Vitamin content of purified typhus rickettsiae, tobacco mosaic virus, and coliphage T6

Kleinschmidt, W. J.; Holmes, D. H.; Behrens, Otto K.

doi:10.1016/0006-3002(56)90152-4

Cited by 7 publications

(7 citation statements)

References 18 publications

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“…The inhibition of microorganisms by salicylic and acetylsalicylic acids has been attributed to interference in a step in pantothenic acid metabolism (IvAnovics, 1942a, b;Maas, 1952). Our finding that pantothenic acid did not reverse acetylsalicylic acid inhibition was not surprising in view of the report by Kleinschmidt et al (1956) that purified rickettsiae do not contain significant amounts of pantothenic acid.…”

Section: ]supporting

confidence: 80%

“…This would indicate that p-aminobenzoic acid is not involved in folic acid metabolism of rickettsiae or that a folic acid different from pteroylglutamic acid or leucovorin is produced. The results of Kleinschmidt et al (1956) which indicate that rickettsiae do not contain significant amounts of folic acid tend to support the first explanation. Davis (1951b) and Davis and Maas (1952) furnished the most satisfactory bacterial model which can be used as the starting point for an explanation of competitive inhibition in rickettsiae.…”

Section: ]mentioning

confidence: 61%

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INHIBITION BY ACETYLSALICYLIC ACID OF RICKETTSIAL STRAINS RESISTANT TOp-AMINOBENZOIC ACID

1959

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Two p-aminobenzoic acid-resistant mutants of the Madrid E strain of Rickettsia prowazekii, pr and prr have been described in previous publications (Weiss et al., 1957; 1959). It has been shown that certain properties, such as antigenic specificity, infectivity, and virulence for the chick embryo and cotton rat, have not changed in these strains. However, when the study was extended to susceptibility to compounds chemically related to p-aminobenzoic acid, new characteristics were detected. These characteristics are described in this paper.' MATERIALS AND METHODS Rickettsiae. The Madrid E strain (Perez Ga-Ilardo and Fox, 1948) of R. prowazekii and its following substrains were used: psr, pr, and prr, which can be considered as first, second, and third-step mutants, respectively, to p-aminobenzoic acid resistance; Ar, resistant to acetylsalicylic acid; Qr, resistant to 2, 3-dimethylquinoxaline-1 ,4-dioxide; and pQr resistant to both p-aminobenzoic acid and the quinoxaline compound. The selection of these strains was described in previous publications (Weiss et al., 1957; 1959), except for psr and Ar which are described in later sections of this paper.2 Rickettsial pools were prepared from the infected yolk sacs of chick embryos and diluted in Bovarnick's isotonic solution (Bovarnick et al., 1950). Chemical compounds. The chemical compound(ls were obtained from several commercial sources. Solutions were prepared immediately before use, except for leucovorin and sulfadiazine which were received as solutions. The compounds were dissolved in distilled water with sufficient sodium 1 Some of this work was presented at the Seventh International Congress for Microbiology in Stockholm, Sweden, August 4 to 9, 1958. 2 The p-aminobenzoic acid-resistant strains were designated PABr, PABrr, and PABrrQr in a previous publication (Weiss et al., 1959).

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Section: ]supporting

confidence: 80%

Section: ]mentioning

confidence: 61%

INHIBITION BY ACETYLSALICYLIC ACID OF RICKETTSIAL STRAINS RESISTANT TOp-AMINOBENZOIC ACID

1959

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“…The demonstration of folic acid in rickettsiae (Kleinschmidt, Holmes, and Behrens, 1956;Myers, 1958) and in the psittacosis group (Colon and Moulder, 1958;Colon, 1961) is suggestive of the presence of folic acid-mediated reactions in these organisms. The data presented in this paper support this view, at least for C. burnetii.…”

Section: Discussionmentioning

confidence: 99%

SYNTHESIS OF SERINE BY COXIELLA BURNETII

Myers

Paretsky

1961

J Bacteriol

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AND D. PARETSKY. Synthesis of serine by Coxiella burnetii. J. Bacteriol. 82:761-763. 1961.-Enzyme preparations from suspensions of purified Coxiella burnetii are capable of synthesizing serine from formaldehyde and glycine-2-C'4 in the presence of tetrahydrofolic acid. Evidence for the reaction is obtained by isolating and identifying radioactive serine and serine derivatives. The data suggest a role for folic acid in these rickettsiae, and extend the knowledge of hostindependent biosynthetic competencies of these highly parasitic microorganisms.

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“…The biochemical functions of the folic acid series have been well reviewed (Huennekens and Osborn, 1959; Rabinowitz, 1960), but there is little information on the significance of the presence and function of folates in parasitic organisms. Folic acid was demonstrated in the rickettsiae Rickettsia mooseri (Kleinschmidt, Holmes, and Behrens, 1956) and Coxiella burnetii (Myers, 1958), in the malarial parasite Plasmodium lophurae (Trager, 1959), and in the psittacosis group of viruses (Col6n and Moulder, 1958;Col6n, 1960Col6n, , 1962. Dewey and Kidder 1 National Defense Education Act Fellow.…”

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confidence: 99%

Studies on the Physiology of Rickettsiae Iv

1963

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, M. SILVERMAN, AND D. PARETSKY. Studies on the physiology of rickettsiae. IV. Folic acids of Coxiella burnetii. J. Bacteriol. 85:37-41. 1963.-Yolk, yolk sac, and embryo tissues of uninfected eggs, and those infected with Coxiella burnetii, were analyzed for folic acid derivatives by employing diethylaminoethyl (DEAE)-cellulose column chromatography. Infected tissues contained quantitatively less folate, but the elution profiles of both infected and uninfected tissues were identical. Purified C. burnetii contained some types of folate apparently unique to these rickettsiae, and not found in infected tissue. The major folate fraction of C. burnetii was partially characterized by (i) elution position from DEAE columns; (ii) treatment with conjugase; (iii) growth response by Lactobacillus casei, Streptococcus faecalis R, and Pediococcus cerevisiae; and (iv) response to oxidation, reduction, and formylation. Folic acid or its derivatives are widely distributed among multicellular and unicellular forms, in free-living as well as in parasitic micro

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Vitamin content of purified typhus rickettsiae, tobacco mosaic virus, and coliphage T6

Cited by 7 publications

References 18 publications

INHIBITION BY ACETYLSALICYLIC ACID OF RICKETTSIAL STRAINS RESISTANT TOp-AMINOBENZOIC ACID

INHIBITION BY ACETYLSALICYLIC ACID OF RICKETTSIAL STRAINS RESISTANT TOp-AMINOBENZOIC ACID

SYNTHESIS OF SERINE BY COXIELLA BURNETII

Studies on the Physiology of Rickettsiae Iv

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