Vitamin D receptor gene polymorphisms in Sudanese children with type 1 diabetes

Khalid, Khalid Eltahir

doi:10.3934/genet.2016.3.167

Cited by 13 publications

(3 citation statements)

References 37 publications

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“…This high variability and rapidly increased incidence of T1DM in various world populations could be owing to differences in ethnic background and nationality. These 4 VDR gene polymorphisms were examined for their connection with T1DM in a variety of populations, such as JApan, India, Kuwait, Iran, Turkish, and Finland, with mixed results (16,17). A South Indian analysis revealed that the b-allele of the VDR gene, BsmI polymorphism, was linked to T1DM (18).…”

Section: Resultsmentioning

confidence: 99%

VDR Gene Polymorphisms in Kurdish Population and Its Relation to T1DM in Erbil-Iraq

Othman

2022

Cell Mol Biol (Noisy-le-grand)

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This research assessed the relationship among type 1 diabetes VDR gene polymorphisms (ApaI and TaqI) in the Kurdish population in Erbil-Iraq. Forty individuals with type 1 diabetes and thirty healthy people were recruited from the Kurdish population in Erbil, Iraq. Genomic DNA was taken from blood, being genotyped for SNP (single nucleotide polymorphisms). The distribution of VDR polymorphisms in two restriction fragment length polymorphism sites, TaqI and ApaI, was investigated in patients and controlled by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) utilizing ApaI and TaqI restriction enzymes. Using SPSS software (V15.0), the genotype dispersal and allelic incidences in patients and controls were compared. VDR polymorphism genotype dispersal and allele incidences vary dramatically among patients and controls. The results confirmed that the genotype GT in SNP ApaI was a risk factor among type 1 diabetes mellitus patients' combination that imparted the strongest susceptibility to T1DM (P=0.00023). Still, the SNP TaqI showed no relevance between cases and controls (P=0.35). Our findings indicate that VDR gene polymorphisms in the combination of genotypes are related to an increased risk of T1DM in the Kurdish community and warrant further investigation as a possible genetic risk marker for T1DM. More research is needed to corroborate this finding, particularly the VDR gene, which was studied for the first time in the Kurdish population.

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Section: Resultsmentioning

confidence: 99%

VDR Gene Polymorphisms in Kurdish Population and Its Relation to T1DM in Erbil-Iraq

Othman

2022

Cell Mol Biol (Noisy-le-grand)

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“…In contrast, no significant association between three VDR gene polymorphisms ( Bsm I, Apa I and Taq I) and T1DM was detected in patients from Chile [27]. The data from Sudan, showed an association of two VDR gene polymorphisms ( Bsm I and Taq I) with T1DM [28]. In a study from Pakistan, VDR gene polymorphisms Fok I and Apa I were found to be associated with T1DM while no association was found with the Taq I polymorphism [29].…”

Section: Introductionmentioning

confidence: 99%

Relationship of four vitamin D receptor gene polymorphisms with type 1 diabetes mellitus susceptibility in Kuwaiti children

et al. 2019

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Background The incidence of type 1 diabetes mellitus (T1DM) in Kuwait is amongst the highest in the world. Vitamin D is considered to be involved in immune modulation and its deficiency contribute to autoimmune destruction of insulin producing beta cells in T1DM patients. Vitamin D has been shown to exert its effects via a nuclear vitamin D receptor (VDR) and therefore, VDR gene may be considered a candidate for T1DM susceptibility. Methods The genotypes of four VDR gene polymorphisms were determined in 253 Kuwaiti Arab T1DM patients and 214 healthy controls by PCR-RFLP analysis. Serum concentrations of three autoantibodies i.e. ICA (Islet cell autoantibody), GADA (Glutamic acid decarboxylase) and INS (Insulin autoantibody) were determined by radio-immunoassays. Results Statistically significant differences were detected between the genotypes of two VDR gene polymorphisms ( Fok I, C > T, rs10735810 and Taq I, C > T, rs731236) between T1DM patients and controls ( P < 0.0001). In both, the frequency of variant alleles was considerably high in T1DM than in the controls. In contrast, the VDR gene Apa I (G > T, rs7975232) and Bsm I (A > G, rs1544410) polymorphisms did not show association with T1DM. The homozygous variant genotypes of Fok I, Apa I and Taq I polymorphisms show significant differences between various age-of-onset subgroups while no such association was detected in the case of Bsm I polymorphism. Significant differences were also noted between heterozygous genotypes of all four polymorphisms especially between 4-6y and > 6y age-of-onset subgroups of T1DM patients. Three autoantibodies, ICA (Islet cell), GADA (glutamate decarboxylase) and INS (insulin) were positively associated to, varying degrees, with T1DM in Kuwaiti Arabs harboring different VDR gene polymorphism genotypes. Conclusions Our results demonstrate a significant effect of two VDR gene polymorphisms ( Fok I and Taq I) and three autoantibodies on genetic susceptibility of T1DM in Kuwaiti Arabs along with other factors.

