Vitamin D and Calcium Supplementation Accelerate Vascular Calcification in a Model of Pseudoxanthoma Elasticum

Bouderlique, Elise; Tang, Ellie; Coudert, Amélie E.; Bazin, Dominique; Borondics, Ferenc; Haymann, Jean‐Philippe; Lefthériotis, Georges; Martin, Ludovic; Daudon, Michel; Letavernier, Emmanuel

doi:10.3390/ijms23042302

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…An increasing amount of evidence demonstrates that optical photothermal infrared (OPTIR) microspectroscopy is a valuable imaging and spectroscopy tool that can extract chemical information about biomolecules directly from individual cells and tissues at submicron resolution. − OPTIR is a novel infrared spectromicroscopy technique that exploits the photothermal effect . Specifically, infrared (IR) absorption induces a photothermal response consisting of thermal expansion and a change in the index of refraction of the sample, which leads to an intensity change of the probe light and allows the detection of the photothermal response.…”

Section: Introductionmentioning

confidence: 99%

Fluorescently Guided Optical Photothermal Infrared Microspectroscopy for Protein-Specific Bioimaging at Subcellular Level

Prater¹,

Bai

Konings

et al. 2023

J. Med. Chem.

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Infrared spectroscopic imaging is widely used for the visualization of biomolecule structures, and techniques such as optical photothermal infrared (OPTIR) microspectroscopy can achieve <500 nm spatial resolution. However, these approaches lack specificity for particular cell types and cell components and thus cannot be used as a stand-alone technique to assess their properties. Here, we have developed a novel tool, fluorescently guided optical photothermal infrared microspectroscopy, that simultaneously exploits epifluorescence imaging and OPTIR to perform fluorescently guided IR spectroscopic analysis. This novel approach exceeds the diffraction limit of infrared microscopy and allows structural analysis of specific proteins directly in tissue and single cells. Experiments described herein used epifluorescence to rapidly locate amyloid proteins in tissues or neuronal cultures, thus guiding OPTIR measurements to assess amyloid structures at the subcellular level. We believe that this new approach will be a valuable addition to infrared spectroscopy providing cellular specificity of measurements in complex systems for studies of structurally altered protein aggregates.

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Section: Introductionmentioning

confidence: 99%

Fluorescently Guided Optical Photothermal Infrared Microspectroscopy for Protein-Specific Bioimaging at Subcellular Level

Prater¹,

Bai

Konings

et al. 2023

J. Med. Chem.

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“…The results of a study by Young et al suggested that serum vitamin D deficiency may contribute to the progression of coronary calcification [ 28 ]. In addition, Bouderlique et al revealed that vascular calcification was accelerated in a murine model of pseudoxanthoma elasticum when vitamin D or calcium supplements were added [ 29 ]. When the serum vitamin D level is at the right concentration, it can inhibit the abovementioned process and slow the progression of AAC.…”

Section: Discussionmentioning

confidence: 99%

Association between Serum 25-Hydroxyvitamin D and Abdominal Aortic Calcification: A Large Cross-Sectional Study

Liu

Zuo

Wang

et al. 2023

International Journal of Clinical Practice

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In the American population, the relationship between the standardized serum 25-hydroxyvitamin D (25(OH)D) concentration and the risk of abdominal aortic calcification (AAC) is unclear. The purpose of our study was to investigate the relationship between serum 25(OH)D concentration and AAC risk. Participants from the National Health and Nutrition Examination Survey (NHANES) between 2013 and 2014 were analyzed cross sectionally. An analysis of the relationship between serum 25(OH)D concentration and incident AAC and severe AAC (SAAC) was based on the restricted cubic spline (RCS) and multivariable logistic regression model. In addition, generalized additive models with smooth functions were used to evaluate the relationship between serum 25(OH)D concentration and the degree of AAC. Finally, a subgroup analysis was conducted. There were a total of 3,040 individuals in our study. The serum 25(OH)D concentration was divided into quartiles (Q1: 9.37–50.5 nmol/L; Q2: 50.6–67.2 nmol/L; Q3: 67.3–85.8 nmol/L; and Q4: 85.9–318.0 nmol/L); the lowest quartile served as the reference group (Q1). After adjusting for known confounding variables, compared with the lowest quartile (Q1) of serum 25(OH)D concentration, the odds ratios with 95% confidence intervals for AAC and SAAC across the quartiles (Q2, Q3, and Q4) were (1.042 (0.812, 1.338), 0.863 (0.668, 1.115), and 1.022 (0.787, 1.327)) and (1.48 (0.87, 2.52), 1.70 (1.01, 2.92), and 2.13 (1.19, 3.86)), respectively. As shown by the RCS plot, the serum 25(OH)D concentration was associated with the risk of AAC/SAAC in a U-shaped pattern ( P for nonlinearity <0.05). In addition, the degree of AAC decreased at first and then increased as the serum 25(OH)D concentration increased. In conclusion, a U-shaped relationship existed between serum 25(OH)D concentration and the risk of AAC and SAAC. Consequently, the risk of AAC and SAAC may be mitigated with regular monitoring and vitamin D supplementation.

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“…We have already investigated biological samples from different organs and parts of the human body, namely kidney [177][178][179][180][181][182][183], prostate [184,185], breast [13,103], thyroid [104][105][106], cartilage [186][187][188][189][190][191][192][193], bone [194][195][196][197], tooth [198], pancreas [199], skin and hairs [53][54][55]147,165,168,[200][201][202][203] and cardiovascular system [140,204,205]. We have also investigated cells [206], mice [207][208][209][210], and medical devices [211,212], as well as chemical compounds identified in k...…”

Section: Characterization By Physicochemical Techniquesmentioning

confidence: 99%

Foreword to microcrystalline pathologies: combining clinical activity and fundamental research at the nanoscale

Bazin¹,

Daudon²,

Frochot³

et al. 2022

Comptes Rendus. Chimie

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This contribution underlines the key role of physicochemical characterisation techniques in the area of medical research. The starting point centres on the Mid-InfraRed platform located at the Tenon hospital and dedicated to multidisciplinary functional investigations. In the last two decades, we have enhanced this platform by creating a network combining researchers from varied disciplines such as physicists, chemists, and clinicians. The resultant research dynamism is underscored by metrics such as 71 references in Pubmed and 129 in Web of Science, and the high impact of the journals in which we have published (New England Journal of Medicine, Kidney International, Chemical Review. . . ). It is of paramount importance to disseminate these physicochemical techniques among young doctors, and to establish collaborations with appropriate private companies.

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Vitamin D and Calcium Supplementation Accelerate Vascular Calcification in a Model of Pseudoxanthoma Elasticum

Cited by 7 publications

References 28 publications

Fluorescently Guided Optical Photothermal Infrared Microspectroscopy for Protein-Specific Bioimaging at Subcellular Level

Fluorescently Guided Optical Photothermal Infrared Microspectroscopy for Protein-Specific Bioimaging at Subcellular Level

Association between Serum 25-Hydroxyvitamin D and Abdominal Aortic Calcification: A Large Cross-Sectional Study

Foreword to microcrystalline pathologies: combining clinical activity and fundamental research at the nanoscale

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