Vitellogenin-like protein measurement in caged Gammarus fossarum males as a biomarker of endocrine disruptor exposure: Inconclusive experience

Gammarids as Reference Species for Freshwater Monitoring

“…In general, enzymatic biomarkers developed for vertebrates have been transferred to invertebrates without systematic molecular characterization. This methodology has been used to investigate whether VTG measurement could be a specific endocrine disruption biomarker in males and used as an indicator of feminization (Jubeaux et al, 2012c;Short et al, 2014). However, for proteins which sequence and/or physiological function have significantly changed during evolution, this transfer approach leads to inconsistent results in invertebrates.…”

“…In situ assays may be based on life-history traits (survival, growth, and reproduction), physiological measurements (scope for growth, feeding) or molecular markers. In situ assays using gammarids have been used to assess a range of contaminant sources, such as wastewater treatment plants (Lacaze et al, 2011b) and industrial inputs (Dedourge- Geffard et al, 2009) or for large-scale surveys (Coulaud et al, 2011;Jubeaux et al, 2012c). In situ assays using gammarids have been used to assess a range of contaminant sources, such as wastewater treatment plants (Lacaze et al, 2011b) and industrial inputs (Dedourge- Geffard et al, 2009) or for large-scale surveys (Coulaud et al, 2011;Jubeaux et al, 2012c).…”

“…A second series of experiments quantified VTG protein in males deployed in reference and contaminated stations at different seasons (Jubeaux et al, 2012c). Studies on the possible induction of VTG in Gammarus illustrate this problem.…”

Copper sulphate reduces the metabolic activity of Gammarus fossarum in laboratory and field experiments

“…Böttger et al, 2013;Prato et al, 2013;Berghahn et al, 2012;Jubeaux et al, 2012;Bundschuh et al, 2011). In comparison, fewer field studies have been conducted using this taxon (but see e.g.…”

Multiplexed assay for protein quantitation in the invertebrate Gammarus fossarum by liquid chromatography coupled to tandem mass spectrometry

“…We already applied this method to G. fossarum for measuring a key protein involved in reproduction, the vitellogenin [7,8]. SRM, named method of the year 2012 [9], is a hypothesis-driven method offering outstanding multiplexing capabilities, as demonstrated recently for systems biology studies and the clinical evaluation of biomarker candidates [10].…”