Vitamin D promotes growth, feed utilization and gene expression related to lipid metabolism for juvenile Orange-spotted grouper Epinephelus coioides

Lin, Huaxing; Ding, Mao; Ray, Gyan Watson; Tan, Beiping; Yang, Qihui

doi:10.1016/j.aqrep.2022.101074

Cited by 7 publications

(3 citation statements)

References 36 publications

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“…After the breeding experiment, groupers were starved for 24 h before samples were collected [30]. All fish were anesthetized with MS-222 before the sample collection.…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of Plant-Derived Glycerol Monolaurate (GML) Additive on the Antioxidant Capacity, Anti-Inflammatory Ability, Muscle Nutritional Value, and Intestinal Flora of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)

et al. 2022

Metabolites

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In a context where the search for plant-derived additives is a hot topic, glycerol monolaurate (GML) was chosen as our subject to study its effect on grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂). Seven gradient levels of GML (0, 600, 1200, 1800, 2400, 3000, and 3600 mg/kg) were used for the experiment. Based on our experiments, 1800 mg/kg GML significantly increased the final body weight (FBW) and weight gain rate (WGR). GML increased the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and decreased malondialdehyde (MDA). Adding 1800 mg/kg GML also significantly increased the levels of lauric acid (C12:0) (LA), n-3 polyunsaturated fatty acids (PFA), and the n-6 PFA-to-n-3/n-6 ratio, while significantly decreasing the levels of saturated fatty acids (SFA). Dietary supplementation with GML significantly inhibited the expression of pro-inflammatory factors and reduced the occurrence of inflammation. GML improved intestinal flora and the abundance of beneficial bacteria (Bacillus, Psychrobacter, Acinetobacter, Acinetobacter, Stenotrophomonas, and Glutamicibacter). It provides a theoretical basis for the application of GML in aquafeed and greatly enhances the possibility of using GML in aquafeed. Based on the above experimental results, the optimum level of GML in grouper feed is 1800 mg/kg.

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“…After the breeding experiment, groupers were starved for 24 h before samples were collected [30]. All fish were anesthetized with MS-222 before the sample collection.…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of Plant-Derived Glycerol Monolaurate (GML) Additive on the Antioxidant Capacity, Anti-Inflammatory Ability, Muscle Nutritional Value, and Intestinal Flora of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)

et al. 2022

Metabolites

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“…After blood collection, muscle and liver of three fish randomly selected from each floating cage were stored frozen at -20°C to detect the composition. Furthermore, the liver samples of six randomly selected fish each floating cage were quickly removed, one part (three fish) was stored for enzymes activity analysis, whereas the others (three fish) were stored in RNA-later (Ambion, Texas, America) at -80°C before RNA isolation (Lin et al, 2022).…”

Section: Sample Collectionmentioning

confidence: 99%

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

et al. 2022

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Glycerol monolaurate (GML), a representative fatty acid glyceride, was used to promote growth. This study investigated the effects of GML in diets on growth, serum biochemical parameters, antioxidant capacity, liver morphology and lipid metabolism for juvenile pompano Trachinotus ovatus. Six groups of T. ovatus (mean weight = 14.00 ± 0.70 g) were fed with six diets, containing varying GML levels of 0.00% (G0), 0.05% (G5), 0.10% (G10), 0.15% (G15), 0.20% (G20), and 0.25% (G25) for 8 weeks. Fish were reared for in floating cages (length: 1 m; width: 1 m; and height: 2 m). The highest weight gain rate (WGR), specific growth rate (SGR), and protein deposit rate (PDR) were observed in the G15 group (P < 0.05). Hepatic glutathione peroxidase, total antioxidant capacity and superoxide dismutase activities were significantly increased in the G10 and G15 groups compared with the G0 group (P < 0.05), whereas hepatic malondialdehyde (MDA) was significantly reduced in the G10 and G15 groups (P < 0.05). The alkaline phosphatase activity was significantly higher in the G5, G10, G15, and G20 groups than in the G0 group (P < 0.05), and the highest AKP activity was observed in the G15 group. Alanine aminotransferase (ALT) activity was significantly lower in the G10, G15, G20, and G25 groups than in the G0 group (P < 0.05). The addition of GML to the feed significantly reduced triglyceride (TG) content (P < 0.05). In comparison with the G0 group, the G15, G20, and G25 groups had significantly lower total cholesterol content and significantly higher high-density lipoprotein content (P < 0.05). Low-density lipoprotein content was significantly lower in the G5 and G10 groups than in the G0 group (P < 0.05). Serious vacuolation occurred in the G0 group, but the cell boundaries in all added groups were obvious, and the rate of intracellular vacuolization decreased. As the GML level increased, carnitine palmitoyl-transferase Ι mRNA level was significantly up-regulated (P < 0.05). The expression of fatty acid synthesis in the G10, G15, G20, and G25 groups was significantly lower than that in the G0 group (P < 0.05). The expression of sterol regulatory element-binding protein-Ι was significantly lower in the G10, G15, G20, and G25 groups than in the G0 group (P < 0.05). Based on the broken-line model of WGR and PDR corresponding to GML levels, the optimum addition level of dietary GML was 0.14%-0.16% for juvenile T. ovatus. In conclusion, 0.15% GML significantly improved the growth performance, hepatic antioxidant ability, and lipid metabolism and protected the liver for juvenile pompano T. ovatus.

