Vitamin C or Vitamin B6 supplementation prevent the oxidative stress and decrease of prostacyclin generation in homocysteinemic rats

Mahfouz, Mahmoud; Kummerow, F.A.

doi:10.1016/j.biocel.2004.01.028

Cited by 55 publications

(40 citation statements)

References 73 publications

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“…This enzyme synthesizes pyridoxine, the precursor for pyridoxal-5-P, an important cofactor for many enzymatic reactions including ATs and DECARBs (see enzyme class cofactors, Supplemental Table S3). Vitamin B 6 has also been shown to have antioxidant activity (Mahfouz and Kummerow, 2004). ALDH, elevated 2.4-fold in high salinity and classified under energy production and conversion, could also be considered an antioxidant.…”

Section: General Stress Response Proteinsmentioning

confidence: 99%

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

et al. 2011

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Dimethylsulfoniopropionate (DMSP) plays important roles in oceanic carbon and sulfur cycling and may significantly impact climate. It is a biomolecule synthesized from the methionine (Met) pathway and proposed to serve various physiological functions to aid in environmental stress adaptation through its compatible solute, cryoprotectant, and antioxidant properties. Yet, the enzymes and mechanisms regulating DMSP production are poorly understood. This study utilized a proteomics approach to investigate protein changes associated with salinity-induced DMSP increases in the model sea-ice diatom Fragilariopsis cylindrus (CCMP 1102). We hypothesized proteins associated with the Met-DMSP biosynthesis pathway would increase in relative abundance when challenged with elevated salinity. To test this hypothesis axenic log-phase cultures initially grown at a salinity of 35 were gradually shifted to a final salinity of 70 over a 24-h period. Intracellular DMSP was measured and two-dimensional gel electrophoresis was used to identify protein changes at 48 h after the shift. Intracellular DMSP increased by approximately 85% in the hypersaline cultures. One-third of the proteins increased under high salinity were associated with amino acid pathways. Three protein isoforms of S-adenosylhomo-cysteine hydrolase, which synthesizes a Met precursor, increased 1.8-to 2.1-fold, two isoforms of S-adenosyl Met synthetase increased 1.9-to 2.5-fold, and S-adenosyl Met methyltransferase increased by 2.8-fold, suggesting active methyl cycle proteins are recruited in the synthesis of DMSP. Proteins from the four enzyme classes of the proposed algal Met transaminase DMSP pathway were among the elevated proteins, supporting our hypothesis and providing candidate genes for future characterization studies.

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Section: General Stress Response Proteinsmentioning

confidence: 99%

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

et al. 2011

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“…Furthermore, results from these studies have shown that CRP and pro-inflammatory IL levels are unchanged after vitamin supplementation (57,58) . Antioxidant activity of vitamin B 6 supplementation has been observed in a study performed on rats, but the exact mechanism is unclear (59) . Several large, prospective trials have been conducted in recent years with the principal aim of studying the effects of lowering serum homocysteine concentrations with the use of B vitamins, including vitamin B 6 , on cardiovascular events (60,61) .…”

Section: Vitamin B 6 Supplementation Inflammation and Cvd Preventionmentioning

confidence: 99%

Vitamin B₆: a challenging link between nutrition and inflammation in CVD

Lotto

Friso

2011

Br J Nutr

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The objective of the present review is to highlight the relationship between low vitamin B 6 status and CVD through its link with inflammation. While overt vitamin B 6 deficiency is uncommon in clinical practice, increasing evidence suggests that marginal vitamin B 6 deficiency is rather frequent in a consistent proportion of the population and is related to an increased risk of inflammation-related diseases. Ample evidence substantiates the theory of atherosclerosis as an inflammatory disease, and low plasma vitamin B 6 concentrations have been related to increased CVD risk. Several studies have also shown that low vitamin B 6 status is associated with rheumatoid arthritis and chronic inflammatory bowel diseases, both of which hold an underlying chronic inflammatory condition. Furthermore, the inverse association observed between inflammation markers and vitamin B 6 supports the notion that inflammation may represent the common link between low vitamin B 6 status and CVD risk. In addition to the epidemiological evidence, there are a number of cell culture and animal studies that have suggested several possible mechanisms relating impaired vitamin B 6 status with chronic inflammation. A mild vitamin B 6 deficiency characterises, in most cases, a subclinical at-risk condition in inflammatory-linked diseases which should be addressed by an appropriate individually tailored nutritional preventive or therapeutic strategy.

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“…After the operation, rats were allowed to recover and have free access to a standard laboratory diet and tap water. L-arginine was given in a dose of 1.25 g/L in the drinking water (approximately 0.2g/kg/day), [32] and vitamins E and C and catechin were mixed with food (400 mg/kg food, 500 mg/kg food, 100 mg/kg/day, respectively, [33][34][35][36] and L-carnitine 500 mg/kg intraperitoneally). [37] Treatment started one week after RMR and continued for 12 weeks.…”

Section: Animal Grouping and Treatment Regimenmentioning

confidence: 99%

Multiple Antioxidants and L-Arginine Modulate Inflammation and Dyslipidemia in Chronic Renal Failure Rats

Korish

2010

Renal Failure

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The kidney is an important source of L-arginine, the endogenous precursor of nitric oxide (NO). Surgical problems requiring extensive renal mass reduction (RMR) decrease renal NO production, leading to multiple hemodynamic and homeostatic disorders manifested by hypertension, oxidative stress, and increased inflammatory cytokines. Using the RMR model of chronic renal failure (CRF), we assessed the effects of twelve weeks' administration of L-arginine and/or a mixture of antioxidants (L-carnitine, catechin, vitamins E and C) on plasma cytokines, soluble intercellular adhesion molecule-1 (sICAM-1), nitrate and nitrites (NO 2 /NO 3 ), lipid profile, blood pressure, and renal function. CRF rats showed increased plasma IL-1a, IL1-b, IL-6, TNF-a, and sICAM-1 levels and decreased anti-inflammatory cytokines IL-4 and 10 levels, hypertension, and dyslipidemia. L-arginine treatment improved kidney functions, decreased systolic blood pressure, and decreased inflammatory cytokines levels. Antioxidants administration decreased inflammatory cytokines and sICAM-1 levels and increased IL-4 levels. Combined use of both L-arginine and the antioxidant mixture were very effective in their tendency to recover normal values of kidney functions, plasma cytokines, sICAM-1, blood pressure, NO 2 /NO 3 , cholesterol, and triglycerides concentrations. Indeed, the effects of L-arginine and the antioxidants on the reduction of proinflammatory cytokines may open new perspectives in the treatment of uremia.

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Vitamin C or Vitamin B6 supplementation prevent the oxidative stress and decrease of prostacyclin generation in homocysteinemic rats

Cited by 55 publications

References 73 publications

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

Vitamin B₆: a challenging link between nutrition and inflammation in CVD

Multiple Antioxidants and L-Arginine Modulate Inflammation and Dyslipidemia in Chronic Renal Failure Rats

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Vitamin C or Vitamin B6 supplementation prevent the oxidative stress and decrease of prostacyclin generation in homocysteinemic rats

Cited by 55 publications

References 73 publications

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

Proteomic Analysis of a Sea-Ice Diatom: Salinity Acclimation Provides New Insight into the Dimethylsulfoniopropionate Production Pathway

Vitamin B6: a challenging link between nutrition and inflammation in CVD

Multiple Antioxidants and L-Arginine Modulate Inflammation and Dyslipidemia in Chronic Renal Failure Rats

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Vitamin B₆: a challenging link between nutrition and inflammation in CVD