Vitrification of day 3 cleavage-stage embryos yields better clinical outcome in comparison with vitrification of day 2 cleavage-stage embryos

Chi, Fengli; Luo, Chengfeng; Yin, Ping; Li, Hong; Ruan, Jianbin; Huang, Minghui; Duan, Tao; Tong, Guoqing

doi:10.1017/s0967199413000373

Cited by 8 publications

(7 citation statements)

References 26 publications

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“…They concluded that this strategy could increase the possibility of obtaining more high quality with better developmental potential for vitrification. Their results showed that day 3 embryos vitrification did not affect survival rate, but yielded better clinical outcomes compared to day 2 embryos, especially in poor responder patients (6). Meng et al expressed warmed day 3 embryos that compacted after overnight culture had significantly more chance for live birth (17).…”

Section: Discussionmentioning

confidence: 99%

“…The success rate of FET cycles depends on several factors. Chi and associates demonstrated that 3 days embryos have higher clinical pregnancy rates in poor responders compared to 2 days embryos, although, these results were not different in normal responders (6). Endometrial preparation is another key factor that effect on FET results (7, 8).…”

Section: Introductionmentioning

confidence: 95%

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Does Overnight Culture of Cleaved Embryos Improve Pregnancy Rate in Vitrified-Warmed Embryo Transfer Programme?

Agha‐Rahimi

Omidi

Akyash

et al. 2019

MJMS

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Background Vitrification is a routine procedure in assisted reproductive technique (ART) lab. However, there is widespread variability between protocols of different centres. The aim of this study was to compare the chemical pregnancy, clinical pregnancy and live birth rates between one-day embryo culture and immediate transfer for frozen-thawed embryo transfer (FET) cycles. Methods In this cohort retrospective study, 366 FET cycles were divided into two groups: Group A, the embryos were warmed one day before transfer, and were cultured overnight; Group B, the embryos were warmed on the same day of transfer, at least were cultured 1 h before embryo transfer (ET). Chemical and clinical pregnancy and live birth rates were compared between two groups. Results The chemical pregnancy was higher in group A than B (37.9% versus 28.9%), but this difference was not significant ( P = 0.07). Clinical pregnancy (30.8% versus 24.1%) and live birth (19.8% versus 22.05%) were similar in group A and B, ( P = 0.15), and ( P = 0.8). Conclusion : In conclusion, overnight culture and confirmation of mitosis resumption was not essential for FET cycles in vitrification method.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Does Overnight Culture of Cleaved Embryos Improve Pregnancy Rate in Vitrified-Warmed Embryo Transfer Programme?

Agha‐Rahimi

Omidi

Akyash

et al. 2019

MJMS

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“…A recent study also showed that for each additional embryo biopsied, there is a 13% higher chance of a live birth (Scriven et al , 2013). Therefore, for patients with low number of embryos available for PGD, serial cycles of ovarian stimulation, ICSI and vitrification procedures should be advised especially as high rates of embryo survival were observed following embryo vitrification (Chi et al , 2013; Van Landuyt et al , 2013). In this study, embryo vitrification did not have an adverse impact on the implantation and pregnancy outcomes and previous studies also showed that transferring a cryopreserved embryo did not impair the neonatal outcome (Liu et al , 2013).…”

Section: Discussionmentioning

confidence: 99%

Number of embryos biopsied as a predictive indicator for the outcome of preimplantation genetic diagnosis by fluorescence in situ hybridisation in translocation cases

Tulay

Gültomruk²,

Fındıklı³

et al. 2015

Zygote

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This study aimed to investigate the optimum number of embryos to be biopsied in order to increase the likelihood of obtaining a balanced/normal embryo following preimplantation genetic diagnosis (PGD) by fluorescence in situ hybridisation (FISH) for translocation carriers. Patients with low number of fertilised oocytes (≤5) or low number of embryos available for PGD (<7) underwent multiple hormonal stimulation cycles and their embryos from each cycle were vitrified and accumulated to obtain at least three embryos for PGD. Fifty-seven PGD cycles were performed for translocation carriers by FISH on day 3 of embryo development. PGD and pregnancy outcomes were examined according to the number of embryos biopsied. The cancellation rates of embryo transfer for the reciprocal translocation carriers were 40% when more than eight embryos were biopsied and it was as high as 78% when low number of embryos (less than nine) were biopsied. For Robertsonian translocation carriers, when more than eight embryos were biopsied, there were no embryo transfer cancellations. This study showed that when there are more than nine embryos biopsied for PGD, the likelihood of obtaining a balanced embryo and positive pregnancy outcome is significantly higher (P < 0.05) in such the overall pregnancy rate was 63% for reciprocal and 86% for Robertsonian carriers. This was reduced to only 7% for reciprocal and 14% for Robertsonian translocation carriers when less than nine embryos were biopsied. One of the limitations of this study was that the analysis was performed by FISH and more studies should investigate the outcomes of embryo accumulation following comprehensive chromosome analysis.

