Vitamin D Receptor Gene Polymorphism ApaI as a Predisposing Factor for Psoriasis and Its Relation With Serum Vitamin D Levels and Psoriasis Severity

Background and aimsPsoriasis is a chronic, non‐contagious autoimmune condition marked by dry, itchy,erythematous and scaly plaques. From modest, localized plaques to total body coverage, the severity of psoriasis varies. Plaque, guttate, inverted, pustular, and erythrodermic psoriasis are the five primary kinds. About 90% of cases are of plaque psoriasis, commonly known as psoriasis vulgaris. Study aims to determine the impact of an rs2228570 (FokI) variant and an rs11568820 (CDX2) variant on serum vitamin D levels (SVD) in patients with psoriasis, and the correlation between the two variants and disease severity.MethodsA case‐control study consisting of 95 psoriasis vulgaris patients and 84 healthy controls. The clinical investigation, molecular genetics analysis, and biochemical analysis were done for both groups.ResultsSVD levels were significantly decreased in psoriasis patients group. FokI genotypes analysis, we found no significant variance between groups. CDX2 G/G genotype is more prevalent in patients than controls. Moderate psoriasis vulgaris patients with CDX2 G/G genotypes have higher SVD levels than CDX2 G/A, and CDX2 A/A p = 0.003.ConclusionThe study found a difference in vitamin D levels between patients and healthy subjects, as well as a difference in vitamin D levels with different FoKI and CDX2 genotypes.

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

“…Previously, we examined TaqI 13 and ApaI 14 . This study carried on to ascertain how other variations in the VDR genes FoKI and CDX2 affect the chance of developing psoriasis.…”

Section: Introductionmentioning

confidence: 99%

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The CDX2 G allele and the FoKI F allele of the VDR gene are more prevalent and related to changes in vitamin D levels in patients with psoriasis vulgaris: A pilot study

AbdElneam,

Al‐Dhubaibi,

Bahaj

et al. 2023

“…14 In our population, several studies have been conducted to understand the relationship between genetic factors and dermatological conditions. [15][16][17][18][19][20][21][22][23][24] However, according to our knowledge, no study tries to determine the mechanisms by which miRNAs contribute to AA.…”

Section: Introductionmentioning

confidence: 99%

MiR‐200c‐3p as a novel genetic marker and therapeutic tool for alopecia areata

AbdElneam,

Al‐Dhubaibi,

Bahaj

et al. 2024

Self Cite

BackgroundMicroRNAs (miRNAs) are small RNA molecules that regulate gene expression in diverse biological processes. They hold promise as therapeutic candidates for targeting human disease pathways, although our understanding of their gene regulatory mechanism remains incomplete. Alopecia areata (AA) is a prevalent inflammatory ailment distinguished by the infiltration of T cells targeting the anagen‐stage hair follicles. The scarcity of effective remedies for AA may stem from limited understanding regarding its precise cellular mechanism.AimTo investigate and examine the importance and role of the miR‐200c‐3p as a genetic indicator for AA, and its possible impact on disease progression.Subjects and methodsCase‐control study included 65 patients with AA and 65 matched healthy controls. A real‐time PCR technique was used to measure the expression of miR‐200c‐3p for both groups. Bioinformatic tools were used for prediction with genes and gene‐gene interaction, and protein‐protein interaction.ResultsThe expression levels of miR‐200c‐3p were significantly higher in AA patients than in healthy controls. We predicted that miR‐200c‐3p plays a markable role in the development of AA by its effect on the EGFR tyrosine kinase inhibitor resistance pathway.ConclusionWe were able to identify the influence of miR‐200c‐3p on both PLCG1 and RPS6KP1 genes which in turn regulate the EGFR tyrosine kinases resistance pathway that displayed the most substantial increase in activity. Our outcomes shed light on the era of the potential theranostic role of this innovative miRNA in AA.

C‐reactive protein as a novel biomarker for vitamin D deficiency in alopecia areata

AbdElneam,

Al‐Dhubaibi,

Bahaj

et al. 2024

BackgroundAlopecia areata (AA) is an autoimmune condition characterized by sudden and unpredictable hair loss, with a lifetime incidence of 2%. AA can be divided into three categories: patchy alopecia, alopecia totalis, and alopecia universalis. It can affect a person's psychological health and overall quality of life. Elevated C‐reactive protein (CRP) levels in the liver may indicate an inflammatory response in autoimmune diseases. Vitamin D, essential for immune system control and skin health, may be related to AA. Hair follicles contain vitamin D receptors, which control immunological responses in the skin. However, no study has found a relationship between CRP and vitamin D in AA patients in our region.Subjects and MethodsAn analytical cross‐sectional study with a case‐control design research investigation of 82 AA patients and 81 healthy controls was carried out. Both groups’ medical histories were taken. Biochemical analysis was done for both groups as well as the serum vitamin D levels, and CRP. Genetic analysis for CDX2 rs11568820 variant detected by PCR (T‐ARMS‐PCR) method and vitamin D receptor (VDR) gene expression measured by real‐time PCR analysis for both patients and healthy subjects.ResultsCRP levels are higher in AA patients, AA patients with G/G genotypes exhibited higher concentrations of CRP when compared to those with A/A and A/G genotypes while patients with A/A genotypes have higher levels of Serum vitamin D as compared to the A/G and G/G genotypes. G allele was more abundant in AA patients. VDR gene expression was lower in AA compared to control and lower in ophiasis compared to localized and multiple patchy AA. An important inverse linear correlation was observed between vitamin D and CRP levels in ophiasis AA.ConclusionCRP concentrations were found to be elevated in AA patients. The considerable accuracy of CRP in the diagnosis of AA is substantiated by a statistically significant al. A noteworthy inverse linear association was observed between serum vitamin D and CRP concentrations in ophiasis AA.