Vitamin E Protects against Photoinhibition and Photooxidative Stress in <i>Arabidopsis thaliana</i>

Havaux, Michel; Eymery, Françoise; Porfirova, Svetlana; Rey, Pascal; Dörmann, Peter

doi:10.1105/tpc.105.037036

Cited by 456 publications

(414 citation statements)

References 79 publications

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“…Furthermore, plastoquinone and phylloquinone are critical for photosynthetic function as electron acceptors and donors, whereas tocopherol is an important protector against oxidative damage to the thylakoid membrane (Havaux et al, 2005;Kanwischer et al, 2005). The presence of proteins somehow involved with lipid metabolism and/or breakdown is also consistent with the proposition that PGs form a lipid/fatty acid storage space (Fig.…”

Section: Changes In Pg Proteome Composition After Prolonged Hl Stresssupporting

confidence: 70%

Protein Profiling of Plastoglobules in Chloroplasts and Chromoplasts. A Surprising Site for Differential Accumulation of Metabolic Enzymes

2006

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Plastoglobules (PGs) are oval or tubular lipid-rich structures present in all plastid types, but their specific functions are unclear. PGs contain quinones, a-tocopherol, and lipids and, in chromoplasts, carotenoids as well. It is not known whether PGs contain any enzymes or regulatory proteins. Here, we determined the proteome of PGs from chloroplasts of stressed and unstressed leaves of Arabidopsis (Arabidopsis thaliana) as well as from pepper (Capsicum annuum) fruit chromoplasts using mass spectrometry. Together, this showed that the proteome of chloroplast PGs consists of seven fibrillins, providing a protein coat and preventing coalescence of the PGs, and an additional 25 proteins likely involved in metabolism of isoprenoid-derived molecules (quinines and tocochromanols), lipids, and carotenoid cleavage. Four unknown ABC1 kinases were identified, possibly involved in regulation of quinone monooxygenases. Most proteins have not been observed earlier but have predicted N-terminal chloroplast transit peptides and lack transmembrane domains, consistent with localization in the PG lipid monolayer particles. Quantitative differences in PG composition in response to high light stress and degreening were determined by differential stable-isotope labeling using formaldehyde. More than 20 proteins were identified in the PG proteome of pepper chromoplasts, including four enzymes of carotenoid biosynthesis and several homologs of proteins observed in the chloroplast PGs. Our data strongly suggest that PGs in chloroplasts form a functional metabolic link between the inner envelope and thylakoid membranes and play a role in breakdown of carotenoids and oxidative stress defense, whereas PGs in chromoplasts are also an active site for carotenoid conversions.

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Section: Changes In Pg Proteome Composition After Prolonged Hl Stresssupporting

confidence: 70%

Protein Profiling of Plastoglobules in Chloroplasts and Chromoplasts. A Surprising Site for Differential Accumulation of Metabolic Enzymes

2006

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“…Tocopherol accumulates in plastoglobules under high light stress (Havaux et al 2005;Brehelin et al 2007) and may in part explain the enlargement of PG under such conditions. VTE1 mediates the conversion of 2,3-dimethyl-5-phytyl-1,4-benzoquinol (DMPBQ) into c-tocopherol.…”

Section: Implication Of Plastoglobules In Isoprenoid Synthesismentioning

confidence: 99%

A mechanism implicating plastoglobules in thylakoid disassembly during senescence and nitrogen starvation

Besagni

Kessler

2012

Planta

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Plastoglobules are lipid droplets present in all plastid types. In chloroplasts, they are connected to the thylakoid membrane by the outer lipid half-bilayer. The plastoglobule core is composed of neutral lipids most prominently the prenylquinones, triacylglycerols, fatty acid phytyl esters but likely also unknown compounds. During stress and various developmental stages such as senescence, plastoglobule size and number increase due to the accumulation of lipids. However, their role is not limited to lipid storage. Indeed, the characterization of the plastoglobule proteome revealed the presence of enzymes. Importantly it has been demonstrated that these participate in isoprenoid lipid metabolic pathways at the plastoglobule, notably in the metabolism of prenylquinones. Recently, the characterization of two phytyl ester synthases has established a firm metabolic link between PG enzymatic activity and thylakoid disassembly during chloroplast senescence and nitrogen starvation.