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“…4-cell stage embryos at 44 ± 1 h post insemination, 8-cell stage embryos at 68 ± 1 h post insemination with <10 % fragmentation, stage-specific cell size and no multinucleation. However, in practice, most authors report less strict criteria for embryo selection before cryopreservation [ 4 , 10 , 17 – 27 ]. At present, it is not clear if the stricter embryo selection criteria for cryopreservation, reported in the Alpha consensus, actually result in higher IR compared to clinical practices where less strict criteria are applied.…”

Section: Introductionmentioning

confidence: 99%

Effect of embryo morphology and morphometrics on implantation of vitrified day 3 embryos after warming: a retrospective cohort study

Gallardo

Spiessens

D’Hooghe

et al. 2016

Reprod Biol Endocrinol

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BackgroundCharacteristics routinely used to evaluate embryo quality after thawing include number of blastomeres survived and presence of mitosis resumption after overnight culture. It is unknown to which extent symmetry and fragmentation affect implantation after warming and whether application of stricter criteria either before vitrification or after warming would improve implantation rate (IR) of vitrified/warmed embryos. This study aimed to find new parameters to improve selection criteria for vitrification and for transfer after warming.MethodsFirstly, we evaluated standard morphological characteristics (intact survival, mitosis resumption, number of blastomeres, symmetry and fragmentation) of 986 warmed day 3 embryos and, from a subset of 654, we evaluated morphometric characteristics (fragmentation, symmetry and volume change). Secondly, we tested the hypothesis that IR of day 3 vitrified/warmed embryos is influenced by morphometric characteristics. IR per embryo transferred was calculated using embryos that were transferred in a single embryo transfer (SET) or a double embryo transfer (DET) with either 0 or 100 % implantation (830/986). We investigated the significant differences in IR between the different categories of a specific characteristic. These categories were based on our standard embryo evaluation system. The statistical tests Chi-square, Fisher’s exact or Cochrane-Armitage were used according to the type and/or categories of the variable.ResultsThe 986 embryos were transferred in 671 FET cycles with 16.9 % (167/986) IR. After exclusion of DET with 1 embryo implanted, IR per embryo transferred was 12.4 % (103/830). Embryo symmetry, fragmentation and volume change in vitrified/warmed day 3 embryos were not associated with IR. However, when mitosis resumption was present after overnight culture, intact embryos reached significantly higher IR than non-intact embryos and only when the embryo compacted after overnight culture the number of cells damaged after warming had no effect on IR. Concretely, embryos with 8 cells after warming or >9 cells after overnight culture–including compacted embryos–reached the highest IR (>15 %) while embryos with <6 cells after warming or with ≤6 cells after overnight culture had extremely low IR (<1 %).ConclusionsIR of vitrified embryos is determined by the number of cells lost, by the occurrence of mitosis resumption, and by the specific number of blastomeres present but not by fragmentation, blastomere symmetry or volume change. Unselecting embryos for cryopreservation because of fragmentation >10 % and/or symmetry < 75 % only leads to unwanted loss of embryos with acceptable implantation potential.Trial registrationRetrospectively registered NCT02639715.

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Vitrification of day 3 cleavage-stage embryos yields better clinical outcome in comparison with vitrification of day 2 cleavage-stage embryos

Cited by 8 publications

References 26 publications

Does Overnight Culture of Cleaved Embryos Improve Pregnancy Rate in Vitrified-Warmed Embryo Transfer Programme?

Does Overnight Culture of Cleaved Embryos Improve Pregnancy Rate in Vitrified-Warmed Embryo Transfer Programme?

Number of embryos biopsied as a predictive indicator for the outcome of preimplantation genetic diagnosis by fluorescence in situ hybridisation in translocation cases

Effect of embryo morphology and morphometrics on implantation of vitrified day 3 embryos after warming: a retrospective cohort study

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