Vitellin and vitellogenin in the soldier bug,Podisus maculiventris: Identification with monoclonal antibodies and reproductive response to diet

Shapiro, Jeffrey P.; Wasserman, Heather A.; Greany, P. D.; Nation, James L.

doi:10.1002/1520-6327(200007)44:3<130::aid-arch4>3.0.co;2-b

Cited by 22 publications

(19 citation statements)

References 16 publications

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“…Like for the caterpillar prey tested, oviposition rate of P. maculiventris on a meat-based artificial diet in the present study was inferior to that recorded in previous studies (De Clercq and Degheele, 1992a;De Clercq et al, 1998b). Other workers have also successfully reared P. maculiventris on various artificial diets (e.g., Shapiro et al, 2000;Wittmeyer and Coudron, 2001). Both P. maculiventris and P. bidens have been characterized as polyphagous predators feeding on a wide range of insect prey (see De Clercq, 2000, for a review).…”

Section: Discussionmentioning

confidence: 99%

Effects of diet on development and reproduction of the predatory pentatomids Picromerus bidens and Podisus maculiventris

et al. 2006

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The effects of five diets (larvae of Galleria mellonella L., larvae of Spodoptera littoralis (Boisduval), eggs of Ephestia kuehniella Zeller, cysts of Artemia franciscana Kellogg and an artificial diet based on bovine meat) on development, survival and reproduction of two predatory stinkbugs, Picromerus bidens L. and Podisus maculiventris (Say), were studied in the laboratory. Both species successfully completed immature development on the foods offered, except for P. bidens on cysts of A. franciscana. Significant effects of diet on developmental duration were found in both species. Total developmental time from second instar to adult ranged from 25.0 to 41.5 days and from 18.7 to 46.0 days in P. bidens and P. maculiventris, respectively. Nymphal survival of P. maculiventris was superior to that of P. bidens on all diets tested. Nymphal survival of P. maculiventris was greater than 92% on all diets except on A. franciscana cysts, yielding only 50% survival to adulthood. Survival of P. bidens fed on eggs of E. kuehniella was higher than that of conspecifics fed on caterpillar prey or artificial diet (89% on flour moth eggs vs. 68 and 80% on G. mellonella and S. littoralis and only 50% on meat diet). Fresh weight of newly emerged females of both pentatomids was affected by the diet offered to the predators during their nymphal stage. Females of P. maculiventris produced viable eggs on all diets except on cysts of A. franciscana and had mean fecundities of 691, 436, 608 and 344 eggs per female on S. littoralis, G. mellonella, E. kuehniella eggs and the meat diet, respectively. Females of P. bidens laid eggs only on live prey with mean fecundities of 94 and 38 eggs per female on S. littoralis and G. mellonella, respectively. The results indicate a lower nutritional plasticity of P. bidens as compared with P. maculiventris.

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Section: Discussionmentioning

confidence: 99%

Effects of diet on development and reproduction of the predatory pentatomids Picromerus bidens and Podisus maculiventris

et al. 2006

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“…Vitellogenesis is a major component of the oogenesis process in insects (Shapiro et al, 2000) and it comprises the synthesis of the yolk protein precursor vitellogenin in the fat body, its release into the haemolymph and transport to the follicles, which selectively bind and internalise it into oocytes as vitellin (Raikhel and Dhadialla, 1992;Bitondi and Simões, 1996). Podisus nigrispinus fed on 5th instar cotton leafworm may be able to synthesise larger quantities of vitellogenin, which would explain their higher reproductive rate.…”

Section: Resultsmentioning

confidence: 84%

Diet affects reproduction and number of oocytes per ovary of the predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae)

Lemos¹,

Ramalho²,

Serrão³

et al. 2006

Animal Biol

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The effect of the type of diet consumed on the reproduction of Podisus nigrispinus (Dallas) was studied. Females of P. nigrispinus fed on an artificial diet had a longer preoviposition period than those fed on 3rd or 5th instar cotton leafworm larvae [Alabama argillacea (Hübner)] or mealworm (Tenebrio molitor L.) larvae. P. nigrispinus females fed on 3rd or 5th instar cotton leafworm larvae or mealworm larvae had a longer oviposition period than females on an artificial diet. The numbers of egg masses per female and of egg masses per female per day were higher when P. nigrispinus was fed on 3rd or 5th instar cotton leafworm larvae than when fed on an artificial diet. Podisus nigrispinus females fed on 5th instar cotton leafworm larvae produced a greater number of eggs per female, eggs per egg mass, and eggs per female per day, had a higher nymphal hatch rate than those fed other diets and had higher numbers of oocytes per central ovariole or per ovary than those fed on 3rd instar cotton leafworm larvae, artificial diet, house fly (Musca domestica L.) larvae or mealworm larvae.

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“…The amount of vitellin and vitellogenin can be a measure of reproductive fitness and a potential predictor of fecundity. Some studies have used enzyme-linked immunosorbent assays (ELISA) to determine vitellin and vitellogenin contents in females, such as for the parasitoid Pteromalus puparum (Hymenoptera: Pteromalidae) (Dong et al 2007), the haematophagous insect Dipetalogaster maxima (Heteroptera: Reduviidae) (Aguirre et al 2008), and the heteropterans Podisus maculiventris and Orius insidiosus (Shapiro et al 2000;Shapiro and Ferkovich 2002).…”

Section: Introductionmentioning

confidence: 84%

Egg detection in females of the polyphagous predator Macrolophus pygmaeus (Heteroptera: Miridae) by serological techniques

et al. 2010

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Macrolophus pygmaeus is a polyphagous mirid bug native to the Mediterranean area where it is widely used in the biological control of whiteflies and other pests in vegetable crops. To assess the presence of eggs in females is important in order to optimize predator release strategies, as well as their mass rearing. This study shows an alternative to the traditional procedures of fertility assessment based on evaluation of newly hatched nymphs. A polyclonal antiserum was produced against M. pygmaeus egg proteins, and an ELISA protocol was developed to determine whether laboratory-reared or field-collected females contained egg proteins (mainly vitellin). When field-collected females were analysed, an 80% contained egg proteins indicating that they were reproductively active. Presence/absence of egg proteins was detected with an error than 1%. Since this ELISA protocol allowed also the detection of egg protein precursors, it was more precise and less time consuming than conventional techniques as abdomen dissection or egg-hatch evaluation in determining egg presence in females.

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Vitellin and vitellogenin in the soldier bug,Podisus maculiventris: Identification with monoclonal antibodies and reproductive response to diet

Cited by 22 publications

References 16 publications

Effects of diet on development and reproduction of the predatory pentatomids Picromerus bidens and Podisus maculiventris

Effects of diet on development and reproduction of the predatory pentatomids Picromerus bidens and Podisus maculiventris

Diet affects reproduction and number of oocytes per ovary of the predator Podisus nigrispinus (Dallas) (Heteroptera: Pentatomidae)

Egg detection in females of the polyphagous predator Macrolophus pygmaeus (Heteroptera: Miridae) by serological techniques

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