Vitamin D Modulates Hematological Parameters and Cell Migration into Peritoneal and Pulmonary Cavities in Alloxan-Diabetic Mice

Abstract

Background/Aims. The effects of cholecalciferol supplementation on the course of diabetes in humans and animals need to be better understood. Therefore, this study investigated the effect of short-term cholecalciferol supplementation on biochemical and hematological parameters in mice. Methods. Male diabetic (alloxan, 60 mg/kg i.v., 10 days) and nondiabetic mice were supplemented with cholecalciferol for seven days. The following parameters were determined: serum levels of 25-hydroxyvitamin D, phosphorus, calcium, urea, creatinine, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, red blood cell count, white blood cell count (WBC), hematocrit, hemoglobin, differential cell counts of peritoneal lavage (PeL), and bronchoalveolar lavage (BAL) fluids and morphological analysis of lung, kidney, and liver tissues. Results. Relative to controls, cholecalciferol supplementation increased serum levels of 25-hydroxyvitamin D, calcium, hemoglobin, hematocrit, and red blood cell counts and decreased leukocyte cell counts of PeL and BAL fluids in diabetic mice. Diabetic mice that were not treated with cholecalciferol had lower serum calcium and albumin levels and hemoglobin, WBC, and mononuclear blood cell counts and higher serum creatinine and urea levels than controls. Conclusion. Our results suggest that cholecalciferol supplementation improves the hematological parameters and reduces leukocyte migration into the PeL and BAL lavage of diabetic mice.

Radioprotective effect of lactoferrin in mice exposed to sublethal X‑ray irradiation

“…In addition, the leukocyte count appears to stay steady between days 9 and 14 in the IR+LF group at ~2.6x10 3 /µl. On day 29 the leukocyte count of the IR+LF mice had stabilized to within the normal range (7.6x10 9 -10.9x10 9 /l) (21). The significant difference was identified between the control group and the IR+LF group except on day 29 (P<0.05).…”

“…Following a decrease post irradiation, the PLT count in the IR+LF group was restored to within a normal range on day 19 (Fig. 5) (21). However, in the IR group, the PLT count decreased to a minimum value at day 9 and slowly increased to a normal level (21) by day 29.…”

“…The measurements included leukocyte, erythrocyte and platelet (PLT) counts, as well the hemoglobin. The normal references value of hematological parameters were described previously (21).…”

Colecalciferol regula os parâmetros hematológicos e a produção de citocinas pró-inflamatórias renais em camundongos diabéticos e nas células RAW 264.7

“…Após esse período experimental, os animais do grupo controle exibiram ganho de peso corporal, bem como normoglicemia. Entretanto, os animais tornados diabéticos exibiram redução do peso corporal e hiperglicemia, caracterizando o estado insulinopênico (BELLA et al, 2017). A hiperglicemia crônica pode elevar a lipólise e degradar aminoácidos, aumentando o gasto energético dos tecidos, o que explica a perda de peso corporal nos animais diabéticos (OLIVEIRA et al, 2013;CHAMBERLAIN et al, 2016).…”

“…Uma das estratégias para avaliar a anemia, muito comum em indivíduos com DM1, é a avaliação da concentração sérica de hemoglobina (COLAK et al, 2014). Recentemente, nosso grupo observou que camundongos diabéticos suplementados com 800UI/dia de colecalciferol, quando comparados aos camundongos diabéticos, exibiram aumento na hemoglobina, na hematimetria e no hematócrito (BELLA et al, 2017). Uma possível justificativa para esses resultados seria a interação da vitamina D com o VDR presente nas células da medula óssea (ZHOU; LEBOFF; GLOWACKI, 2010).…”