Vitellogenin gene expression in the intertidal blenny Lipophrys pholis: A new sentinel species for estrogenic chemical pollution monitoring in the European Atlantic coast?

Ferreira, Filipa; Santos, Miguel M.; Castro, L. Filipe C.; Reis-Henriques, M.A.; Lima, Daniela Delwing-de; Vieira, Maria Natividade; Monteiro, Nuno

doi:10.1016/j.cbpc.2008.07.002

Cited by 16 publications

(12 citation statements)

References 58 publications

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“…3). It seems important to stress that, although Cabo do Mundo is referred to as a contaminated site (Ferreira et al 2009) due to the presence of petrochemical industry, no testis-ova were found.…”

Section: Discussionmentioning

confidence: 92%

“…Considering that L. pholis is generally abundant and easy to catch, inhabits the intertidal zone during the majority of its lifecycle while displaying a restricted home range, and exhibits wide geographical distribution, it may be assumed that this blenniid combines some of the basic characters expected in a sentinel species. There is now evidence that L. pholis is also highly responsive to a wide variety of pollutants, from those present in oil spills (Lyons et al 1997;Harvey et al 1999), to neurotoxic compounds (Solé et al 2008), organic contaminants or estrogenic chemicals (Ferreira et al 2009). …”

Section: Introductionmentioning

confidence: 98%

“…The development of biological early warning systems, able to signal the presence of contaminants in aquatic environments, emphasized the need for validation of sentinel species, intrinsically sensitive to chemical pollution. Although several fish species have been increasingly used as sentinels in marine ecosystem biomonitoring programs, some problems often arise due to limitations imposed by the particular ecology of some of the most well-established model species (Ferreira et al 2009). Thus, there is still space for the introduction of previously unused model species whose biology is tightly connected with the environment being assessed.…”

Section: Introductionmentioning

confidence: 99%

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The annual cycle of spermatogenesis in Lipophrys pholis (Blenniidae), a recently proposed sentinel species for pollution monitoring

et al. 2011

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Since reproductive parameters are common endpoints in pollution monitoring, the description of the spermatogenesis of Lipophrys pholis, a recently proposed sentinel species, is of critical importance. Mature males were used to determine the annual maturation cycle, using a stereological approach. The spermatogenic cycle was classified into three stages: early spermatogenesis (May), mid spermatogenesis (September), and spawning (November and January). The testes were asynchronously arranged with various cell types observable during all seasons, although in different proportions. Gonadosomatic index and spermatozoa proportions confirmed the established reproductive season. Differences between the sampled populations and those from septentrional locations are discussed, namely the absence of ''resting'' and ''spent'' periods.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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The annual cycle of spermatogenesis in Lipophrys pholis (Blenniidae), a recently proposed sentinel species for pollution monitoring

et al. 2011

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“…Some fish that have been exposed in single species studies include the blenny Lipophrys pholis (Ferreira et al 2009); brown trout Salmo trutta (Korner et al 2005); guppy Poecilia reticulate (Nielsen & Baatrup 2006); and three-spined stickleback Gasterosteus aculeatus (Bjorkblom et al 2009). When comparing results between these studies, some interspecies differences are noteworthy and include vitellogenin induction in blenny and three-spined stickleback, whereas guppy showed no effects related to the estrogenic exposure at environmentally relevant concentrations (lowest observed-effects concentration was 200 ng/L EE2 [Nielsen and Baatrup 2006]).…”

Section: Discussionmentioning

confidence: 99%

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

Jorgenson

Buhl

Bartell

et al. 2014

Arch Environ Contam Toxicol

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Although the effects of estrogens on model laboratory species are well documented, their utility as surrogates for other species, including those listed as endangered, are less clear. Traditionally, conservation policies are evaluated based on model organism responses but are intended to protect all species in an environment. We tested the hypothesis that the endangered Rio Grande silvery minnow (Hybognathus amarus) is more vulnerable to endocrine disruption-as assessed through its larval predator-escape performance, survival, juvenile sex ratios, and whole-body vitellogenin concentration-than the commonly used toxicological model species fathead minnow (Pimephales promelas) and the bluegill sunfish (Lepomis macrochirus). Fish were exposed concurrently for 21 days to the model endocrine active compound (EAC) 17ß-estradiol (E2) at 10 ng E2/L and 30 ng E2/L in a flow-through system using reconstituted water that simulated the physicochemical conditions of the Middle Rio Grande in New Mexico, USA. No significant differences were observed between the fathead and silvery minnow in larval predator-escape response or juvenile sex ratio. Rio Grande silvery minnow survival decreased significantly at day 14 compared with the other two species; by day 21, both cyprinid species (silvery minnow and fathead minnow) exhibited a significant decrease in survival compared with bluegill sunfish, a member of the family Centrarchidae. Male Rio Grande silvery minnow showed a significant increase in whole-body vitellogenin concentration in the 10 ng/L treatment, whereas fathead minnow and bluegill sunfish showed no significant increases in vitellogenin concentrations across treatments. Our study showed response differences to estrogen exposures between the two cyprinid species and further divergence in responses between the families Cyprinidae and Centrarchidae. These results suggest that commonly used laboratory model organisms may be less sensitive to EACs than the endangered Rio Grande silvery minnow. However, this study supports the continued use of surrogate species for the beneficial implementation of water-quality regulations for the protection of threatened and endangered species if phylogenetic relationships are taken into consideration.

