Vitamin E analysis by ultra-performance convergence chromatography and structural elucidation of novel α-tocodienol by high-resolution mass spectrometry

Natural 6-hydroxy-chromanols and -chromenols: structural diversity, biosynthetic pathways and health implications

“…a-Tocodienol (26) has recently been discovered as a trace compound (0.2% of the total vitamin E content) in palm oil. 30 Interestingly to note is a recent publication by Hammann et al, who have tentatively identied 170 unsaturated tocochromanol compounds in palm oil by GC-MS, which were most likely produced (in trace amounts) by the thermal oil rening process and are thus unlikely genuine natural products. 31 Beside tocopherols and tocotrienols, some plant species produce plastochromanol-8 (27), a g-tocochromanol with eight isoprenoid units in the side chain.…”

“…52 d-Sargachromenol (51) was found in all the above-mentioned plants and was obtained in 0.4% and 0.8% yield (dry mass) from Roldana and Iryanthera species, respectively. d-Sargachromenol is a d-dehydrotocotrienol derivative with a carboxyl group located at C-15 0 of the side chain and is thus a structurally related form of d-garcinoic acid (30) (Fig. 5).…”

“…In addition, the double bond from C-7 0 migrated to C-8 0 -C-10 0 . Further oxidation of sargachromanol L leads to sargachromanol O (68), which bears a carboxyl group at C-9 0 und is thus a structural isomer of d-garcinoic acid (30) (Fig. 8).…”

Rapid Separation of All Four Tocopherol Homologues in Selected Fruit Seeds via Supercritical Fluid Chromatography Using a Solid-Core C18 Column

“…e developed method facilitated high throughput/rapid separation of all tocopherol homologues with adequate resolution completed in a total analysis time below two min ( Figure 6). e retention factor ranged from 3.28 to 5.12 for α-T and δ-T, respectively, indicating that all are below the upper limit (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) referred as too long elution time [27].…”

Theory and Practice of UHPLC and UHPLC–MS

“…A make-up fluid for UHPSFC effluent is required for assuring good ionization efficiency of lipids (Yamada et al, 2013). Many of the SFCeMS analytical approaches from bioanalysis are also applied in food analysis for the determination of various groups of lipids and fatsoluble vitamins (Gee et al, 2016;Mejean et al, 2015;Bernal et al, 2013).…”