Vitamin D and Vitamin D Binding Protein in Health and Disease 2.0

Delrue, Charlotte; Speeckaert, Marijn M.

doi:10.3390/ijms241210316

Cited by 2 publications

(2 citation statements)

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“…As it follows from the experimental research literature, GcMAF carries a free GalNAc moiety at the Thr 420 position of the amino acid sequence [6,11,[28][29][30][31]42,[44][45][46]. The highaffinity carbohydrate-binding receptors have been studied well [57].…”

Section: The Possible Mechanism Of Pm Activation By Gcmafmentioning

confidence: 99%

“…It is believed that the GcMAF precursor, vitamin D 3 -binding protein, is converted to the macrophage-activating factor via the partial deglycosylation of trisaccharide linked to Thr 420 by the O-glycosidic bond, giving rise to the terminal GalNAc residue. The available experimental data indicate that this very carbohydrate is responsible for the macrophage-activating ability of GcMAF [11,[27][28][29][30][31]. Some preparations are called GcMAF, but, in fact, are not GcMAF.…”

Section: Introductionmentioning

confidence: 99%

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The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF

Kirikovich,

Levites,

Proskurina

et al. 2023

IJMS

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Group-specific component macrophage-activating factor (GcMAF) is the vitamin D3-binding protein (DBP) deglycosylated at Thr420. The protein is believed to exhibit a wide range of therapeutic properties associated with the activation of macrophagal immunity. An original method for GcMAF production, DBP conversion to GcMAF, and the analysis of the activating potency of GcMAF was developed in this study. Data unveiling the molecular causes of macrophage activation were obtained. GcMAF was found to interact with three CLEC10A derivatives having molecular weights of 29 kDa, 63 kDa, and 65 kDa. GcMAF interacts with high-molecular-weight derivatives via Ca2+-dependent receptor engagement. Binding to the 65 kDa or 63 kDa derivative determines the pro- and anti-inflammatory direction of cytokine mRNA expression: 65 kDa—pro-inflammatory (TNF-α, IL-1β) and 63 kDa—anti-inflammatory (TGF-β, IL-10). No Ca2+ ions are required for the interaction with the canonical 29 kDa CLEC10A. Both forms, DBP protein and GcMAF, bind to the 29 kDa CLEC10A. This interaction is characterized by the stochastic mRNA synthesis of the analyzed cytokines. Ex vivo experiments have demonstrated that when there is an excess of GcMAF ligand, CLEC10A forms aggregate, and the mRNA synthesis of analyzed cytokines is inhibited. A schematic diagram of the presumable mechanism of interaction between the CLEC10A derivatives and GcMAF is provided. The principles and elements of standardizing the GcMAF preparation are elaborated.

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Section: The Possible Mechanism Of Pm Activation By Gcmafmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF

Kirikovich,

Levites,

Proskurina

et al. 2023

IJMS

View full text Add to dashboard Cite

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The association between vitamin D binding protein levels and periodontal status: A systematic review

Dhaif,

Garcia‐Sanchez,

Albuquerque

et al. 2024

J of Periodontal Research

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Background and ObjectivesVitamin D binding protein (DBP) is biosynthesised in the liver and is predominantly expressed in serum. Its primary role centres on facilitating the systemic transportation of vitamin D and its metabolites, notably 25‐hydroxyvitamin D, to specific target tissues where vitamin D exerts its biological functions. Due to the paucity of studies, it is unclear whether there is an association between DBP and periodontal status and thus its potential use as a diagnostic biomarker. Therefore, the aim of the systematic review is to investigate the association between DBP in periodontal disease.MethodsTwo independent reviewers (YD and RG) performed a systematic literature search of English publications using several databases including MEDLINE (OVID interface, 1946 onwards), EMBASE (OVID interface, 1974 onwards), and Global Health (OVID interface, 1973 onwards). This search strategy enabled the identification of relevant publications and the development of a comprehensive library of studies. Studies were included based on previously agreed eligibility criteria. Of the eight studies included as part of this systematic review, seven were case–control studies and one was a cross‐sectional study. The quality assessment was based on the Newcastle–Ottawa Scale (NOS) for case–control studies and the modified NOS for the cross‐sectional study.ResultsThe NOS quality assessment was ‘favorable’ for 6 included case control studies; and ‘fair’ for one study. The modified NOS quality assessment for the single cross‐sectional study demonstrated a medium risk of bias. The results of the majority of the included studies indicated a statistically significant higher concentration of DBP levels in individuals with periodontitis in comparison to those who were periodontally healthy. This trend held true irrespective of the sampling method employed for the assessment of DBP concentration.ConclusionThe results summarised in this systematic review indicate a positive association between DBP and periodontitis. Nonetheless, there is a need for longitudinal, prospective trials, to confirm the use of DBP as a potential biomarker for the diagnosis of periodontitis.

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Vitamin D and Vitamin D Binding Protein in Health and Disease 2.0

Abstract: Vitamin D, often referred to as the “sunshine nutrient”, has gained considerable attention in recent years due to its multifaceted impact on health and disease [...]

Cited by 2 publications

References 9 publications

The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF

The Molecular Aspects of Functional Activity of Macrophage-Activating Factor GcMAF

The association between vitamin D binding protein levels and periodontal status: A systematic review

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