Vitamin D3 metabolites induce osteogenic differentiation in human dental pulp and human dental follicle cells

Khanna-Jain, Rashi; Vuorinen, Annukka; Sándor, George K.B.; Suuronen, Riitta; Miettinen, Susanna

doi:10.1016/j.jsbmb.2010.08.001

Cited by 38 publications

(43 citation statements)

References 45 publications

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“…In this study, we first sought to investigate the influence of FBS, HS and SF/XF media directly on cell isolation and proliferation. The cells isolated and cultured in FBS showed consistent proliferation, as reported in several publications [46,47]. Here, we have shown for the first time that 10% HS did not support the isolation of DPSCs, whereas, 20% HS supported the direct isolation and whereas, further expansion of the DPSCs was possible in lower HS concentrations 10% and 15%.…”

Section: Discussionsupporting

confidence: 85%

Growth and Differentiation of Human Dental Pulp Stem Cells Maintained in Fetal Bovine Serum, Human Serum and Serum-free/Xeno-free Culture Media

Khanna-Jain

Vanhatupa²,

Vuorinen³

et al. 2012

J Stem Cell Res Ther

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Introduction:Dental pulp stem cells (DPSCs) are an accessible cell source with therapeutic applicability in regeneration of damaged tissues. Current techniques for expansion of DPSCs require the use of Fetal Bovine Serum (FBS). However, animal-derived reagents stage safety issues in clinical therapy. By expanding DPSCs in serumfree/ xenofree medium (SF/XF-M) or in medium containing human serum (HS-M), the problems can be eliminated. Therefore, the aim of our study was to identify suitable cell culture media alternatives for DPSCs. Methods:We studied the isolation, proliferation, morphology, cell surface markers (CD29, CD44, CD90, CD105, CD31, CD45 and CD146), stemness markers expression (Oct3/4, Sox2, Nanog and SSEA-4) and in vitro multilineage differentiation of DPSCs in HS-M or SF/XF-M in comparison to FBS-M.Results: DPSCs expressed the cell surface and stemness markers in all studied conditions. The proliferation analysis of cells cultured in different HS concentrations revealed that cells isolated in 20% HS-M and passaged in 10% or 15% HS-M supported the cell growth. Direct isolation of cells in SF/XF-M did not support cell proliferation. Therefore, cells cultured in 20% HS-M were used for further SF/XF-M studies. However, proliferation of DPSCs was significantly lower in SF/XF-M when compared with cells cultured in FBS-M and HS-M. In addition, proliferation of DPSCs in SF/XF-M could be enhanced by addition of 1% HS in cell culture medium. There were differences in osteogenic, chondrogenic and adipogenic differentiation efficacy between cells cultured in FBS, HS and SF/XF differentation media. More pronounced adipogenic and osteogenic differentiation was observed in HS differentiation medium, however, in FBS-M cultured cells more effective chondrogenic differentiation was detected. Conclusions:Our results indicate that HS is a suitable alternative to FBS for the expansion of DPSCs. The composition of SF/XF-M needs to be further optimized in terms of cell expandability and differentiation efficiency to reach clinical applicability.

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Section: Discussionsupporting

confidence: 85%

Growth and Differentiation of Human Dental Pulp Stem Cells Maintained in Fetal Bovine Serum, Human Serum and Serum-free/Xeno-free Culture Media

Khanna-Jain

Vanhatupa²,

Vuorinen³

et al. 2012

J Stem Cell Res Ther

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“…Probably they will cause an earlier expression of some genes and enhance osteogenesis. [212930] We suggest the effect of osteogenic inducers on expression pattern of different osteoblast specific and bone-related genes will be evaluated to help researchers to choose the best osteogenic supplement for differentiation of MSCs.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic comparison of osteopontin, osteocalcin and core binding factor 1 genes between human adipose derived differentiated osteoblasts and native osteoblasts

