Vitamin E treatment decreases muscle injury in mdx mice

Mâncio, Rafael Dias; Hermes, Túlio de Almeida; Macedo, Aline Barbosa; Mizobuti, Daniela Sayuri; Valduga, Amanda Harduim; Rupcic, Ian Feller; Minatel, Elaine

doi:10.1016/j.nut.2017.07.003

Cited by 21 publications

(16 citation statements)

References 37 publications

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“…3 The diaphragm muscle (DIA) from mdx mice is what most resembles the dystrophic human pathology, 22 expressing increased oxidative stress markers (such as DHE, lipofuscin granules and the 4-HNE protein adduct) and reduction in SOD and catalase levels. 23,24 Differing from the diaphragm muscle, limb muscles from mdx mice have high levels and activity of antioxidant enzymes, such as SOD, catalase and GPx. 25 The mild limb muscle phenotype shown in mdx mice has been attributed to this variance.…”

Section: Discussionmentioning

confidence: 99%

Tempol improves redox status in mdx dystrophic diaphragm muscle

Hermes

Mizobuti

Rocha

et al. 2020

Int J Experimental Path

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Several studies have demonstrated that oxidative stress plays a critical function in the pathogenesis of Duchenne muscular dystrophy (DMD). 1-3 DMD is the most frequent and lethal muscular dystrophy, affecting 1 in 3000-6000 male children, 3 and at present, there is no efficient therapy for this dystrophy. 4 Mitochondria are one of the main sources of reactive oxygen species (ROS). Hydroxyl radical, superoxide anion, hydrogen peroxide and singlet oxygen constitute the ROS, and when produced in excess or not properly controlled, it becomes toxic. 5,6 There is feed-forward looping; the high levels of ROS produced by the mitochondrial electron transport chain damage the mitochondria and consequently lead to even greater production of ROS and mitochondrial injury. 6

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Section: Discussionmentioning

confidence: 99%

Tempol improves redox status in mdx dystrophic diaphragm muscle

Hermes

Mizobuti

Rocha

et al. 2020

Int J Experimental Path

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“…Thus, NAC-treatment had more profound effects than vitamin E. Vitamin E supplementation has also been evaluated in dystrophin-deficient mice. A two-week supplementation of vitamin E in mdx mice diminished inflammation and oxidative stress in diaphragm muscle [31]. In 1960 there was a clinical trial of high dosage of vitamin E in human muscular dystrophy patients, but it was concluded that vitamin E did not produce a positive response compared with placebo [54].…”

Section: Discussionmentioning

confidence: 99%

“…The administrated dose and treatment duration for NAC was based on a number of studies where 150 mg/kg NAC has been used [28][29][30]. For vitamin E administration, we adapted the protocol of Mancio et al, demonstrating that 40 mg vitamin E/kg daily oral gavage for two weeks reduced muscular dystrophy in mdx mice [31].…”

Section: Muscle Force Loss Is Prevented By Nac Treatmentmentioning

confidence: 99%

Antioxidants Reduce Muscular Dystrophy in the dy2J/dy2J Mouse Model of Laminin α2 Chain-Deficient Muscular Dystrophy

et al. 2020

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Congenital muscular dystrophy with laminin α2 chain-deficiency (LAMA2-CMD) is a severe neuromuscular disorder without a cure. Using transcriptome and proteome profiling as well as functional assays, we previously demonstrated significant metabolic impairment in skeletal muscle from LAMA2-CMD patients and mouse models. Reactive oxygen species (ROS) increase when oxygen homeostasis is not maintained and, here, we investigate whether oxidative stress indeed is involved in the pathogenesis of LAMA2-CMD. We also analyze the effects of two antioxidant molecules, N-acetyl-L-cysteine (NAC) and vitamin E, on disease progression in the dy 2J /dy 2J mouse model of LAMA2-CMD. We demonstrate increased ROS levels in LAMA2-CMD mouse and patient skeletal muscle. Furthermore, NAC treatment (150 mg/kg IP for 6 days/week for 3 weeks) led to muscle force loss prevention, reduced central nucleation and decreased the occurrence of apoptosis, inflammation, fibrosis and oxidative stress in LAMA2-CMD muscle. In addition, vitamin E (40 mg/kg oral gavage for 6 days/week for 2 weeks) improved morphological features and reduced inflammation and ROS levels in dy 2J /dy 2J skeletal muscle. We suggest that NAC and to some extent vitamin E might be potential future supportive treatments for LAMA2-CMD as they improve numerous pathological hallmarks of LAMA2-CMD.

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“…It also has an effect on the expression of selected genes (e.g., connective tissue growth factor (CTGF) and others), which are important in wound repair and regeneration [ 38 , 39 , 60 ]. In a very recent study by Mancio et al, vitamin E improved skeletal muscle injury and promoted membrane repair in mouse models [ 61 ]. There are numerous studies which suggest that tocopherols can help in preventing or modulating diseases associated with oxidative stress, such as cardiovascular diseases [ 62 ] and cancers [ 62 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

Ciebiera

Szymańska-Majchrzak

Sentkowska

et al. 2018

BioMed Research International

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Uterine fibroids (UFs) are benign tumors of the reproductive tract, arising from smooth muscle cells of the uterus. Steroid hormones, estrogen, and progesterone are considered to be the most important links in the pathophysiology of UFs. Alpha-tocopherol (AT) is the most active form of vitamin E. What is important as far as UFs are concerned is that ATs contain structural determinants, which makes them possible ligands for estrogen receptors (ERs). We present a retrospective cohort study performed in a university teaching hospital. We included a total of 162 patients divided into 2 groups: with UFs and controls. The effects of age, body mass index (BMI), positive medical history, parity, and AT serum concentrations on the risk for the development of UFs were investigated. Mean AT serum concentrations were 11.66 ± 4.97 μg/ml and 7.83 ± 3.13 μg/ml (medians 10.56 μg/ml and 7.42 μg/ml) in patients with UFs confirmed on ultrasound and controls, respectively. The presented difference was statistically significant. Higher BMI, positive family history, and low parity were found to be major risk factors for UFs. In our study, we confirmed that elevated serum AT concentration might be an important risk factor for UFs in Caucasian women. Further research in this area is necessary.

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Vitamin E treatment decreases muscle injury in mdx mice

Cited by 21 publications

References 37 publications

Tempol improves redox status in mdx dystrophic diaphragm muscle

Tempol improves redox status in mdx dystrophic diaphragm muscle

Antioxidants Reduce Muscular Dystrophy in the dy2J/dy2J Mouse Model of Laminin α2 Chain-Deficient Muscular Dystrophy

Alpha-Tocopherol Serum Levels Are Increased in Caucasian Women with Uterine Fibroids: A Pilot Study

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