Vitreous intercellular adhesion molecule 1 in uveitis complicated by retinal detachment

Webster, Lynne; Stanbury, Rosalyn M; Chignell, A H; Limb, G. Astrid

doi:10.1136/bjo.82.4.438

Cited by 12 publications

(8 citation statements)

References 17 publications

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“…[34][35][36] The additional finding that vitreous levels of sVCAM-1 are similarly elevated is not entirely unexpected, since previous investigations have identified these sCAMs within epiretinal membrane specimens of these proliferative retinal diseases. [15][16][17][18][19] These studies, along with reports of soluble forms of CAMs in other systemic and ocular inflammatory conditions, provided the basis for our study and lend support to its findings.…”

Section: Discussionmentioning

confidence: 91%

“…33 Soluble ICAM-1 levels are elevated in the vitreous of patients with PDR, traumatic PVR, and rhegmatogenous RD complicated by uveitis or PVR. [34][35][36] In the current study, we first determined if soluble VCAM-1 is similarly elevated in the vitreous of patients with retinal detachment resulting from either PVR or PDR. We then sought to correlate the vitreous levels of sICAM-1 and sVCAM-1 with clinical parameters of disease, with particular attention to the presence of active neovascularization in PDR and the likelihood of recurrent proliferative disease and/or RD in either condition.…”

Section: Introductionmentioning

confidence: 99%

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Soluble cellular adhesion molecules in proliferative vitreoretinopathy and proliferative diabetic retinopathy

Barile

Chang

Park

et al. 1999

Current Eye Research

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Soluble forms of ICAM-1 and VCAM-1 are increased in the vitreous cavity of patients with RD due to PDR or PVR, reflecting the inflammatory nature of these conditions and suggesting a possible role for these mediators in the pathogenesis of proliferative retinal disease. The vitreous levels of these sCAMs at the time of surgery may serve as a marker of inflammation, but their specific levels do not predict the likelihood of recurrent proliferation or surgical anatomic success in most cases of PVR and PDR.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Soluble cellular adhesion molecules in proliferative vitreoretinopathy and proliferative diabetic retinopathy

Barile

Chang

Park

et al. 1999

Current Eye Research

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“…Upregulation of sICAM-1 is enhanced by inflammatory cytokines, including tumor necrosis factor alpha (TNFa) and it produces pro-inflammatory effects such as recruited leukocytes into the site of the inflammation [82,83]. High concentrations of sICAM-1 are described in patients in vitreoretinopathy [84,85], in uveitis [86] or in sickle cell retinopathy [87]. Increased expression of sICAM-1 was also observed in ischemia/reperfusion (I/R)-induced injury in several organs, and PACAP treatment partially or totally blocked this cytokine [45,77,78,88].…”

Section: Discussionmentioning

confidence: 99%

Protective Role of Endogenous PACAP in Inflammation-induced Retinal Degeneration

Vaczy

Kovari

Kovács

et al. 2018

CPD

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Purpose. Pituitary adenylate cyclase activating polypeptide (PACAP) is a neuroprotective peptide that has been shown to exert protective effects in different models of neurodegenerative diseases, including retinal degenerations. Data obtained from PACAPdeficient (PACAP KO) mice provide evidence that endogenous PACAP has neuroprotective role in different pathologies. PACAP KO mice show enhanced sensitivity to different insults, such as oxidative stress, hypoxia and inflammation. The aim of the present study was to investigate the protective effects of endogenous PACAP in retinal inflammation. Methods. Endotoxin-caused eye inflammation was induced by intraperitoneal injection of lipopolysaccharide (LPS) in PACAP KO and wild type (Wt) mice. After LPS treatment, retinas were processed for histological examination. To detect the alterations of different proteins and cytokines, immunohistochemical, western blot and cytokine array were used. We also performed dark-adapted electroretinography (ERG) to detect the functional differences. Results. The thickness of nearly all layers was significantly less in LPS-injected PACAP KO mice compared to Wt animals. Increased expression of glial fibrillary acidic protein (GFAP) was induced in Müller glial cells after LPS treatment, which was more intense in PACAP KO mice. The levels of pAkt and pGSK were decreased in PACAP KO group during inflammation. LPS treatment significantly increased cytokines (sICAM-1, JE, TIMP-1) in both treated groups, but it was more expressed in PACAP KO animals. Furthermore, ERG responses were disturbed after LPS injection in PACAP KO mice. Conclusion. Our results showed that endogenous PACAP has a protective role in LPS-caused retinal inflammation.

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“…Bovine ocular fluid (BOF) alone and in combination with sheep plasma and human serum albumin has been evaluated as a serum replacement for different cells [ 28 ]. BOF has several active components that promote cell growth, such as vascular endothelial growth factor [ 29 ], the 21-KDa acidic-Ca-binding protein [ 30 ], insulin like growth factor, hypoxanthine, and fibronectin [ 31 ]; however, it does not support cell growth on its own. In addition, ocular fluid has several proteins, including albumin [ 32 ], which may act as non-permeable CPAs.…”

Section: Introductionmentioning

confidence: 99%

Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media

et al. 2015

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Cryostorage is of immense interest in biomedical research, especially for stem cell-based therapies and fertility preservation. Several protocols have been developed for efficient cryopreservation of cells and tissues, and a combination of dimethyl sulfoxide (DMSO) and fetal bovine serum (FBS) is commonly used. However, there is a need for an alternative to FBS because of ethical reasons, high cost, and risk of contamination with blood-borne diseases. The objective of the present study was to examine the possibility of using buffalo (Bubalus bubalis) ocular fluid (BuOF) to replace FBS in cryomedia. Frozen–thawed cells, which were cryopreserved in a cryomedia with BuOF, were assessed for viability, early and late apoptosis, and proliferation. Three cell lines (CHO, HEK, and C18-4), mouse embryonic stem (mES) cells, and primary cells, such as mouse embryonic fibroblast (MEF) cells, human peripheral blood mononuclear cells (hPBMCs), and mouse bone marrow cells (mBMCs), were cryopreserved in cryomedia containing 10% DMSO (D10) with 20% FBS (D10S20) or D10 with 20% BuOF (D10O20). For all three cell lines and mES cells cryopreserved in either D10S20 or D10O20, thawed cells showed no difference in cell viability or cell recovery. Western blot analysis of frozen–thawed-cultured cells revealed that the expression of Annexin V and proliferating cell nuclear antigen (PCNA) proteins, and the ratio of BAX/BCL2 proteins were similar in all three cell lines, mES cells, and hPBMCs cryopreserved in D10S20 and D10O20. However, initial cell viability, cell recovery after culture, and PCNA expression were significantly lower in MEF cells, and the BAX/BCL2 protein ratio was elevated in mBMCs cryopreserved in D10O20. Biochemical and proteomic analysis of BuOF showed the presence of several components that may have roles in imparting the cryoprotective property of BuOF. These results encourage further research to develop an efficient serum-free cryomedia for several cell types using BuOF.

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Vitreous intercellular adhesion molecule 1 in uveitis complicated by retinal detachment

Cited by 12 publications

References 17 publications

Soluble cellular adhesion molecules in proliferative vitreoretinopathy and proliferative diabetic retinopathy

Soluble cellular adhesion molecules in proliferative vitreoretinopathy and proliferative diabetic retinopathy

Protective Role of Endogenous PACAP in Inflammation-induced Retinal Degeneration

Ocular Fluid As a Replacement for Serum in Cell Cryopreservation Media

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