Vitrification solution containing DMSO and EG can induce parthenogenetic activation of in vitro matured ovine oocytes and decrease sperm penetration

Tian, Shujun; Yan, Chao; Yang, Hongwei; Zhou, Guangbin; Yang, Zidan; Zhu, Shi-En

doi:10.1016/j.anireprosci.2007.01.007

Cited by 56 publications

(37 citation statements)

References 7 publications

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“…This rise in calcium level can negatively affect several physiologic processes within oocytes and cause activation. Treatment of oocytes with DMSO or EG has also been shown to cause parthenogenetic activation in sheep (38). However, in the present study parthenogenetic activation of vitrified-warmed oocytes were probably not caused by the cryoprotectants, because the proportions of male pronucleus-and female pronucleus-containing oocytes were nearly the same in the TC group.…”

Section: Discussioncontrasting

confidence: 72%

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization

Gupta

Uhm

Lee

2010

Fertility and Sterility

157

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Section: Discussioncontrasting

confidence: 72%

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization

Gupta

Uhm

Lee

2010

Fertility and Sterility

157

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“…The high survival rates and normal morphology of the vitrified-thawed bovine oocytes could have been achieved due to high rates of cooling and warming. Previous reports have shown that treatment with CPAs or cooling of M-II oocytes may trigger biochemical reactions similar to those phenomena occurring during fertilization, which can result in hardening of the zona pellucida and/or parthenogenetic activation of the oocyte in several mammalian species [12,13,[32][33][34][35]. However, we observed similar fertilization, monospermy and pronuclear formation rates among the Fresh control, Solution control, SSV and Cryotop groups, suggesting that the Solution control treatment, both cooling methods and the warming regimen used in the present study did not cause such phenomena in M-II bovine oocytes and allowed their normal fertilization by IVF.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes

Sripunya

Somfai

Inaba

et al. 2010

Journal of Reproduction and Development

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Abstract. The aim of the present study was to compare the efficacies of the cooling systems of the solid surface (SSV) and Cryotop vitrification methods for cryopreservation of bovine oocytes at the metaphase II stage. The effects of vitrification on oocyte viability, in vitro fertilization (IVF), pronucleus formation and subsequent in vitro development were assessed. In vitro matured (IVM) bovine oocytes were subjected to equilibration and vitrification solutions according to the SSV method, and then the oocytes were vitrified either by dropping onto a cold dry metal surface (SSV group) or by plunging into liquid nitrogen on Cryotop sheets (Cryotop group). Warming was conducted according to the SSV method. Some oocytes were subjected to the cryoprotectants and warming regimen without cooling (Solution control group). The live/dead status of oocytes was evaluated by fluorescein diacetate staining. Live oocytes were subjected to IVF, and the resultant embryos were cultured in vitro. The rates of live oocytes were similar among the Fresh control, Solution control, SSV and Cryotop groups. There was no difference in the rates of fertilization, pronuclear formation and monospermy among these groups. The cleavage rates in the SSV and Cryotop groups (41.6 and 53.2%, respectively) were significantly lower than those in the Fresh control and Solution control groups (65.9 and 61.3%, respectively). The blastocyst rates in SSV and Cryotop groups did not differ (10.3 and 12.8%, respectively); however, they were significantly lower than those in the Fresh control and Solution control groups (36.4 and 24.8%, respectively). The inner cell mass, trophectoderm and total cell numbers in blastocysts did not differ significantly among the Fresh control, Solution control, SSV and Cryotop groups. Our results indicate that IVM bovine oocytes could be cryopreserved successfully using the cooling systems of the Cryotop and Solid Surface Vitrification methods with similar efficacy. Key words: Bovine, Cryotop, Oocyte, Solid surface vitrification, Vitrification (J. Reprod. Dev. 56: [176][177][178][179][180][181] 2010) ryopreservation of bovine oocytes is of great importance in the preservation of female genetic resources. Cryopreserved oocytes can be used for in vitro fertilization (IVF), nuclear transfer and genetic manipulation. Rall and Fahy [1] were the first to report vitrification, a glass-like solidification, by ultra-rapid cooling of mouse embryos, showing an alternative to traditional freezing methods to avoid chilling injury and ice crystal formation. Vitrification has been widely applied to cryopreserve oocytes in other mammalian species including cattle [2] In general, oocytes are more susceptible to cooling damage than zygotes because metaphase spindle microtubule integrity is disrupted during cooling and the high concentration of cryoprotectants affects oocytes during equilibration [10]. Also, differences in the membrane structures seem to make oocytes more sensitive to chilling compared with zygotes [11]. Although the tox...

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“…Glucose, sucrose, fructose, and trehalose are known as extracellular cryoprotectants and they show their effects by increasing the surface area with the interaction of phospholipids that can cause membrane damage during freezing and warming (18,19). Frozen and thawed embryo survival and pregnancy rates after embryo transfer with vitrification methods are more commonly recommended by researchers (20,21).…”

Section: Introductionmentioning

confidence: 99%

Vitrification of in vitro-produced bovine embryos matured inmodified TCM-199 medium

Sandal¹,

Özdaş²

2015

Turk J Vet Anim Sci

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In vitro-produced bovine embryos matured in modified Tissue Culture Medium 199 were vitrified at the 7th and 8th days of culture, and development at 48 h after warming was recorded. Ovaries were obtained from a local abattoir, and the oocytes were recovered by slicing method and divided into two main groups. The first group included cysteamine in the TCM-199 (Group A) and the second did not (Group B). They were incubated for 24 h at 38.8 °C in an atmosphere of 5% CO 2 in humidified air. Matured oocytes were fertilized with 1-2 × 10 6 /mL of frozen-thawed bull semen. At the end of the period the embryos were divided into two subgroups in order to be developed. Therefore, four groups were established: Group AI-SOF (synthetic oviduct fluid), Group AII-CR1aa (Charles Rosencrans), Group BI-SOF, and Group BII-CR1aa. The rates of blastocyst development and expanded blastocysts were detected as 20%, 18.1%, 24.6%, and 22.5% and survival rate as 5.5%, 23.6%, 0%, and 5.2%, respectively, showing statistically significant superiority in Group AII-CR1aa at the level of P < 0.05. In conclusion, supplementing cysteamine into maturation media with subsequent culture in AII-CR1aa had a positive effect on blastocyst survival after vitrification.

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Vitrification solution containing DMSO and EG can induce parthenogenetic activation of in vitro matured ovine oocytes and decrease sperm penetration

Cited by 56 publications

References 7 publications

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization

Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization

A Comparison of Cryotop and Solid Surface Vitrification Methods for the Cryopreservation of In Vitro Matured Bovine Oocytes

Vitrification of in vitro-produced bovine embryos matured inmodified TCM-199 medium

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