Vitellogenin genes in fish: differential expression on exposure to estradiol

Rawat, Varunendra Singh; Rani, Kumari Vandana; Phartyal, Rajendra; Sehgal, Neeta

doi:10.1007/s10695-012-9626-0

Cited by 17 publications

(9 citation statements)

References 46 publications

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“…In order to investigate whether miRNAs play some roles in testis development, we checked the expression of miR-200 family members in testis treated with high dose of estrogen that would impair testis development and cell proliferation [33]. Expression of five miR-200 family members (200a-3p, 200b-3p, 200c-3p, 141-3p, 429b-3p) was significantly up-regulated at 12 h, and were gradually rising to a higher value at 96 h after EE2 treatment compared to the expression of control group (Figure 6).…”

Section: Resultsmentioning

confidence: 99%

“…Based on previous estrogen treatment studies, the dose of EE2 (10 ug/g body weight) was considered as a relative high concentration [33], [52], [53]. Under high dose of EE2 treatment, testis development and spermatogenesis is impaired, including inhibition of spermatogonia proliferation and differentiation, apoptosis of undifferentiated spermatogonia and reduction of seminal fluid volume [31], [54]–[56].…”

Section: Discussionmentioning

confidence: 99%

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Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish

Jing

Liu

et al. 2014

PLoS ONE

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Recently, YY super-male yellow catfish had been created by hormonal-induced sex reversal and sex-linked markers, which provides a promising research model for fish sex differentiation and gonad development, especially for testis development. MicroRNAs (miRNAs) have been revealed to play crucial roles in the gene regulation and gonad development in vertebrates. In this study, three small RNA libraries constructed from gonad tissues of XX female, XY male and YY super-male yellow catfish were sequenced. The sequencing data generated a total of 384 conserved miRNAs and 113 potential novel miRNAs, among which 23, 30 and 14 miRNAs were specifically detected in XX ovary, XY testis, and YY testis, respectively. We observed relative lower expression of several miR-200 family members, including miR-141 and miR-429 in YY testis compared with XY testis. Histological analysis indicated a higher degree of testis maturity in YY super-males compared with XY males, as shown by larger spermatogenic cyst, more spermatids and fewer spermatocytes in the spermatogenic cyst. Moreover, five miR-200 family members were significantly up-regulated in testis when treated by 17α-ethinylestradiol (EE2), high dose of which will impair testis development and cell proliferation. The down-regulation of miR-141 and 429 coincides with the progression of testis development in both yellow catfish and human. At last, the expression pattern of nine arbitrarily selected miRNAs detected by quantitative RT-PCR was consistent with the Solexa sequencing results. Our study provides a comprehensive miRNA transcriptome analysis for gonad of yellow catfish with different sex genotypes, and identifies a number of sex-biased miRNAs, some of that are potentially involved in testis development and spermatogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish

Jing

Liu

et al. 2014

PLoS ONE

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“…Moreover, the injection of adult male Indian freshwater murrel fish (Channa punctatus) with E2 (1, 10 and 40 μg/100g/body wt.) showed a dose-dependent response with respect to VTG genes expression (22). Similarly, Arukwe et al (26) observed a significant increase in VTG gene expression in a dose and time dependent pattern in rainbow trout fish (Oncorhynchus mykiss) when treated with varying doses of nonylphenol.…”

Section: Discussionmentioning

confidence: 86%

“…In this study, we developed a real time PCR assay for VTG gene expression in A. latus. It has been demonstrated that E2, as a natural estrogen, can induce the expression of hepatic VTG gene in various fish (9,22,23).…”

Section: Discussionmentioning

confidence: 99%

Vitellogenin Gene Expression and Sex Steroid Levels as Biomarkers in Yellowfin Seabream (Acanthopagrus latus) Exposed to Bisphenol-A

