Vitamin D3 Administration Before and After Detorsion Could Salvage the Testicular Endocrine Function in an Experimental Model of Testicular Torsion/Detortion

Abstract

Background: Testicular torsion is one of the emergencies affecting mostly adolescent males, resulting in sub-fertility if not rapidly and efficiently managed. Oxidative stress, inflammatory response as well as immunological reactions were implicated in its pathogenesis. Recently interest in studying the non-skeletal effects of vitamin D is growing, many researches indicates its antioxidant, antiinflammatory and immuno-modulatory effects. Aim of the Work: Investigating the possible conservative effect of vitamin D 3 treatment on testicular endocrinal function in testicular torsion/detorsion rat model, and elucidating its possible underlying mechanism. Materials and Methods: 24 young adult male albino rats, weighing 120-160 g., were randomly allocated into 3 groups, 8 in each group, Sham operated (SHAM), Testicular torsion/detorsion (T/D) and Testicular torsion/detorsion; vitamin D 3 treated (T/D; D 3) groups. All rats were subjected to measurement of absolute and relative testicular weight (ATW and RTW, respectively), assessment of testicular endocrinal function by serum total testosterone and inhibin B levels. In addition to determination of serum antisperm antibody (AsAb). Testicular tissue was examined for oxidative stress markers; malondialdeyde (T. MDA) and glutathione peroxidase (T, GPx), as well as inflammatory marker; myeloperoxidase (T. MPO). Results: Testicular T/D resulted in significant reduction in ATW, RTW, serum testosterone and inhibin B levels with significant elevation in serum AsAb, T. MDA and T. MPO when compared with SHAM group. On treatment with vitamin D 3 , RTW was significantly increased as compared with T/D group but still significantly less than SHAM group. Serum testosterone level was significantly increased when compared with both SHAM and T/D groups. Inhibin B was significantly increased as compared with T/D group but still not normalized as being significantly less than SHAM group. Serum AsAb, T. MDA and T. MPO were significantly decreased when compared with T/D group, being normalized for T. MDA but still significantly higher for serum AsAb and T. MPO when compared with SHAM group, however, T. GPx was significantly increased as compared with both SHAM and T/D groups. Conclusion: Vitamin D 3 could retrieve the testicular endocrinal dysfunction induced by Torsion/Detorsion by its antioxidant, antiinflammatory and immuno-modulatory effects.

Vitamin D3 Prevents the Deleterious Effects of Testicular Torsion on Testis by Targeting miRNA-145 and ADAM17: In Silico and In Vivo Study

“…However, it was observed that unilateral testicular T/D produced pronounced injury in the ipsilateral and contralateral testis one month after detorsion, which was accompanied by significant decreases in ATW and RTW in T/D testicles, respectively. This could be attributed to a reduction of the blood supply during the torsion period [57], and to the overexpression of ROS which can damage lipids, proteins, and DNA, resulting in cellular dysfunction [58]. Our histomorphometry analysis results demonstrated that the decrease in the weight could be related to the decrease in the mean diameter of seminiferous tubules, and the mean thickness of germinal epithelium in the testicular T/D group which caused a decrease in the ATW and RTW.…”

“…It is still controversial whether unilateral testicular T/D affects contralateral testis or not, although it is obvious that the protection of non-torsioned testis plays a vital role in fertility. Oxidative stress, inflammation and immunological responses have been implicated in the pathogenesis of contralateral testicular affection after unilateral testicular T/D [57]. In the current study, we investigated the pathogenesis of contralateral testicular affection after unilateral testicular T/D and the possible role of vitamin D3 on ipsilateral and contralateral testicular injury.…”