Vitrification of oocytes from endangered Mexican gray wolves (Canis lupus baileyi)

Boutelle, Sally; Lenahan, K.; Krisher, Rebecca L.; Bauman, Karen; Asa, Cheryl; Silber, Sherman J.

doi:10.1016/j.theriogenology.2010.10.004

Cited by 24 publications

(15 citation statements)

References 23 publications

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“…Recently, Walter et al (2011) reported that Beagle bitches with deslorelin (4.7 mg; Suprelorin TM ) induced oestrous cycles had significantly fewer oocytes or embryos (3.4 vs 5.0) in uterine horn flushings 9-19 days after ovulation compared with spontaneously cycling bitches. Conversely, in two female Mexican grey wolves stimulated with Ovuplant ® , more oocytes were retrieved following ovarian aspiration compared with those who had not being stimulated (Boutelle et al 2011). Combined, these other studies suggest that the cause for the reduction in litter size is not from a reduction in ovulation rate but rather a reduction in conception rate or higher early embryonic loss rate.…”

Section: Discussionmentioning

confidence: 89%

“…Conversely, in two female Mexican grey wolves stimulated with Ovuplant ® , more oocytes were retrieved following ovarian aspiration compared with those who had not being stimulated (Boutelle et al. ). Combined, these other studies suggest that the cause for the reduction in litter size is not from a reduction in ovulation rate but rather a reduction in conception rate or higher early embryonic loss rate.…”

Section: Discussionmentioning

confidence: 99%

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Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant^®) in Dogs

Wolf

Meyer

Kutzler

2012

Reprod Domestic Animals

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Contents Four German Shorthair Pointer bitches each produced from two to five (total of 14) purebred litters in response to natural matings to either natural oestrus (n = 8) or oestrus induced by Ovuplant®, a sustained‐release implant containing 2.1 mg deslorelin (n = 6). All bitches initially produced litters from natural oestrous matings prior to producing litters from induced oestrus, and two of the bitches also produced natural litters subsequent to their Ovuplant® litters. Mean litter size was lower for Ovuplant® litters (5.4 vs 8.6 puppies; p < 0.001) and within each bitch every induced litter was smaller than any of her natural litters.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant^®) in Dogs

Wolf

Meyer

Kutzler

2012

Reprod Domestic Animals

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“…Inter-species pregnancies have been published for some species of wild ungulates, such as Spanish ibex [14], Texas red sheep [21] and mouflons [22]. However, no report has been published for inter-species embryo transfer of ungulates species with different numbers of chromosomes in embryo and receptor, which is the first obstacle to overcome for aoudad inter-species pregnancies to sheep or goats.…”

Section: Discussionmentioning

confidence: 99%

Superovulation, in vivo Embryo Recovery and Cryopreservation for Aoudad (Ammotragus Lervia) Females Using Osmotic Pumps and Vitrification: A Preliminary Experience and its Implications for Conservation