Negintaji

Safahieh

Zolgharnein

et al. 2019

IJT

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Background: The egg yolk precursor protein vitellogenin (VTG) has proven to be a useful biomarker, used to identify organisms exposed to estrogenic compounds. Methods: We investigated variations in the VTG gene expression pattern and plasma sex steroid hormones concentrations in the yellowfin Seabream, Acanthopagrus latus, (A. latus) by various doses of bisphenol-A (BPA) exposure for 7 and 14 days. We developed a quantitative real time polymerase chain reaction (RT-PCR) assay for the expression of VTG gene in A. latus. The dose-response pattern of VTG gene expression in A. latus exposed to various doses of BPA was characterized. In order to design RT-PCR primers specific to A. latus VTG, a partial sequence of the VTG gene was obtained. Results: The RT-PCR assay was effective in detecting increased VTG gene expression in A. latus exposed to BPA. It also demonstrated that the VTG expression was affected by BPA in a dose and time-dependent manner. Plasma testosterone (T) levels were decreased in the treated fish in comparison with those found in the control group, when they were exposed to 100 µg/g of BPA and 2 µg/g of E2. In contrast, the plasma levels of 17β-estradiol (E2) were significantly increased in a dose-dependent manner. Conclusion: The results suggest that VTG mRNA quantification can provide a sensitive and early signal in the detection of estrogens in marine wildlife. It also indicated that BPA could lead to an imbalance of sex steroid hormones with potentially harmful consequences on sexually immature male A. latus.

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“…In teleosts, the three YPs derived from Vtg (lipovitellin, Lv; phosvitin; and β′-component) play essential roles in embryonic and larval fish nutrition (Hiramatsu, Matsubara, Weber, Sullivan, & Hara, 2002). The most evolved groups of fish (Paracanthopterygii and Acanthopterygii) possess three Vtg orthologues, namely: Vtg type A (VtgA or VtgAa), Vtg type B (VtgB or VtgAb) and Vtg type C (VtgC) (Rawat, Rani, Phartyal, & Sehgal, 2013).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a competitive hybrid ELISA for Seriola lalandi Vitellogenin

Sanchis

Nocillado

Cheetham

et al. 2020

Aquaculture Research

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The main objective of this study was to develop an enzyme-linked immunosorbent assay (ELISA) for Seriola lalandi vitellogenin (Vtg). Nucleotide sequences corresponding to VtgAa, VtgAb and VtgC were obtained from S. lalandi liver transcriptomic data.Two peptides (12 amino acids each) were synthesized according to the predicted conserved regions of VtgAa and VtgAb, and used to generate two polyclonal antibodies in rabbits: anti-S. lalandi VtgA and anti-S. lalandi VtgB. Plasma from male S. lalandi treated with 17β-estradiol (E 2 ) was fractionated to produce the Vtg standard and analysed through SDS-PAGE and Western blot. LC-MS/MS analysis showed that the fraction corresponding to the highest peak consisted mainly of VtgAa and VtgAb.Two homologous antibodies (anti-S. lalandi VtgA and anti-S. lalandi VtgB) and one heterologous antibody (anti-Mugil cephalus Vtg) were tested to develop the S. lalandi ELISA. While the antibodies against the Vtg peptides were not effectively recognizing the Vtg standard, parallelism was confirmed between the M. cephalus Vtg standard and the S. lalandi Vtg standard. The ELISA developed is highly specific (no cross-reaction with other proteins present in plasma when samples were diluted over 1:20), precise (the inter-assay coefficient of variation at 50% binding was 13%) and sensitive (19 ng/ml). The assay was validated by quantifying the plasma Vtg levels in females at the start of the reproductive season and in E 2 -treated males.

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Vitellogenin genes in fish: differential expression on exposure to estradiol

Cited by 17 publications

References 46 publications

Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish

Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish

Vitellogenin Gene Expression and Sex Steroid Levels as Biomarkers in Yellowfin Seabream (Acanthopagrus latus) Exposed to Bisphenol-A

Development and validation of a competitive hybrid ELISA for Seriola lalandi Vitellogenin

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