Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells

Yao, Xiaolei; Zhang, Guomin; Guo, Yanliang; EI-Samahy, M.A.; Wang, Zhen; Wan, Yongjie; Han, Le; Liu, Zifei; Zhang, Yanli

doi:10.1016/j.theriogenology.2017.08.002

Cited by 59 publications

(43 citation statements)

References 43 publications

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“…Other than the current study, oocyte VDR expression has only been reported in fish ( Bidman et al, 1997 ; Craig et al, 2008 ). In contrast, VDR expression in granulosa cells was noted in various species, including rodents, domestic animals, and humans ( Johnson et al, 1996 ; Thill et al, 2009 ; Yao et al, 2017 ). The current data suggest the positive impact of VD3 on preantral follicle survival, as well as on both survival and growth of antral follicles developed in vitro .…”

Section: Discussionmentioning

confidence: 99%

“…Recent research revealed that VD3 may exert its effect by altering reactive oxygen species production. VD3 addition decreased reactive oxygen species levels in cultured goat granulosa cells ( Yao et al, 2017 ). An increase in plasma total antioxidant capacity was observed following vitamin D-K-calcium co-supplementation in vitamin D-deficient patients with polycystic ovary syndrome ( Razavi et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…VD3 treatment was also reported to decreased caspase-3 activity in cultured human umbilical vein cell line ( Ma et al, 2017 ). Thus, future studies need to further elucidate the role of vitamin D in maintaining and/or enhancing follicle health during early folliculogenesis, which could be through its antioxidant or anti-apoptotic actions, as well as by promoting granulosa cell proliferation and follicle growth during later stages of follicular development ( Xu et al, 2016b ; Yao et al, 2017 ). Approaches may include assessing expression of pro- and anti-apoptotic genes, as well as evaluating cell proliferation using bromodeoxyuridine as a marker ( Ting et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

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Vitamin D3 Regulates Follicular Development and Intrafollicular Vitamin D Biosynthesis and Signaling in the Primate Ovary

Lawson

et al. 2018

Front. Physiol.

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There is an increasing recognition that vitamin D plays important roles in female reproduction. Recent studies demonstrated that 1α,25-dihydroxyvitamin D3 (VD3), the biologically active form of vitamin D, improved ovarian follicle survival and growth in vitro. Therefore, we investigated the direct effects of VD3 at the specific preantral and antral stages of follicular development, and tested the hypothesis that vitamin D receptor (VDR) and enzymes critical for vitamin D biosynthesis are expressed in the primate ovary. Fourteen adult rhesus macaques provided ovarian tissue. Secondary and antral follicles were isolated for PCR analysis on VDR, vitamin D3 25-hydroxylase, and 25-hydroxyvitamin D3-1α-hydroxylase. VDR protein localization was determined by immunohistochemistry on ovarian sections. Isolated secondary follicles were cultured under conditions of control and VD3 supplementation during the preantral or antral stage. Follicle survival, growth, steroid and anti-Müllerian hormone (AMH) production, as well as oocyte maturation were evaluated. In vivo- and in vitro-developed follicles were also assessed for genes that are critical for vitamin D biosynthesis and signaling, gonadotropin signaling, steroid and paracrine factor production, and oocyte quality. The mRNA encoding VDR, 25-hydroxylase, and 1α-hydroxylase was detectable in in vivo- and in vitro-developed preantral and antral follicles. The 25-hydroxylase was elevated in cultured follicles relative to in vivo-developed follicles, which further increased following VD3 exposure. VD3 treatment increased 1α-hydroxylase in in vitro-developed antral follicles. The absence of VD3 during culture decreased VDR expression in in vitro-developed antral follicles, which was restored to levels comparable to those of in vivo-developed antral follicles by VD3 supplementation. Positive immunostaining for VDR was detected in the nucleus and cytoplasm of granulosa cells and oocytes. While only survival was improved in preantral follicles treated with VD3, VD3 supplementation promoted both survival and growth of antral follicles with increased estradiol and AMH production, as well as oocyte maturation. Thus, Vitamin D biosynthesis and signaling systems are expressed in primate ovarian follicles. Our findings support a role for VD3 in regulating follicular development in a stage-dependent manner, as well as the intrafollicular vitamin D biosynthesis and signaling, directly in the ovary.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Vitamin D3 Regulates Follicular Development and Intrafollicular Vitamin D Biosynthesis and Signaling in the Primate Ovary

Lawson

et al. 2018

Front. Physiol.

