Vitamin E down-modulates mitogen-activated protein kinases, nuclear factor-κB and inflammatory responses in lung epithelial cells

Ekstrand‐Hammarström, Barbro; Österlund, Camilla; Lilliehöök, Bo; Bucht, Anders

doi:10.1111/j.1365-2249.2006.03285.x

Cited by 54 publications

(37 citation statements)

References 53 publications

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“…The levels of MCP-1 did not increase over the background level when stimulated with any of the allergens. Consistent with previous studies by us, background expression by unstimulated NHBE cells was generally higher than baseline expression by BEAS-2B cells [14,22]. …”

Section: Resultssupporting

confidence: 92%

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Non-Proteolytic Aeroallergens from Mites, Cat and Dog Exert Adjuvant-Like Activation of Bronchial Epithelial Cells

Österlund

Grönlund

Gafvelin

et al. 2010

Int Arch Allergy Immunol

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Background: Exposure to seasonal or indoor allergens may cause sensitisation and development of allergic airway diseases. We have previously demonstrated that the non-proteolytic major house dust mite (HDM) allergen Der p 2 stimulates pro-inflammatory responses in bronchial epithelial cells. We aimed to determine if other clinically relevant non-proteolytic aeroallergens originating from HDMs, storage mites, cat, dog, birch and timothy also activate respiratory epithelial cells. Methods: Cultures of human bronchial epithelial cell line BEAS-2B, normal human bronchial epithelial cells and alveolar epithelial cell line A549 were exposed to recombinant (r)Der p 2, natural (n)Der f 2, rEur m 2, rLep d 2, rFel d 1, nFel d 1, rCan f 2, rBet v 1 or rPhl p 5a. A panel of secreted mediators and expression of cell adhesion receptors involved in recruitment, survival and adhesion of inflammatory cells in asthmatic airways was assessed. Results: The mite allergens rDer p 2, nDer f 2, rEur m 2 and rLep d 2 as well as the cat and dog allergens rFel d 1, nFel d 1 and rCan f 2 induced granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, interleukin (IL)-6, IL-8, monocyte-chemotactic protein-1 and macrophage inflammatory protein-3α secretion from bronchial epithelial cells as well as surface expression of intracellular adhesion molecule-1. The pollen allergens rBet v 1 and rPhl p 5a from birch and timothy did not activate the cells. None of the studied allergens affected the alveolar epithelial cells. Conclusion: These results show that both mite and structurally unrelated cat and dog allergens can activate respiratory epithelial cells by adjuvant-like protease-independent mechanisms.

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Section: Resultssupporting

confidence: 92%

“…The observed differences in magnitude between the responses mounted by BEAS-2B as compared with NHBE cells might thus be explained by the immortalised phenotype of BEAS-2B. This is also consistent with results from previous studies by us [14,22]. We observed some differences in responsiveness to recombinant and natural Fel d 1.…”

Section: Discussionsupporting

confidence: 92%

Non-Proteolytic Aeroallergens from Mites, Cat and Dog Exert Adjuvant-Like Activation of Bronchial Epithelial Cells

Österlund

Grönlund

Gafvelin

et al. 2010

Int Arch Allergy Immunol

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“…α-tocopherol also inhibits respiratory burst in human macrophages via a mechanism involving PKC inhibition, followed by attenuation of p47 (phox) phosphorylation and membrane translocation [29] . Increased NF-κB activity is found in inflamed intestinal mucosa; and factors implicated in IBD, such as TNF-α, LPS, and IL-1, are potent activators of NF-κB [30] . On the other hand, reactive oxygen species, the hypothetical mediators of IBD flare-ups, have been implicated in the stimulation of the signal transduction pathway involving NF-κB.…”

Section: Discussionmentioning

confidence: 99%

Rectal administration of d-alpha tocopherol for active ulcerative colitis: A preliminary report