Bahrambeigi

Salehi²,

Hashemibeni³

et al. 2012

Adv Biomed Res

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Background:There are significant limitations in repair of irrecoverable bone defects. Stem-cell therapy is a promising approach for the construction of bone tissue. Mesenchymal stem cells (MSCs) have been introduced as basic tools for bone tissue generation. Through MSCs, adipose-derived stem cells (ADSCs) are more interesting. Since the similarity of native osteoblasts and differentiated osteoblasts from ADSCs in terms of gene expression pattern is unknown, this study was designed to compare gene expression patterns of some genes involved in osteogenesis between human native osteoblasts and adipose-derived differentiated osteoblasts.Materials and Methods:Realtime qRT-PCR was used for studying the gene expression of osteocalcin, osteopontin, and core binding factor alpha 1 (Cbfa1) in human native osteoblasts and adipose derived osteogenic osteoblasts at days 7, 14, 21, and 28 of differentiation.Results:This study demonstrated that native osteoblasts and differentiated osteoblasts, cultured in common osteogenic medium, have significant differences in gene expression levels for osteocalcin and osteopontin. Compared to native osteoblasts, these genes are expressed lower in all four groups of differentiated osteoblastic cells. We also found, there is a progressive increase in cbfa1 expression over the differentiation period of ADSCs from day 7 to day 28.Conclusions:Our findings help for better assessment of adipose-derived differentiated cells as a source for cell-based therapy.

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“…Control groups were incubated in parallel with 0.1% ethanol in DMEM-10% dialyzed fetal bovine serum (Invitrogen, Carlsbad, CA, USA). The 100nM supra-physiological concentration of 1,25-D 3 applied in the experimental design was based on the present and previous dose-response studies and it is in alignment with a commonly used dose on different cell lines or in primary cell culture studies (Barbosa et al 2004, Cardus et al 2006, Artaza & Norris 2009, Artaza et al 2010, Khanna-jain et al 2010, Ramirez et al 2010). Because of the short half-life of 1,25-D 3 , the cell culture media was replaced every 24h (Garcia et al 2011, 2013).…”

Section: Methodsmentioning

confidence: 99%

1,25-Vitamin D3 promotes cardiac differentiation through modulation of the WNT signaling pathway

Hlaing¹,

Garcia²,

Contreras³

et al. 2014

Journal of Molecular Endocrinology

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Cardiovascular disease (CV) remains the leading cause of death worldwide. Low levels of vitamin D are associated with high risk of myocardial infarction, even after controlling for factors associated with coronary artery disease. A growing body of evidence suggests that vitamin D plays an important role in CV related signaling pathways. However, little is known about the molecular mechanism by which vitamin D modulates cardiac development. The Wnt signaling pathway plays a pivotal role in tissue development by controlling stem cell renewal, lineage selection and even more importantly heart development. In this study, we examined the role of 1,25-D3 (active form of vitamin D) on cardiomyocyte proliferation, apoptosis, cell phenotype, cell cycle progression and differentiation into cardiomyotubes. We determined that the addition of 1,25-D3 to cardiomyocytes cells: 1) Inhibits cell proliferation without promoting apoptosis; 2) Decreases expression of genes related to the regulation of the cell cycle; 3) Promotes cardiomyotubes formation; 4) Induces the expression of Casein kinase-1-α1, a negative regulator of the canonical Wnt signaling pathway; and 5) Increases the expression of the noncanonical Wnt11, which it has been demonstrated to induce cardiac differentiation during embryonic development in adult cells. In conclusion, we postulate that vitamin D promotes cardiac differentiation through a negative modulation of the canonical Wnt signaling pathway and by up-regulating the expression of Wnt11. These results suggest that vitamin D repletion to prevent and/or improve cardiovascular disorders that are linked to abnormal cardiac differentiation, such as post infarction cardiac remodeling, deserve further study.

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Vitamin D3 metabolites induce osteogenic differentiation in human dental pulp and human dental follicle cells

Cited by 38 publications

References 45 publications

Growth and Differentiation of Human Dental Pulp Stem Cells Maintained in Fetal Bovine Serum, Human Serum and Serum-free/Xeno-free Culture Media

Growth and Differentiation of Human Dental Pulp Stem Cells Maintained in Fetal Bovine Serum, Human Serum and Serum-free/Xeno-free Culture Media

Transcriptomic comparison of osteopontin, osteocalcin and core binding factor 1 genes between human adipose derived differentiated osteoblasts and native osteoblasts

1,25-Vitamin D3 promotes cardiac differentiation through modulation of the WNT signaling pathway

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