Vitamin C Pretreatment Enhances the Antibacterial Effect of Cold Atmospheric Plasma

Helgadottir, Saga; Pandit, Santosh; Mokkapati, Venkata Raghavendra Subrahmanya Sar; Westerlund, Fredrik; Apell, S. Peter; Mijaković, Ivan

doi:10.3389/fcimb.2017.00043

Cited by 46 publications

(40 citation statements)

References 35 publications

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“…These findings are summarized in Figure 2. Our results [27] suggest that vitamin C can be used as an effective pre-treatment, to render CAP treatments more effective. This biofilmdestabilizing effects probably also accounts for previously reported effect of vitamin C in enhancing common antibiotic treatments, even against bacteria which are not directly killed by vitamin C [25,26].…”

Section: Combining Vitamin C With Cold Atmospheric Plasmamentioning

confidence: 56%

“…Since CAP treatments are inhibited by the biofilm matrix [12], we have examined whether vitamin C, which inhibits EPS production, could be used to enhance the effectiveness of CAP treatments. We found that the vitamin C pre-treatment made bacterial biofilms of Bacillus subtilis, Escherichia coli, P. aeruginosa and Staphylococcus epidermidis significantly more prone to extermination by CAP treatments [27]. These findings are summarized in Figure 2.…”

Section: Combining Vitamin C With Cold Atmospheric Plasmamentioning

confidence: 68%

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Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Pandit¹,

Mokkapati²,

Helgadottir³

et al. 2017

Clin Microbiol

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Cold atmospheric plasma (CAP) is increasingly used in medical applications for eradication of bacterial and tumor cells. CAP treatment devices, known as plasma jet pens, produce reactive oxygen and nitrogen species at atmospheric pressure and room temperature. The produced reactive species are concentrated in a small and precisely defined area, allowing for high precision medical treatments. CAP has been demonstrated as very effective against planktonic bacterial cells. Unfortunately, bacterial cells in biofilms are typically aggregated and protected by dense exopolymeric matrix, synthesized and secreted by the bacterial community. The main limitation in using CAP against bacterial biofilms is the thick protective matrix of extracellular polymers that shields bacterial cells within this complex architecture. CAP has also been shown to effectively eradicate tumor cells, but the main current limitation is the susceptibility of the surrounding healthy tissues to higher doses. We have recently demonstrated that vitamin C, a natural food supplement, can be used to destabilize bacterial biofilms and render them more susceptible to the CAP killing treatment. Here we discuss the possible impact that a pre-treatment with vitamin C could have on CAP applications in medicine. Specifically, we argue that vitamin C could enhance the effectiveness of CAP treatments against both the bacterial biofilms and some selected tumors.

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Section: Combining Vitamin C With Cold Atmospheric Plasmamentioning

confidence: 56%

Section: Combining Vitamin C With Cold Atmospheric Plasmamentioning

confidence: 68%

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Pandit¹,

Mokkapati²,

Helgadottir³

et al. 2017

Clin Microbiol

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“…For using chemical methods to enhance plasma efficiency, modified atmosphere is one of the most studied successful methods (Misra, Moiseev et al., ), whereas both food‐based sanitizers and natural antimicrobials (for example, lemongrass oil, and vitamin C) are alternative approaches (Cui, Wu, Li, & Lin, ; Helgadottir et al., ). Matan, Puangjinda, Phothisuwan, and Nisoa () observed the synergistic antibacterial activity of green tea extract and atmospheric RF plasma against pathogens on fresh‐cut dragon fruit, and results indicated that 5.0% of green tea pretreatment for 60 s followed by plasma exposure for 60 s at 40 W extended the shelf life of fresh‐cut dragon fruit to at least 15 days at 4 °C.…”

Section: Fundamentals Of Nonthermal or Cold Plasmamentioning

confidence: 99%

Cold Plasma‐Mediated Treatments for Shelf Life Extension of Fresh Produce: A Review of Recent Research Developments