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“…lines were issued from the Columbia ecotype (Col-0). The vte1 npq1, ch1, and Cat(2) plants have been previously described (Vandenabeele et al, 2004;Havaux et al, 2005Havaux et al, , 2007. They were cultivated on soil under low light (100 mmol m 22 s 21 ; day/night 8/16; 20°C/18°C; 70% relative humidity) and normal (380 ppm) or high CO 2 (2,000 ppm) for 4 to 5 weeks.…”

Section: Plant Materials Growth and Stress Conditionsmentioning

confidence: 99%

“…Spontaneous photon emission from whole Arabidopsis plants was measured after 2 h dark adaptation with a liquid N 2 cooled CCD camera, as previously described (Havaux et al, 2005(Havaux et al, , 2007. Integration time was 20 min.…”

Section: Lpo Analysesmentioning

confidence: 99%

“…Next, we compared HOTE isomer distribution in leaves from Arabidopsis wild-type and vte1 npq1 plants. The vte1 npq1 double mutant is sensitive to photooxidative stress due to a deficiency in two lipophilic 1 O 2 quenchers in the chloroplasts, a-tocopherol and the carotenoid zeaxanthin (Havaux et al, 2005). In both wild-type and vte1 npq1 leaves, basal levels of HOTE (approximately 5-10 nmol/g FW; Fig.…”

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confidence: 99%

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Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants

Triantaphylidès¹,

Krischke²,

Hoeberichts³

et al. 2008

Plant Physiology

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498

358

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Reactive oxygen species act as signaling molecules but can also directly provoke cellular damage by rapidly oxidizing cellular components, including lipids. We developed a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry-based quantitative method that allowed us to discriminate between free radical (type I)-and singlet oxygen ( 1 O 2 ; type II)-mediated lipid peroxidation (LPO) signatures by using hydroxy fatty acids as specific reporters. Using this method, we observed that in nonphotosynthesizing Arabidopsis (Arabidopsis thaliana) tissues, nonenzymatic LPO was almost exclusively catalyzed by free radicals both under normal and oxidative stress conditions. However, in leaf tissues under optimal growth conditions, 1 O 2 was responsible for more than 80% of the nonenzymatic LPO. In Arabidopsis mutants favoring 1 O 2 production, photooxidative stress led to a dramatic increase of 1 O 2 (type II) LPO that preceded cell death. Furthermore, under all conditions and in mutants that favor the production of superoxide and hydrogen peroxide (two sources for type I LPO reactions), plant cell death was nevertheless always preceded by an increase in 1 O 2 -dependent (type II) LPO. Thus, besides triggering a genetic cell death program, as demonstrated previously with the Arabidopsis fluorescent mutant, 1 O 2 plays a major destructive role during the execution of reactive oxygen species-induced cell death in leaf tissues.Plant leaves capture sun-derived light energy to drive CO 2 fixation during photosynthesis. During this process, leaves need to cope with photooxidative stress when the balance between energy absorption and consumption is disturbed. Excess excitation energy in the photosystems (PSI and PSII) leads to the inhibition of photosynthesis via the production of various reactive oxygen species (ROS) at different spatial levels of the cell (Apel and Hirt, 2004;Asada, 2006;Van Breusegem and Dat, 2006). Both exposure to high light intensities and decreased CO 2 availability direct linear electron transfer toward the reduction of molecular oxygen, generating superoxide radicals (O 2 2. ) at PSI (the Mehler reaction). Superoxide dismutation generates hydrogen peroxide (H 2 O 2 ), which is detoxified in the chloroplast by ascorbate peroxidases. As such, this so-called water-water cycle participates in the dissipation of excess energy (Asada, 2006). Decreased CO 2 availability affects the first step in CO 2 fixation by shifting the carboxylation of Rubisco by the Rubisco carboxylase-oxygenase enzyme toward oxygenation, a process called photorespiration. This leads, through the action of glycolate oxidase, to peroxisomal H 2 O 2 production that is counteracted by catalases. Finally, when the intersystem electron carriers are overreduced, triplet excited P680 in the PSII reaction center as well as triplet chlorophylls in the light-harvesting antennae are produced, with the production of singlet oxygen ( 1 O 2 ) as a consequence (Krieger-Liszkay, 2005). In photosynthetic membranes, 1 O 2 ...

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Vitamin E Protects against Photoinhibition and Photooxidative Stress in Arabidopsis thaliana

Cited by 456 publications

References 79 publications

Protein Profiling of Plastoglobules in Chloroplasts and Chromoplasts. A Surprising Site for Differential Accumulation of Metabolic Enzymes

Protein Profiling of Plastoglobules in Chloroplasts and Chromoplasts. A Surprising Site for Differential Accumulation of Metabolic Enzymes

A mechanism implicating plastoglobules in thylakoid disassembly during senescence and nitrogen starvation

Singlet Oxygen Is the Major Reactive Oxygen Species Involved in Photooxidative Damage to Plants

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