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“…The primers of the VDR gene were designed based on previous literature. 19 The PCR condition used for Bsm I as followed: an initial denaturation of 3 min at 94 °C, followed by denaturation of 30 s at 62 °C, and extension of 1 min at 72 °C for 30 cycles, and a final extension of 5 min at 72 °C. The PCR condition used for amplification of Apa1 as follows; 94 °C for 10 min, and 30 cycles using the following temperature profile: 94 °C for 1 min, 62 °C for 1 min, 72 °C for 1 min, and final elongation for 5 min.…”

Section: Genotyping Of Vdr Gene Polymorphic Variantsmentioning

confidence: 99%

The Associations Between Vitamin D Receptor BsmI and ApaI Polymorphisms and Obesity in Korean Patients with Type 2 Diabetes Mellitus

Nam¹,

Choi²,

Jeon³

et al. 2021

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Background: Vitamin D receptor (VDR) polymorphisms are associated with osteoporosis, diabetes, immunological diseases, and cancers. However, the association of obesity with VDR polymorphisms has shown inconsistent results, and perhaps it depends upon the characteristics of a population. Therefore, we evaluated the association between BsmI (rs1544410) and ApaI (rs7975232) polymorphisms of VDR and obesity in Korean patients with type 2 diabetes mellitus (T2DM). Methods: A total of 506 patients with T2DM participated in the study. Polymerase chain reaction-restriction fragment length polymorphism was used to analyze BsmI and ApaI polymorphisms; the genotypes were presented as BB, Bb, or bb for BsmI and AA, Aa, or aa for ApaI. Obesity was defined using the body mass index (BMI) with a cutoff level of 25 kg/m 2. Results: The prevalence of obesity was higher in patients with the bb genotype than in those with BB or Bb genotypes (48.4% vs 33.9%, P = 0.031). The mean BMI was 25.2 ± 3.5 kg/m 2 in patients with bb genotype and 24.1 ± 3.1 kg/m 2 in patients with BB or Bb genotypes. Patients with Aa or aa genotypes showed a higher prevalence of obesity than patients with AA genotype (47.6% vs 26.1%, P = 0.043). Glycemic control parameters and lipid profiles did not show significant differences with either polymorphism. Conclusion: To our knowledge, this is the first study to assess the association between VDR polymorphisms and obesity in Korean patients with T2DM. Further studies in larger populations and multiethnic cohorts are needed to validate our findings.

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Vitamin D receptor gene polymorphisms in Sudanese children with type 1 diabetes

Cited by 13 publications

References 37 publications

VDR Gene Polymorphisms in Kurdish Population and Its Relation to T1DM in Erbil-Iraq

VDR Gene Polymorphisms in Kurdish Population and Its Relation to T1DM in Erbil-Iraq

Relationship of four vitamin D receptor gene polymorphisms with type 1 diabetes mellitus susceptibility in Kuwaiti children

The Associations Between Vitamin D Receptor BsmI and ApaI Polymorphisms and Obesity in Korean Patients with Type 2 Diabetes Mellitus

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