Vitamin D: Correlation with biochemical and body composition changes in a southern Brazilian population and induction of cytotoxicity in mesenchymal stem cells derived from human adipose tissue

Abstract

Studies have shown that metabolic disorders, serum inflammatory markers and weight gain (obesity) are correlated with vitamin D deficiency. Therefore, the present study correlated the serum calcidiol (s25(OH)D) levels in a sample of individuals from southern Brazil with variables related to metabolic disorders, obesity and lifestyle habits and assessed the cytotoxic effect of calcitriol on adipose tissue-derived mesenchymal stem cells (ADSCs). The results showed a 79.23% prevalence of hypovitaminosis D in the study population and a correlation (p<0.05) between a low serum vitamin D concentration and an elevated low-density lipoprotein cholesterol (LDL-c) level. Univariate linear regression analysis using 25(OH)D as a regressor showed a negative association (p<0.05) with an indoor work environment (β=-2.305), increased body fat (β=-0.095), age (β=-0.065) and high-density lipoprotein cholesterol (HDL-c; β=-0.109). An in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay performed with ADSCs using five calcitriol concentrations (15.625, 31.25, 62.5, 125 and 250nM) indicated cytotoxic potential (p<0.05) at the 62.5nM concentration at 48 and 72h and at the 125 and 250nM concentrations at 24, 48 and 72h. The results reported herein corroborate one another and suggest a key association between vitamin D deficiency and the development of obesity because ADSCs are involved in adipose tissue hyperplasia and differentiate into adipocytes that can sequester the bioavailable vitamin D necessary for homeostasis.

Factors Associated with Bone Health in Malaysian Middle-Aged and Elderly Women Assessed via Quantitative Ultrasound

“…Vitamin D, an important nutrient for bone health, is often reported to be low in obese individuals [ 47 ]. This could be due to the lack of physical activity and sunlight exposure, or the sequestration of vitamin D by adipose tissue, rendering it unavailable for bone homeostasis [ 48 , 49 ]. These could explain the negative association between fat mass and bone in this study.…”

Evaluation of the Antitumor Potential of the Resorcinolic Lipid 3-Heptyl-3,4,6-trimethoxy-3H-isobenzofuran-1-one in Breast Cancer Cells

“…Cell viability assay. The cytotoxic potential of AMS35AA was evaluated by the MTT colorimetric test performed as described by Pesarini et al (2017) and Schweich et al (2017), with modifications (6, 7). Cells were seeded at a density of 3×10 3 in 96 well culture plates in a supplemented DMEM medium and incubated in 5% CO 2 atmosphere at 37˚C for 24 h for adherence and stabilization.…”

The Role of Vitamin D in Modulating Mesenchymal Stem Cells and Endothelial Progenitor Cells for Vascular Calcification

“…Thus, adipose tissue-derived MSCs can be modulated by vitamin D, especially nutritional vitamin D (e.g., cholecalciferol). From the study of Pesarini et al, vitamin D decreased the viability in time-and dose-dependent manners on the adipose tissue-derived MSCs and decreased the further adipose tissue formation [150].…”