Vitamin E and selenium partially prevent cytotoxicity, oxidative stress and DNA damage induced by T-2 toxin in bovine Leydig cells

Yang, Jian Ying; Du, Jie; Zhang, Yong Fa; Li, Yuan Xiao

doi:10.1016/j.theriogenology.2022.06.028

Cited by 7 publications

(6 citation statements)

References 44 publications

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“…Interestingly, the digestive enzyme applied in different animals was distinct. Collagenase II was used in mice and rats, whereas trypsin and collagenase II mixture was applied to cows ( 38 ) and pigs ( 39 ). With regard to poultry (such as chicken) ( 40 ), successive digestion of trypsin and collagenase II was carried out in the acquisition of cell suspension.…”

Section: Discussionmentioning

confidence: 99%

Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos)

Chu

Javed

Ashraf³

et al. 2023

Front. Endocrinol.

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Testicular Leydig cells (LCs) are the primary known source of testosterone, which is necessary for maintaining spermatogenesis and male fertility. However, the isolation, identification, and functional analysis of testosterone in duck LCs are still ambiguous. The aim of the present study was to establish a feasible method for isolating highly purified primary duck LCs. The highly purified primary duck LCs were isolated from the fresh testes of 2-month-old ducks via the digestion of collagenase IV and Percoll density gradient centrifugation; hematoxylin and eosin (H&E), immunohistochemistry (IHC) staining, ELISA, and radioimmunoassay were performed. Results revealed that the LCs were prominently noticeable in the testicular interstitium of 2-month-old ducks as compared to 6-month-old and 1-year-old ducks. Furthermore, IHC demonstrated that the cultured LCs occupied 90% area of the petri dish and highly expressed 3β-HSD 24 h after culture (hac) as compared to 48 and 72 hac. Additionally, ELISA and radioimmunoassay indicate that the testosterone level in cellular supernatant was highly expressed in 24 and 48 hac, whereas the testosterone level gradually decreased in 72 and 96 hac, indicating the primary duck LCs secrete testosterone at an early stage. Based on the above results, the present study has effectively developed a technique for isolating highly purified primary duck LCs and identified its biological function in synthesizing testosterone.

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Section: Discussionmentioning

confidence: 99%

Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos)

Chu

Javed

Ashraf³

et al. 2023

Front. Endocrinol.

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“…NAC (5 mmol/L) attenuated T-2 (8.10 nmol/L)-induced oxidative stress, apoptosis, and functional impairment in TM4 cells (testes-supporting cells) ( Yang et al., 2021 ). In isolated bovine testicular mesenchymal cells, vitamin E and selenium significantly upregulated GSH-Px, SOD, and CAT activities, thereby reducing oxidative damage to ameliorate T-2-induced germ cell toxicity ( Yang et al., 2022 ).…”

Section: Effective Protective Agents Against Organ Toxicity Of T-2 An...mentioning

confidence: 99%

“… Preventing oxidative stress and DNA damage. Yang et al. (2022) Se Rats Pre-supplementation Se (0.5 and 2.5 mg/kg feed for 6 weeks); T-2 (3.8 mg/kg BW).…”

Section: Introductionmentioning

confidence: 99%

Effective protective agents against the organ toxicity of T-2 toxin and corresponding detoxification mechanisms: A narrative review

Wang,

Sun,

Wang

et al. 2024

Animal Nutrition

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“…Fu et al found that ZEA can cooperate with LPS to increase the accumulation of ROS and malondialdehyde (MDA) in bovine mammary epithelial cells (MAC-T), and reduce their mitochondrial membrane potential, superoxide dismutase (SOD), and glutathione (GSH) levels, to aggravate cytotoxicity [ 74 ]. T-2 toxin exposure could cause oxidative stress–induced cytotoxicity by significantly reducing cell viability, increasing MDA levels, and reducing glutathione peroxidase (GSH-Px), SOD, and catalase (CAT) activity [ 75 ]. All these findings suggest that cellular oxidative stress is one of the modes of virulence induced by mycotoxins.…”

Section: Major Toxicity Mechanisms Induced By Mycotoxins and Heavy Me...mentioning

confidence: 99%

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

Sun

Chen

Xiong

et al. 2023

Biol Trace Elem Res

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Mycotoxins are secondary metabolites produced by specific fungi. More than 400 different mycotoxins are known in the world, and the concentration of these toxins in food and feed often exceeds the acceptable limit, thus causing serious harm to animals and human body. At the same time, modern industrial agriculture will also bring a lot of environmental pollution in the development process, including the increase of heavy metal content, and often the clinical symptoms of low/medium level chronic heavy metal poisoning are not obvious, thus delaying the best treatment opportunity. However, the traditional ways of detoxification cannot completely eliminate the adverse effects of these toxins on the body, and sometimes bring some side effects, so it is essential to find a new type of safe antidote. Trace element selenium is among the essential mineral nutrient elements of human and animal bodies, which can effectively remove excessive free radicals and reactive oxygen species in the body, and has the effects of antioxidant, resisting stress, and improving body immunity. Selenium is common in nature in inorganic selenium and organic selenium. In previous studies, it was found that the use of inorganic selenium (sodium selenite) can play a certain protective role against mycotoxins and heavy metal poisoning. However, while it plays the role of antioxidant, it will also have adverse effects on the body. Therefore, it was found in the latest study that selenium yeast could not only replace the protective effect of sodium selenite on mycotoxins and heavy metal poisoning, but also improve the immunity of the body. Selenium yeast is an organic selenium source with high activity and low toxicity, which is produced by selenium relying on the cell protein structure of growing yeast. It not only has high absorption rate, but also can be stored in the body after meeting the physiological needs of the body for selenium, so as to avoid selenium deficiency again in the short term. However, few of these studies can clearly reveal the protective mechanism of yeast selenium. In this paper, the detoxification mechanism of selenium yeast on mycotoxins and heavy metal poisoning was reviewed, which provided some theoretical support for further understanding of the biological function of selenium yeast and its replacement for inorganic selenium. The conclusions suggest that selenium yeast can effectively alleviate the oxidative damage by regulating different signaling pathways, improving the activity of antioxidant enzymes, reversing the content of inflammatory factors, regulating the protein expression of apoptosis-related genes, and reducing the accumulation of mycotoxins and heavy metals in the body.

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Vitamin E and selenium partially prevent cytotoxicity, oxidative stress and DNA damage induced by T-2 toxin in bovine Leydig cells

Cited by 7 publications

References 44 publications

Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos)

Primary culture and endocrine functional analysis of Leydig cells in ducks (Anas platyrhynchos)

Effective protective agents against the organ toxicity of T-2 toxin and corresponding detoxification mechanisms: A narrative review

Detoxification of Selenium Yeast on Mycotoxins and Heavy Metals: a Review

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