Vitamin K‐dependent coagulation factors and fibrinogen levels in FFP remain stable upon repeated freezing and thawing

Ben‐Tal, Ofira; Zwang, Eti; Eichel, Roza; Badalbev, Tanya; Hareuveni, Mara

doi:10.1046/j.1537-2995.2003.00444.x

Cited by 29 publications

(45 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean ± SE activities of coagulation factors and fibrinogen in various blood groups at day 1 and day 5 were as follows: Factor II-86.3 ± 1.7, Factor V-95.4 ± 3.2, Factor VII-93.6 ± 4.9, Factor VIII-89.5 ± 4.9, Factor X-100.3 ± 3.6, Factor XI-105.0 ± 4.8, fibrinogen-322.3 ± 9.1 and Factor II-88.5 ± 2.2, Factor V-94.4 ± 3.0, Factor VII-90.2 ± 7.9, Factor VIII-76.7 ± 4.8, Factor X-101.5 ± 3.3, Factor XI-106.5 ± 5.1, fibrinogen-316.2 ± 9.8, respectively. Since Ben-Tal and co-workers reported no difference between blood groups for factors II, IX, X, and fibrinogen except for factor VII, factor data analysis from 20 FFP units was combined as one group for each factor [9]. All aerobic and anaerobic cultures of samples taken on day 5 of storage were negative at 7 days.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Study of coagulation factor activities in apheresed thawed fresh frozen plasma at 1–6°C for five days

Sidhu

Brimhall

et al. 2006

J. Clin. Apheresis

View full text Add to dashboard Cite

The concern for the loss of activities of coagulation factors in thawed fresh frozen plasma kept at 1-6 degrees C for long periods has prevented transfusion services from using thawed plasma beyond 24 hours of storage. There is no mention of the method of collection of the plasma and/or the study of the bacterial growth in the studies reported in the literature. The present project was undertaken to investigate coagulation factor activities and bacterial growth in apheresed fresh plasma. Twenty apheresed plasma units from different blood groups were used. After the 24-hour expiration time of the thawed plasma kept at 1-6 degrees C, aliquots were taken at day 1, day 3, and day 5 of expiration time and were immediately frozen at -70 degrees C. Samples were assayed for activities of coagulation factors II, V, VII, VIII, X, XI, and fibrinogen (Fib). Our study reveals no statistically significant change in activities of coagulation factors II, VII, X, XI, and fibrinogen from day 1 to day 5 storage of plasma at 1-6 degrees C; however, there is a mean decrease of 8.8 and 14.3% in activities of factors V and VIII, respectively. All culture samples taken on day 5 storage were negative at 7 days. In conclusion, our results do not show a significant change in the activity of most coagulation factors in the thawed apheresis plasma stored at 1-6 degrees C over a 5-day period. Hence, it is feasible to transfuse the plasma beyond the 24-hour period without compromising the clinical outcome of patients with coagulopathy.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Various investigators studied the stability of the coagulation factors either at room temperature or at 1-6°C for 24 hours or thawing with subsequent freezing after storage for 24 hours at 1-6°C [1][2][3]. Three recent reports have studied the stability of coagulation factors over 5 to 10 days [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Study of coagulation factor activities in apheresed thawed fresh frozen plasma at 1–6°C for five days

Sidhu

Brimhall

et al. 2006

J. Clin. Apheresis

View full text Add to dashboard Cite

show abstract

“…This point has received attention for its possible effects on the reliability of analytical procedures on clinical samples [66] and on the therapeutic efficacy of fresh-frozen plasma (FFP) preparations [67]. Quality of FFP appears to depend both on freezing [68] and on thawing [69,70] conditions.…”

Section: Discussionmentioning

confidence: 99%

Any use in proteomics for low-tech approaches? Detecting fibrinogen chains of different animal species in two-dimensional electrophoresis patterns

Miller

Gianazza

Gemeiner

2010

Journal of Chromatography B

View full text Add to dashboard Cite

“…FFP is used as a hemostatic support either for treatment or for prophylaxis. [2] FFP should not be used as a volume expander or as a nutritional source. [3]…”

Section: Introductionmentioning

confidence: 99%

The effect of repeated freezing and thawing on levels of vitamin K-dependent coagulation factors and fibrinogen in fresh frozen plasma

Philip

Sarkar

Pathak

2013

Asian J Transfus Sci

View full text Add to dashboard Cite

Background:Fresh frozen plasma (FFP) is considered adequate for transfusion immediately after thawing or for up to 24 hours if kept at 1–6°C, and is currently used very often to replace deficient clotting factors. If factor levels in refrozen FFP are within normal limits, then this component can possibly be transfused, thus avoiding wastage of FFP.Aim:To study the fate of vitamin K-dependent coagulation factors (F II, F VII, F IX, F X) and fibrinogen activity levels in repeatedly (twice) frozen and thawed FFP.Materials and Methods:Two hundred FFP units comprising 50 units of each major blood group (A, B, AB, and O) were thawed at 37°C and 10–20 mL of FFP transferred to transfer bags with the help of a sterile connecting device (SCD). The FFP samples were taken into tubes (first sampling), and then the transfer bags were kept for 24 hours at 4°C. After 24 hours, repeat samples were taken in tubes from the transfer bag (second sampling), and then the bags were re-stored at < -18°C. One week later, the above procedure was repeated. Activity of coagulation factors and fibrinogen levels were measured by the automated coagulation analyzer.Results:The levels of F II, F VII, F IX, F X, and fibrinogen of all the 200 FFP units, at all four time points, were above the lower normal value, but well within the normal range.Conclusion:The levels of F II, F VII, F IX, F X, and fibrinogen remain stable and adequate for transfusion in twice-thawed-and-refrozen FFP. This component can be safely used for transfusion as a source of vitamin K-dependent clotting factors and fibrinogen.

show abstract

Vitamin K‐dependent coagulation factors and fibrinogen levels in FFP remain stable upon repeated freezing and thawing

Abstract: The levels of prothrombin, FVII, F IX, FX, and fibrinogen remain stable and adequate for transfusion in twice-thawed-and-refrozen FFP. This component can be safely used for transfusion as a source of vitamin K-dependent clotting factors and fibrinogen.

Cited by 29 publications

References 23 publications

Study of coagulation factor activities in apheresed thawed fresh frozen plasma at 1–6°C for five days

Study of coagulation factor activities in apheresed thawed fresh frozen plasma at 1–6°C for five days

Any use in proteomics for low-tech approaches? Detecting fibrinogen chains of different animal species in two-dimensional electrophoresis patterns

The effect of repeated freezing and thawing on levels of vitamin K-dependent coagulation factors and fibrinogen in fresh frozen plasma

Contact Info

Product

Resources

About