Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads

Moncaut, Natalia; Nostro, Fabiana Laura Lo; Maggese, M.C.

doi:10.1016/s0166-445x(02)00175-3

Cited by 69 publications

(48 citation statements)

References 36 publications

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“…In accordance with the present study, a previous study by Moncaut et al (2003) has demonstrated that natural E2, in an intraperitoneal injection of 10 μg/g fish weight, induces detectable levels of Vtg in surface mucus of a cichlid fish (Cichlasoma dimerus). The biological significance of the presence of large amounts of these proteins in the surface mucus of fish exposed to estrogenic compounds is presently not known.…”

Section: Discussionsupporting

confidence: 93%

Molecular and cellular detection of expression of vitellogenin and zona radiata protein in liver and skin of juvenile salmon (Salmo salar) exposed to nonylphenol

Arukwe

Røe

2007

Cell Tissue Res

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In developing bioassays for estrogenic effects, vitellogenin (Vtg) induction and zona radiata protein (Zr-protein) induction in males and juveniles of oviparous vertebrates have been used as sensitive biomarkers for estrogenicity. Nonylphenol (NP) produces similar and parallel expression patterns of Vtg and Zr-protein levels in plasma and surface mucus of salmon, the response being concentration- and time-dependent. We have explored the potential mechanisms of Vtg and Zr-protein expression in surface mucus by comparative molecular and cellular approaches. Liver, skin, blood, and surface mucus samples were collected from fish exposed to a single waterborne concentration of NP (10 and 60 microg/l), 3, 7, and 10 days post-exposure, for gene expression analysis (liver and skin; quantitative real-time polymerase chain reaction) and protein analysis (blood and surface mucus; enzyme-linked immunosorbent assay). Protein expression was localized by immunohistochemistry. NP produced concentration- and time-dependent increases of hepatic estrogen receptors (ERalpha and ERbeta), Vtg, and Zr-protein mRNA and plasma protein levels. These responses paralleled cellular detection of Vtg and Zr-protein in the liver with unique expression patterns in the cytoplasm of hepatocytes, hepatic sinusoids, and endothelial cells. ERalpha, Vtg, and Zr-protein mRNA were detectable in the skin. ERbeta was the only skin response that was NP-concentration-dependent, especially at day 10 post-exposure. Immunohistochemistry for Vtg and Zr-protein in skin showed unique expression patterns in mucus vacuoles, epidermal cells, and scales in an NP-concentration- and time-specific manner. Thus, analysis of skin mRNA levels for xenoestrogen biomarker responses is a less-promising approach than protein analysis. The immunohistochemical localization of Vtg and Zr-protein levels in the skin further validates surface mucus as a sensitive biomarker source for estrogenic compounds. These responses represent an improvement for the detection of endocrine-disrupting compounds and related pollutants in the environment.

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Section: Discussionsupporting

confidence: 93%

Molecular and cellular detection of expression of vitellogenin and zona radiata protein in liver and skin of juvenile salmon (Salmo salar) exposed to nonylphenol

Arukwe

Røe

2007

Cell Tissue Res

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“…E2-treated testes revealed severe damage on testicular structure, moreover, decreased sperms were observed significantly in the spermatogenic tubules which revealed disorganization and a premature breakup of spermatocysts (Figure 3(b)). These histopathological changes were also found on other fish models [34,35] . Unexpectedly, in the co-exposed group of QDs and E2 (Figure 3(c) and Figure 3(d)), there were no apparent hypertrophied efferent duct cells in the slices of testes.…”

Section: Histological Observationssupporting

confidence: 74%

Effects of nanoscale quantum dots in male Chinese loaches (Misgurnus anguillicaudatus): Estrogenic interference action, toxicokinetics and oxidative stress

Luo

Tao

et al. 2009

Sci. China Ser. B-Chem.

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“…This steroid hormone induces the biosynthesis of vitellogenin in the liver of mature female fish, which is subsequently incorporated into the developing oocyte (Collier et al, 2003;Swanson et al, 2003;Guerriero, 2007). A correspondence between plasma concentration of E2 and the size or weight of the gonads has been established (Aruke and Goksoyr, 2003;Moncaut et al, 2003;Berg et al, 2004), therefore, it is considered as an indicator of female gonadal maturation or final oocyte maturation in female fish (Scott et al, 1998;Kokokiris et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Fatty acid and proximate composition of wild male and female king angelfish (Holacanthus passer) gonads during the ripe and spent developmental stages

et al. 2016

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Overfishing of king angelfish Holacanthus passer reduced populations in the Gulf of California. This study aimed to determine the fatty acid and proximate composition of male and female gonads, and the E2 concentration in plasma of wild organisms with different stages of gonadal development captured during early and mid-summer at El Datil, near Kino Bay, Sonora, Mexico, to help establish basic reproductive aspects and possibly to help develop the aquaculture of this ornamental species. Out of the 45 adult specimens analyzed, 66.7% were females and 33.3% were males; 16.7% of the females had ripe gonads while 83.3% had spent gonads, and 20% of males had ripe gonads while 80% had spent gonads, indicating they were reproducing earlier, likely in the spring. Females had smaller size but numerically greater gonadosomatic index (GSI) and condition factor (K) than males. Ripe and spent females showed no statistical differences in weight, length or K; however, ripe females showed significantly higher GSI (P = 0.0005) than spent females, 3.43 vs. 0.87%. Lipid content was higher in ripe gonads, with higher content of fatty acids. In both sexes, DHA was quantitatively the major fatty acid, (10.83-16.28 mg of DHA g -1 of gonad wet weight). The n-3/n-6 ratios varied from 1.99 to 3.54, lower for ripe organisms due to a higher content of ARA and n-6 derivatives. Gonad DHA content changed in relation to its developmental stage and it might serve as an additional maturation index. Information on the chemical composition and fatty acid profile of the gonads can be used as indicators of dietary lipid and essential fatty acid requirements of broodfish, which may contribute to the formulation of a balanced diet for the culture of this ornamental species.

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Vitellogenin detection in surface mucus of the South American cichlid fish Cichlasoma dimerus (Heckel, 1840) induced by estradiol-17β. Effects on liver and gonads

Cited by 69 publications

References 36 publications

Molecular and cellular detection of expression of vitellogenin and zona radiata protein in liver and skin of juvenile salmon (Salmo salar) exposed to nonylphenol

Molecular and cellular detection of expression of vitellogenin and zona radiata protein in liver and skin of juvenile salmon (Salmo salar) exposed to nonylphenol

Effects of nanoscale quantum dots in male Chinese loaches (Misgurnus anguillicaudatus): Estrogenic interference action, toxicokinetics and oxidative stress

Fatty acid and proximate composition of wild male and female king angelfish (Holacanthus passer) gonads during the ripe and spent developmental stages

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