López-Saucedo

Ramón-Ugalde

Barroso-Padilla³

et al. 2013

Tropical Conservation Science

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The aoudad (Ammotragus lervia) is a wild ruminant considered the living ancestor of domestic sheep and goats. The original distribution of aoudads includes several countries in the North of Africa, but it has been introduced, for trophy-hunting purposes, into other countries (USA, Mexico and Spain).The species was declared vulnerable in the 2012 IUCN's Red List and is also included in the CITES II Appendix. Surprisingly, little is known about its conservation status or the reproductive biology of the natural populations. There are some reports of the application of basic assisted-reproduction techniques in captive aoudads. In this preliminary study, we explored the feasibility of implementing assisted reproduction procedures in captive aoudad females using non-traditional techniques for in vivo embryo production. This approach was used to obtain the best results using the minimum possible number of animals. Three aoudad females were synchronized using a domestic sheep protocol and subcutaneous osmotic pumps for the delivery of follicle-stimulating hormone. A mini-surgical approach combined with laparoscopy was performed to obtain in vivo-produced embryos. All females had an ovulatory response of more than three corporea lutea, but only five good quality morulae were obtained from one female. Those were cryopreserved by vitrification using a Cryotop®. In conclusion, our approach was successful in obtaining in vivo embryos using a limited number of females. Further studies are necessary to optimize the fertilization rate and clarify the effect of our protocol on embryo implantation and the production of offspring.Keywords: Aoudad; Cryotop®; osmotic pumps; superovulation; vitrification, wild ruminant. ResumenAmmotragus lervia, aoudad, es un rumiante silvestre considerando el ancestro de los borregos y cabras domésticas. La distribución original de los aoudad incluye varios países en el Norte de África, pero ha sido introducido, con propósitos de cacería en otros países (EUA, México y España). La especie se declara como vulnerable en la Red List 2012 de la IUCN y también se incluye en el Apéndice II del CITES. Sorprendemente, poco se sabe del estado de conservación o de la biología reproductiva de las poblaciones naturales. Existen algunos reportes de la aplicación de técnicas básicas de reproducción asistida en hembras de aoudad captivas. En este estudio preliminar, exploramos la factibilidad de implementar procedimientos de reproducción asistida en hembras de aoudad captivas usando técnicas no tradicionales para la producción de embriones in vivo. Este enfoque fue utilizado para obtener los mejores resultados utilizando el menor número posible de animales. Tres hembras de aoudad fueron sincronizadas usando un protocolo de borregas domésticas y bombas subcutáneas para la administración de la hormona folículo estimulante. Un abordaje mini-quirúrgico combinado con laparoscopia fue realizado para obtener los embriones producidos in vivo. Todas las hembras tuvieron una respuesta ovulatoria de más de tres cuerp...

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“…While significant improvements have been made in human MII oocyte vitrification these methods have had only a limited impact on oocyte banking in animals over and above the laboratory and livestock species that are used as models for the development and testing of the human ART protocols . Never-the-less encouraging results for MII vitrification have been obtained in some domestic species such as the cat (Fernandez-Gonzalez and Jewgenow, 2017) as well as some exotic species including for example the Tasmanian devil (Czarny and Roger, 2010) and Mexican gray wolf (Boutelle et al, 2011). While commonalities in the biophysical traits associated with cell freezing between taxonomically-related species may be useful to inform protocol development and support improved post-thaw viability across species, in general the similarities between taxa tend to be overshadowed by the magnitude of species specific differences in the ability of their oocytes to tolerate osmotic and toxic effects of CPA exposure and chilling injury (Woods et al, 2004).…”

Section: Cryobiology and Fertility Preservationmentioning

confidence: 99%

Preservation of female fertility in humans and animal species

Picton¹

2018

Anim Reprod

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A detailed understanding of the cryobiology of gametes and complex tissues has led to the development of methods that facilitate the successful low temperature banking of isolated mature human oocytes, or immature oocytes in situ within fragments of human ovarian cortex. Although many outstanding research challenges remain to be addressed, the successful development of new treatments to preserve female fertility for a range of clinical indications has largely been underpinned by the conduct of extensive, fundamental research on oocytes and ovarian tissues from a number of laboratory and commercially important farm species. Indeed, the most recent evidence from large animals suggests that it is also possible to cryopreserve intact whole ovaries along with their supporting vasculature for later autotransplantation and restoration of natural fertility. This review will explore how the methods developed to preserve human oocytes and ovarian tissues can now be used strategically to support the development of conservation strategies aimed at safeguarding the genetic diversity of commercially important domestic animals and also of preserving the female germplasm for wild animals and endangered species.

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Vitrification of oocytes from endangered Mexican gray wolves (Canis lupus baileyi)

Cited by 24 publications

References 23 publications

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant^®) in Dogs

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant^®) in Dogs

Superovulation, in vivo Embryo Recovery and Cryopreservation for Aoudad (Ammotragus Lervia) Females Using Osmotic Pumps and Vitrification: A Preliminary Experience and its Implications for Conservation

Preservation of female fertility in humans and animal species

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Vitrification of oocytes from endangered Mexican gray wolves (Canis lupus baileyi)

Cited by 24 publications

References 23 publications

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant®) in Dogs

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant®) in Dogs

Superovulation, in vivo Embryo Recovery and Cryopreservation for Aoudad (Ammotragus Lervia) Females Using Osmotic Pumps and Vitrification: A Preliminary Experience and its Implications for Conservation

Preservation of female fertility in humans and animal species

Contact Info

Product

Resources

About

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant^®) in Dogs

Litter Size Response to Oestrous Induction with Deslorelin (Ovuplant^®) in Dogs