Vitrified human ovaries have fewer primordial follicles and produce less antimüllerian hormone than slow-frozen ovaries

Öktem, Özgür; Alper, Ebru; Balaban, Başak; Palaoğlu, Erhan; Peker, Kamil; Karakaya, Cengiz; Urman, Bülent

doi:10.1016/j.fertnstert.2010.12.057

Cited by 59 publications

(47 citation statements)

References 24 publications

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“…In previous contradictory, studies, vitrification has been shown to cause irreversible follicular damage, resulting in an inability to grow during in vitro culture (Oktem et al 2011), while in another study (Isachenko et al 2006), follicular morphology has been preserved after vitrification. Our results are promising, and could be related to a rich culture medium used, supplemented with LIF and KL, among other additives.…”

Section: Discussionmentioning

confidence: 96%

Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture

et al. 2015

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The risk of reintroducing malignant cells after ovarian graft into patients following post-cancer treatment is an obstacle for clinical applications (autotransplantation). In this context, in vitro follicle culture would be an alternative to transplantation in order to minimize such risks. Therefore, the aim of this study was to compare the development of secondary follicles after vitrification in isolated form (without stroma) with vitrification in in situ form (within fragments of ovarian tissue). Follicles were first isolated from ovarian fragments from mixed-breed ewes and then vitrified; these comprised the Follicle-Vitrification group (Follicle-Vit), or fragments of ovarian tissue were first vitrified, followed by isolation of the follicles, resulting in the Tissue-Vitrification group (Tissue-Vit). Control and vitrified groups were submitted to in vitro culture (6 days) and follicular morphology, viability, antrum formation, follicle and oocyte diameter, growth rate, ultrastructural characteristics and cell proliferation were evaluated. The percentages of morphologically normal follicles and antrum formation were similar among groups. Follicular viability and oocyte diameter were similar between Follicle-Vit and Tissue-Vit. The follicular diameter and growth rate of Follicle-Vit were similar to the Control, while those of Tissue-Vit were significantly lower compared to the Control. Both vitrified groups had an augmented rate of granulosa cellular proliferation compared to Control. Secondary follicles can be successfully vitrified before or after isolation from the ovarian tissue without impairing their ability to survive and grow during in vitro culture.

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Section: Discussionmentioning

confidence: 96%

Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture

et al. 2015

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“…One study found that the type of cryoprotectant used affects the results, with propanediol increasing the activation rate following grafting more than with DMSO [37]. Oktem et al [38] have found decreased concentrations of anti-Mϋllerian hormone, known to maintain primordial follicles quiescent, in vitrified ovaries compared to fresh controls following a 3-day culture, as well as a lower primordial follicle count. Others have found no differences in the proportions of primordial follicles in vitrified and control ovarian tissue cultured for 5 days in two different media [12].…”

Section: Discussionmentioning

confidence: 99%

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Mouttham

Fortune

Comizzoli

2015

J Assist Reprod Genet

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Purpose The objective of this study is to characterize the impact of exposure to cryoprotectants followed by vitrification on primordial follicle survival and activation using a fetal bovine model. Methods In the first study, fetal bovine cortical pieces were exposed to cryoprotectants with or without sucrose and cultured up to 7 days in the presence or absence of insulin. In the second study, cortical pieces were exposed to cryoprotectants with or without sucrose, vitrified, and cultured up to 7 days after warming in the presence or absence of insulin. Viability and morphology of follicles, as well as proliferation and/or DNA repair in ovarian tissue were analyzed.Results When compared to non-exposed controls, normal follicular morphology was affected in groups exposed to cryoprotectants only immediately post-exposure and after 1 day of culture, but improved by day 3 and did not significantly differ by day 7. Similarly, normal follicular morphology was compromised in vitrified groups after warming and on day 1 compared to controls, but improved by days 3 and 7. Proliferation and/or DNA repair in ovarian tissue was not affected by vitrification in this model. Cryoprotectant exposure and vitrification of ovarian tissue did not impair the activation of primordial follicles in response to insulin, although activation was delayed relative to non-exposed controls. Interestingly, sucrose had no noticeable protective effect. Conclusion Vitrified fetal bovine ovarian tissue has the intrinsic capacity to mitigate the immediate damage to primordial follicles' morphology and retains the capacity to activate. These findings provide a basis for a successful cryopreservation protocol for ovarian cortical tissue in other species including humans.

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“…In other experiments on vitrification of whole ovaries in the sheep (41) and cow (42), some success have been reported. However, with human ovarian strips, the efficacy of slow-freezing versus vitrification is controversial (43,44). It is also known that the toxicity of DMSO can be reduced by addition of nonpermeable cryoprotectants (45).…”

Section: Discussionmentioning

confidence: 99%

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

Milenković

Díaz-García

Wallin

et al. 2012

Fertility and Sterility

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Vitrified human ovaries have fewer primordial follicles and produce less antimüllerian hormone than slow-frozen ovaries

Cited by 59 publications

References 24 publications

Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture

Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Viability and function of the cryopreserved whole rat ovary: comparison between slow-freezing and vitrification

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