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“…While several studies have been conducted using species as models to assess the negative impacts of endocrine disrupting chemicals, many of the proposed sentinel species have important drawbacks that limit their use or reliability (Schladot et al 1997, Frenzilli et al 2004, Gercken et al 2006, such as a narrow geographical distribution and migratory behaviour. Recently, the intertidal Blenniidae Lipophrys pholis (Linnaeus, 1758) has emerged as a promising sentinel species for monitoring pollution in the northeastern Atlantic and has already proven to be responsive to organic contaminants such as polycyclic hydrocarbons , neurotoxic compounds (Solé et al 2008), oil spills , Lyons et al 1997, Harvey et al 1999, oil shale extracts (Lewis et al 1986) and estrogenic chemicals (Ferreira et al 2009).…”

Section: Introductionmentioning

confidence: 99%

The annual cycle of oogenesis in the shanny, <i>Lipophrys pholis</i> (Pisces: Blenniidae)

Ferreira¹,

Santos²,

Reis-Henriques³

et al. 2012

Sci. Mar.

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SUMMARY:Lipophrys pholis has been shown to be responsive to a variety of environmental contaminants, some of them able to impair reproduction. Description of the normal cycle of oogenesis of this newly proposed sentinel species is important since this data may function as a baseline for comparison in ecotoxicological studies, among other applications. Based on histological observations, L. pholis ovarian development in adult is asynchronous, and 7 ovarian germ cells can be described (oogonia, early and late perinuclear oocytes, cortical-alveolar oocytes, early vitellogenic oocytes, vitellogenic oocytes and spawning oocytes). Using a stereological approach together with the morphologic characteristics of ovarian cells, the ovarian cycle of L. pholis was divided into 3 maturation stages: early oogenesis (May); mid-oogenesis (September), and spawning (November to January). Ovarian cell proportions and gonadosomatic index confirmed that the reproductive period of L. pholis near the southern limit of distribution of the species occurs during cold-water periods, between November and May. The collected data will help to fill some of the gaps in information that still exist on L. pholis oogenesis, thus allowing a better integration of this species as a sentinel for the detection of contaminants in European coastal waters.Keywords: Lipophrys pholis, sentinel species, intertidal, oogenesis, gonadal development. RESUMEN: Ciclo anual de oogénesis en Lipophrys phoLis (Pisces: Blenniidae). -En trabajos recientes, se ha visto que Lipophrys polis es sensible a diversos contaminantes medioambientales, algunos de los cuales pueden afectar a la reproducción. La descripción del ciclo normal de oogénesis de esta nueva propuesta de especie centinela es importante, ya que serviría como referencia para futuros estudios toxicológicos, entre otras aplicaciones. En base a observaciones histológicas, se pueden describir siete estados de los oocitos (oogonia, estado perinuclear temprano y tardío, estado cortico-alveolar, vitelogénesis temprana, vitelogénesis y oocitos desovados). Desde un enfoque estereológico, junto con las características morfológicas de las células ováricas, el ciclo ovárico de L. polis se ha dividido en tres estados de maduración: oogénesis temprana (mayo), oogénesis media (septiembre) y desove (de noviembre a enero). Las proporciones de las células ováricas y el índice gonadosomático, confirmaron que el periodo reproductivo de L. polis, en el límite meridional de distribución de la especie, se produce durante los periodos de agua fría, entre noviembre y mayo. Los datos obtenidos ayudarán a cubrir algunas de las lagunas que todavía existen en el conocimiento de la ovogénesis de L. pholis, permitiendo, por tanto, una mejor integración de esta especie como centinela para la detección de contaminantes en las aguas costeras europeas.

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Vitellogenin gene expression in the intertidal blenny Lipophrys pholis: A new sentinel species for estrogenic chemical pollution monitoring in the European Atlantic coast?

Cited by 16 publications

References 58 publications

The annual cycle of spermatogenesis in Lipophrys pholis (Blenniidae), a recently proposed sentinel species for pollution monitoring

The annual cycle of spermatogenesis in Lipophrys pholis (Blenniidae), a recently proposed sentinel species for pollution monitoring

Do Laboratory Species Protect Endangered Species? Interspecies Variation in Responses to 17β-Estradiol, a Model Endocrine Active Compound

The annual cycle of oogenesis in the shanny, <i>Lipophrys pholis</i> (Pisces: Blenniidae)

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