology International

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Vivax malaria detection using a parasitic red blood cell flag generated by the Sysmex XN‐9000 hematology analyzer

Cited by 4 publications

References 13 publications

Rapid Detection of Plasmodium vivax by the Hematology Analyzer for Population Screening

Rapid Detection of Plasmodium vivax by the Hematology Analyzer for Population Screening

Diagnostic accuracy of fluorescence flow-cytometry technology using Sysmex XN-31 for imported malaria in a non-endemic setting

Possible transmission of Plasmodium malariae through liver transplantation: Diagnosis with a Sysmex XN-20™ hematology analyzer

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