Mirbagheri¹,

Nezami²,

Assa³

et al. 2008

WJG

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AIM:To investigate the anti-oxidant and antineutrophil recruitment effects of rectal d-alpha (d-α) tocopherol administration on mild and moderately active ulcerative colitis (UC). METHODS: Fifteen patients with mild and moderately active ulcerative colitis were enrolled in an openlabel study of d-α tocopherol enema (8000 U/d) for 12 wk. All patients were receiving concomitant therapy with 5-aminosalicylic acid derivatives (5-ASA) and/or immunomodulator medications. Endoscopic evaluation was performed at baseline and after 4th and 12th weeks. Disease activity was measured with the Mayo disease activity index (DAI) and remission was defined as DAI of ≤ 2 with no blood in stool. Clinical response was defined as a DAI reduction of ≥ 2. RESULTS: At the end of 12th week, the average DAI score significantly decreased compared to the beginning of the study (2.3 ± 0.37 vs 8 ± 0.48, P < 0.0001). One patient was withdrawn after 3 wk for being unavailable to follow-up. On the 4th week of therapy, 12 patients showed clinical response, 3 of whom (21.4%) achieving remission. After 12 wk, all 14 patients responded clinically to the therapy and remission was induced in 9 of them (64%). No patient reported adverse events or was hospitalized due to worsened disease activity. CONCLUSION: This preliminary report suggests that rectal d-α tocopherol may represent a novel therapy for mild and moderately active UC. The observed results might be due to the anti-inflammatory and antioxidative properties of vitamin E.

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“…According to EkstrandHammarström et al (8), after application of vitamin E, inflammation can be inhibited as a result of the inhibition of proinflammatory cytokine production, reduced production of acute phase proteins, and a reduced inflammatory response in the lung epithelial cells.…”

Section: Resultsmentioning

confidence: 99%

Efficacy of florfenicol and flunixin followed with vitamins E and/or C on selected oxidative and inflammatory mechanisms in young cattle under transport and adaptation stress

Wernicki¹,

STACHURA²,

HOLA³

et al. 2018

Medycyna Weterynaryjna

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SummaryBovine respiratory disease (BRD) in cattle, due to the participation of numerous viral and bacterial etiological agents, predisposition factors, and environmental stresses, is a serious health and economic problem in herds of beef cattle. Given the broad scope of the problem of respiratory syndrome and the limited immunoprophylaxis, the aim of this study was to estimate the additive effects of flunixin with florfenicol followed with vitamin E or/and C in reducing the development of oxidative stress and inflammation in the first weeks in the feedlot.The study was conducted on young Simmental cattle (n = 50) weighing approx. 160 kg. The animals were divided into 5 groups (n = 10 in each group). Group I was the control. The cattle received florfenicol and flunixin in combination with vitamins E or C (Group II and III), florfenicol and flunixin followed with vitamin E and C (Group IV), or florfenicol and flunixin without vitamins (group V). Blood for sera was collected on the day the drugs were administered and on days 3, 7, 14, 21 and 28 of the experiment. The level of oxidative stress was analysed on the basis of the concentration of nitrogen ions (NO) in the sera and of lipid peroxidation end products reacting with thiobarbituric acid (TBARS). Concentrations of haptoglobin (Hp) and serum amyloid A (SAA) in the sera were determined using EIA enzyme immunoassays.The treatment used in the study, involving the application of florfenicol and flunixin with vitamin E or C, substantially reduced the level of oxidative stress, expressed as a reduced concentration of TBARS and NO ions, particularly in groups II and III. It was also confirmed that the treatment inhibited the concentration of acute phase proteins (Hp and SAA).

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Vitamin E down-modulates mitogen-activated protein kinases, nuclear factor-κB and inflammatory responses in lung epithelial cells

Cited by 54 publications

References 53 publications

Non-Proteolytic Aeroallergens from Mites, Cat and Dog Exert Adjuvant-Like Activation of Bronchial Epithelial Cells

Non-Proteolytic Aeroallergens from Mites, Cat and Dog Exert Adjuvant-Like Activation of Bronchial Epithelial Cells

Rectal administration of d-alpha tocopherol for active ulcerative colitis: A preliminary report

Efficacy of florfenicol and flunixin followed with vitamins E and/or C on selected oxidative and inflammatory mechanisms in young cattle under transport and adaptation stress

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