Pan

Cheng

Sun

2019

Comp Rev Food Sci Food Safe

148

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Fresh produce, like fruits and vegetables, are important sources of nutrients and health‐promoting compounds. However, incidences of foodborne outbreaks associated with fresh produce often occur; it is thus important to develop and expand decay‐control technologies that can not only maintain the quality but can also control the biological hazards in postharvest, processing, and storage to extend their shelf life. It is under such a situation that plasma‐mediated treatments have been developed as a novel nonthermal processing tool, offering many advantages and attracting much interest from researchers and the food industry. This review summarizes recent developments of cold plasma technology and associated activated water for shelf life extension of fresh produce. An overview of plasma generation and its physical–chemical properties as well as methods for improving plasma efficiency are first presented. Details of using the technology as a nonthermal agent in inhibiting spoilage and pathogenic microorganisms, inactivating enzymes, and modifying the barrier properties or imparting specific functionalities of packaging materials to extend shelf life of food produce are then reviewed, and the effects of cold plasma‐mediated treatment on microstructure and quality attributes of fresh produce are discussed. Future prospects and research gaps of cold plasma are finally elucidated. The review shows that atmospheric plasma‐mediated treatments in various gas mixtures can significantly inhibit microorganisms, inactive enzyme, and modify packaging materials, leading to shelf life extension of fresh produce. The quality attributes of treated produce are not compromised but improved. Therefore, plasma‐mediated treatment has great potential and values for its application in the food industry.

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“…have been reported by El-Gebaly et al [31]. On the contrary, other studies [30,[32][33][34] have found that ascorbic acid did not inhibit the growth of E. coli and P. aeruginosa but made them more resistant to antimicrobials and physical disinfection.…”

Section: Discussionmentioning

confidence: 88%

“…In turn, Mirani et al [26] have observed that ascorbic acid inhibited EPS production and staphylococcal biofilm formation but the survived bacteria tolerated its toxic concentration. Helgadóttir et al [30] have noticed the reduction of 89.9% of S. epidermidis, E. coli, and P. aeruginosa bacteria number on catheters after vitamin C treatment. Similar results against mature biofilms of uropathogenic bacteria (E. coli, Klebsiella spp., Citrobacter spp., Enterobacter spp., Proteus spp., and Pseudomonas spp.)…”

Section: Discussionmentioning

confidence: 97%

Ascorbic Acid Changes Growth of Food-Borne Pathogens in the Early Stage of Biofilm Formation

et al. 2020

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Since bacterial biofilm may contribute to the secondary contamination of food during the manufacturing/processing stage there is a need for new methods allowing its effective eradication. Application of food additives such as vitamin C already used in food industry as antioxidant food industry antioxidants may be a promising solution. The aim of this research was evaluation of the impact of vitamin C (ascorbic acid), in a range of concentrations 2.50 µg mL−1–25.0 mg mL−1, on biofilms of Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes strains isolated from food. The efficacy of ascorbic acid was assessed based on the reduction of optical density (λ = 595 nm). The greatest elimination of the biofilm was achieved at the concentration of vitamin C of 25.0 mg mL−1. The effect of the vitamin C on biofilm, however, was strain dependent. The concentration of 25.0 mg mL−1 reduced 93.4%, 74.9%, and 40.5% of E. coli, L. monocytogenes, and S. aureus number, respectively. For E. coli and S. aureus lower concentrations were ineffective. In turn, for L. monocytogenes the biofilm inhibition was observed even at the concentration of 0.25 mg mL−1. The addition of vitamin C may be helpful in the elimination of bacterial biofilms. Nonetheless, some concentrations can induce growth of the pathogens, posing risk for the consumers’ health.

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Vitamin C Pretreatment Enhances the Antibacterial Effect of Cold Atmospheric Plasma

Cited by 46 publications

References 35 publications

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Combination of Cold Atmospheric Plasma and Vitamin C Effectively Disrupts Bacterial Biofilms

Cold Plasma‐Mediated Treatments for Shelf Life Extension of Fresh Produce: A Review of Recent Research Developments

Ascorbic Acid Changes Growth of Food-Borne Pathogens in the Early Stage of Biofilm Formation

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