Vitamins determination by TLC/HPTLC—a mini-review

Ullah, Qasim; Mohammad, Ali

doi:10.1007/s00764-020-00051-y

Cited by 12 publications

(4 citation statements)

References 21 publications

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“…2 It has also been associated with several pharmacological applications, such as relieving the common cold 3 and showing an inverse relationship with cardiovascular mortality. 4 Moreover, the application of AA has been studied during chemotherapy, which can escalate the survival rate and life span in humans who have ovarian cancer. 5 Lastly, AA is also imperative for neuronal repair and can be used to treat Alzheimer's disease.…”

Section: ■ Introductionmentioning

confidence: 99%

“…AA is involved in collagen, carnitine, and norepinephrine production in gene transcription and histone production . It has also been associated with several pharmacological applications, such as relieving the common cold and showing an inverse relationship with cardiovascular mortality . Moreover, the application of AA has been studied during chemotherapy, which can escalate the survival rate and life span in humans who have ovarian cancer .…”

Section: Introductionmentioning

confidence: 99%

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Environment-Friendly Approach to Isolate Ascorbic Acid from Plants and Micro-identified Using Fourier Transform Infrared Spectroscopy

Munir,

Jamal,

Inayatullah

2024

ACS Food Sci. Technol.

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Vitamin C [ascorbic acid (AA)] is an imperative antioxidant that humans require. Fruits and vegetables commonly contain AA. The application of titrimetric or chromatographic methods is generally performed to analyze AA; nevertheless, these approaches have several disadvantages such as being very costly for chromatographic methods and exhibiting food color interference upon using titration techniques. This study cruises the application of microwave treatment on AA compounds of plant samples and identified the AA level using Fourier transform infrared (FTIR) spectroscopy. The purposes of this study were to establish a validated method for the microwave-assisted isolation (MAI) of AA from plant samples. Validation studies such as linearity, selectivity, sensitivity, detection, and quantitation limits were evaluated using a standard AA solution. These methods were also applied for AA quantitation in oranges, guava, and spinach. The methods were selective and sensitive when applied to the standard solution. The FTIR spectroscopy approach was more sensitive, with lower values for detection limit (13.76 nmol•L −1 ) and quantitation limit (41.69 nmol•L −1 ), and more accurate with an error of less than 1%. The imperative AA groups are also identified, such as the hydroxyl groups found at 3297 nm −1 . In contrast, the aromatic group of AA can be obtained at 2084 cm −1 , although the absorption intensity is weak. The 1635 cm −1 atom is related to the carbonyl group (−C�O). The validated method was applied to investigate the AA level in plant samples, and all of them showed identical peaks with the standard, showing that the MAI has isolated the AA completely.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Environment-Friendly Approach to Isolate Ascorbic Acid from Plants and Micro-identified Using Fourier Transform Infrared Spectroscopy

Munir,

Jamal,

Inayatullah

2024

ACS Food Sci. Technol.

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“…It provides an easy, rapid and inexpensive method of screening without application of sophisticated equipment 4 . Both the TLC technique and high‐performance thin‐layer chromatography (HPTLC) technique are considered ideal candidates for hyphenation with bioautographic detection due to shortened time required for analysis 5,6 and large surface area for solvent evaporation, hence enhancing the tolerance of biological systems 7 . This is particularly important in the case of screening antimicrobial and enzymatic activities because organic solvents can cause death of cells or inactivation of enzymes 3 .…”

Section: Introductionmentioning

confidence: 99%

Development of a validated HPTLC‐bioautographic method for evaluation of aromatase inhibitory activity of plant extracts and their constituents