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“…Vitamin D3 has been shown to play an important role in ovulation dysfunction in mice and may be mediated by anti‐Mullerian hormone (AMH) produced by FGCs in the ovary, thereby inhibiting follicle death which contain oocytes (Carlsson et al, ; Davis, Merhi, Tolga‐Suntay, & Neal‐Perry, ). Yao et al () studied the effects of vitamin D3 on the function of goat FGCs and found that the vitamin played important roles in the proliferation of granulosa cells by regulating oxidative stress and cell cycle‐related genes (Yao et al, ). These studies demonstrated that vitamin D3 plays an important role in FGC development.…”

Section: Discussionmentioning

confidence: 99%

Metabonomics analysis of Zi goose follicular granulosa cells using ENO1 gene expression interference

Guo

Niu

et al. 2019

Animal Physiology Nutrition

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The Zi goose is native to North‐east China and is noted for its high egg production. Alpha enolase (ENO1) is a glycolytic enzyme which functions as a plasminogen receptor in follicular granulosa cells (FGCs), with several studies showing that FGCs can support follicular development. By transfecting the ENO1 interfering plasmid (shRNA) into FGCs, ENO1 expression in these cells was downregulated, suggesting the successful knock‐down of ENO1 in these cells. In this knock‐down model, we detected 13 metabolites from FGCs using LC/MS. When compared with the non‐coding shRNA (NC) group, the lower level metabolites were (R)‐(+)‐citronellic acid, altretamine, 3‐hydroxycaproic acid, heptadecanoic acid, cholecalciferol vitamin D3, indole, benzoic acid, capric acid, caffeic acid, azelaic acid, 3,4‐dihydroxyhydrocinnamic acid and cholic acid, while oleic acid was detected at high levels. To further examine the results of metabolomics, six key metabolites were verified by gas chromatography‐mass spectrometry (GC‐MS). We found that vitamin D3, indole, benzoic acid, capric acid and cholic acid were significantly downregulated in the shRNA group, while oleic acid was significantly upregulated. This observation was consistent with the metabolomics data. Through these studies, we found that decreased ENO1 levels altered certain metabolite levels in FGCs.

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“…Moreover, studies done on mice with PCOS, found that vitamin D can modulate steroidogenesis in GCs, through activating AMP activated protein kinase (AMPK) signaling pathway, and thus improves follicle maturation in PCOS (37,38). Vitamin D might regulate cellular oxidative stress in GCs, and thus may have an essential role in follicle development (39). These findings might give an explanation of the role of vitamin D in oocyte maturation.…”

Section: Methodsmentioning

confidence: 97%

Level of follicular fluid vitamin D and embryo quality in a sample of Iraqi women undergoing IVF

Alawad

2019

JFacMedBagdad

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Background: Vitamin D has been linked to fertility since its receptors were found in reproductive organs. Nevertheless, its exact role in fertility is yet to be cleared. Objectives: To analyze follicular fluid (FF) vitamin D in females doing Intracytoplasmic Sperm Injection (ICSI), to compare its level between Polycystic Ovary Syndrome (PCOS) and non-PCOS females, and to explore whether vitamin D correlates with oocytes number, percentage of oocyte maturity, fertilization rate and percentage of high quality embryos. Patients and methods: This comparative observational study was done, from January to October 2018, at Al Amal IVF Center, Jenin hospital, Baghdad. The study was conducted on 40 women; 18 had PCOS, 15 had blocked fallopian tubes and 7 had idiopathic infertility. FF vitamin D was analyzed by Ichroma kit, Boditech. Deficient, insufficient, and sufficient vitamin D levels were as: <10, 10-29, and 30-100 ng/ml, respectively. Results: Most participants were deficient (12 women) or insufficient (18 women) of vitamin D, only 10 women had adequate vitamin D levels. FF vitamin D was higher in non-PCOS than PCOS women (P= 0.011). The correlation between vitamin D and oocytes number was not significant. However, it was significant with percentage of oocyte maturity (rho= 0.459, P= 0.003), fertilization rate (rho= 0.471, P= 0.002) and percentage of high quality embryos (rho= 0.316, P= 0.047). Conclusions: PCOS patients, when compared to non-PCOS, have lower vitamin D levels. FF vitamin D might correlate with percentage of oocyte maturity, fertilization rate and percentage of high quality embryos in patients undergoing ICSI.

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Vitamin D receptor expression and potential role of vitamin D on cell proliferation and steroidogenesis in goat ovarian granulosa cells

Cited by 59 publications

References 43 publications

Vitamin D3 Regulates Follicular Development and Intrafollicular Vitamin D Biosynthesis and Signaling in the Primate Ovary

Vitamin D3 Regulates Follicular Development and Intrafollicular Vitamin D Biosynthesis and Signaling in the Primate Ovary

Metabonomics analysis of Zi goose follicular granulosa cells using ENO1 gene expression interference

Level of follicular fluid vitamin D and embryo quality in a sample of Iraqi women undergoing IVF

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