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“…The shrimp samples of each experimental group were calculated and weighed, and it was determined the weight gain rate, survival rate, specific growth rate, and feed coefficient rate ( 33 ). Afterward, three shrimp samples per tank were randomly selected to store at −20°C to detect the whole-body composition ( 34 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of partially replacing fishmeal with corn gluten meal on growth, feed utilization, digestive enzyme activity, and apparent nutrient digestibility for juvenile white shrimp, Litopenaeus vannamei

et al. 2023

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This experiment was conducted to assess the possibility of replacing fishmeal (FM, Fishmeal content of the control group: 30%) with corn gluten meal (CGM) at the following levels: 0, 10, 20, 30, 40, and 60%. The experimental diets, formulated to be isonitrogenous and isocaloric, were studied for their effects on growth, feed utilization, digestive enzyme activity and apparent nutrient digestibility in juvenile white shrimps, Litopenaeus vannamei (initial mean weight = 0.71 ± 0.01 g). Seven hundred twenty healthy and uniformed-size shrimp were distributed to six groups of three replicates, each with 40 shrimp in each tank (0.5 m3). Each experimental diet was fed to shrimp four times daily to apparent satiation at 7:00, 11:30, 17:00, and 21:30, respectively, for 8 weeks. At the end of the experiment, the total weight of fish in each tank was weighed and randomly selected for testing, including fish nutrient composition and digestive enzyme activity. Results showed that no significant differences were observed in the weight gain rate (WGR), feed coefficient rate (FCR) and specific growth rate (SGR) of shrimp after 30% FM was replaced with CGM (P > 0.05), but these indicators significantly decreased at higher replacement rates. As CGM content increased, the content of crude protein and phosphorus in the shrimp decreased significantly (P < 0.05), whereas the crude fat content first increased significantly and then decreased (P < 0.05). Compared to the control group, the protease activity was significantly lower in the 40% group and the lipase activity was significantly lower in the 60% group (P < 0.05). Amylase activity was significantly increased with increasing CGM levels (P < 0.05). The digestibility of protein and lipid was significantly reduced by CGM replacement of more than 30% FM (P < 0.05). As CGM content increased, the digestion of energy and dry matter was first significantly increased and then significantly decreased (P < 0.05). In the 30, 40, and 60% groups, the digestibility of all amino acids (AA), except methionine (Met), arginine (Arg) and serine (Ser), was significantly lower than in the control group (P < 0.05). In summary, FM could be partially replaced by CGM in the feed of L. vannamei. Based on the broken-line regression analysis of WGR, the optimal dietary CGM replacement was 27.47%.

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Vitamin D promotes growth, feed utilization and gene expression related to lipid metabolism for juvenile Orange-spotted grouper Epinephelus coioides

Cited by 7 publications

References 36 publications

Effects of Plant-Derived Glycerol Monolaurate (GML) Additive on the Antioxidant Capacity, Anti-Inflammatory Ability, Muscle Nutritional Value, and Intestinal Flora of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)

Effects of Plant-Derived Glycerol Monolaurate (GML) Additive on the Antioxidant Capacity, Anti-Inflammatory Ability, Muscle Nutritional Value, and Intestinal Flora of Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂)

Effects of Dietary Glycerol Monolaurate on Growth, Antioxidant Capacity and Lipid Metabolism in Cage-Farmed Pompano (Trachinotus ovatus) Juveniles

Effects of partially replacing fishmeal with corn gluten meal on growth, feed utilization, digestive enzyme activity, and apparent nutrient digestibility for juvenile white shrimp, Litopenaeus vannamei

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