Dawood

Shawky

Hammoda

et al. 2021

Phytochemical Analysis

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Introduction Aromatase is a CYP450 enzyme that catalyses the conversion of androgens into oestrogens, where the decrease in the production of oestrogens aided by aromatase inhibitors is considered a target in post‐menopausal breast cancer therapy. TLC‐bioautography is a technique employed for combining chromatographic separations on TLC plates with bioassays. This is the first report to evaluate aromatase inhibitory activity using this technique. Objectives The aim of this study is to develop and validate a new TLC‐bioautographic method for determination of aromatase inhibitory activity in 14 plant extracts. Two quantitation methods, the peak area method and reciprocal iso‐inhibition volume (RIV) method, were compared and investigated to attain reliable results. Factors affecting the enzymatic reaction (temperature, pH, enzyme and substrate concentrations … etc.) were also investigated to attain the optimum parameters. Methodology TLC assisted by digital image processing was implemented for quantitative estimation of the aromatase inhibition of 14 plant extracts using chrysin as positive control. The fluorometric substrate dibenzyl fluorescein (DBF) was utilised for the assay, where inhibitory compounds were visualised as dark spots against a blue fluorescent background. Two software programs, Sorbfil® videodensitometer (in the peak area method) and ImageJ® (in the RIV method), were thoroughly validated using the International Council on Harmonisation (ICH) guideline and used for quantitation. Results The RIV method showed superiority over the peak area method in the quantitation results of the tracks with non‐homogenous background with %RSD values of 0.98 and 1.49 compared with 2.86 and 3.58, respectively. Further, the methods allow the comparison of the activity of different unknown inhibitory compounds without the need for a reference or a positive control. Conclusion Using the TLC‐bioautographic method by image processing combined with the RIV quantitation method, simultaneous separation and quantitation of aromatase inhibitory components could be applied to estimate the relative activity of various plant extracts.

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“…Описано применение тонкослойной хроматографии (ТСХ) и высокоэффективной тонкослойной хроматографии (ВЭТСХ) для разделения и идентификации витаминов, в том числе пиридоксина, из их смесей с использованием различных видов подвижных фаз, таких как изократические и смешанные растворители, а также подвижные растворители [7].…”

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Determination of Mexidol in tablets by the method of derivative spectrophotometry

Kvachakhiya,

Shormanov,

Maksina

2023

Humans and their Health

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Mexidol (3-hydroxy-6-methyl-2-ethylpyridine succinate) is a drug that exhibits neuroprotective, antihypoxic, anti-ischemic, nootropic, anti-stress and geroprotective effects and can suppress or slow down oxidation reactions. Оbjective: to develop a selective and simple method for assessing the content of mexidol in tablets. Materials and methods. The objects of the study were mexidol (3-hydroxy-6-methyl-2-ethylpyridine succinate) and Mexidol tablets 0.125 g. Derivative spectrophotometry was used as an analysis method. Results. The ability of mexidol to intensively absorb electromagnetic radiation in the quartz ultraviolet region was revealed. This phenomenon is due to the fact that the analyte molecule contains a number of chromophore groups: a system of conjugated aromatic bonds of the pyridine ring, unpaired electrons at the nitrogen atom, and an enol hydroxyl conjugated with the aromatic system In the region of 292 nm, there is a linear dependence of the derivatives of the 2nd order of the specific absorption coefficient of mexidol on the amount of analyte in the photometric solution (range 2.5-50.0 μg/ml). A calibration graph has been constructed, and its equation has been calculated by the least-squares method. The correlation coefficient exceeds 0.999. The limits of detection and quantification are 1.25 and 2.5 µg/ml, respectively. The technique is characterized by the necessary reproducibility and correctness. The values of the standard deviation and relative standard deviation in the determination of the analyte using the proposed method (n=6; P=0.95) in the tablet mass are 1.16 and 0.012, respectively, in tablets of 0.125 g, 1.32 and 0.013, respectively. Conclusion. A simple and selective method has been developed for estimating the quantitative content of mexidol in 0.125 g tablets based on the calculation of second-order derivatives of spectra.

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Vitamins determination by TLC/HPTLC—a mini-review

Cited by 12 publications

References 21 publications

Environment-Friendly Approach to Isolate Ascorbic Acid from Plants and Micro-identified Using Fourier Transform Infrared Spectroscopy

Environment-Friendly Approach to Isolate Ascorbic Acid from Plants and Micro-identified Using Fourier Transform Infrared Spectroscopy

Development of a validated HPTLC‐bioautographic method for evaluation of aromatase inhibitory activity of plant extracts and their constituents

Determination of Mexidol in tablets by the method of derivative spectrophotometry

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