Vitamin C, flower color and ploidy variation of hybrids from a ploidy-unbalanced Actinidia interspecific cross and SSR characterization

Zhang, Lei; Li, Zuozhou; Wang, Yan-Chang; Jiang, Zhengwang; Wang, Shengmei; Huang, Hongwen

doi:10.1007/s10681-010-0194-z

Cited by 27 publications

(20 citation statements)

References 60 publications

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“…This genus is considered native to China, since most of the species occur in the southwest of the country. Over the past three decades, kiwifruit has become an economically important fruit crop due to its nutritional quality and unique flavor, and its cultivation worldwide has substantially increased (Ferguson and Huang, 2007; Zhang L. et al, 2010). The annual production of kiwifruit was 3.26 million metric tons in 2013 (http://faostat3.fao.org/).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

et al. 2016

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Genomic and transcriptomic data on kiwifruit (Actinidia chinensis) in public databases are very limited despite its nutritional and economic value. Previously, we have constructed and sequenced nine fruit RNA-Seq libraries of A. chinensis “Hongyang” at immature, mature, and postharvest ripening stages of fruit development, and generated over 66.2 million paired-end and 24.4 million single-end reads. From this dataset, here we have identified 7051 long noncoding RNAs (lncRNAs), 29,327 alternative splicing (AS) events and 2980 novel protein-coding genes that were not annotated in the draft genome of “Hongyang.” AS events were demonstrated in genes involved in the synthesis of nutritional metabolites in fruit, such as ascorbic acids, carotenoids, anthocyanins, and chlorophylls, and also in genes in the ethylene signaling pathway, which plays an indispensable role in fruit ripening. Additionally, transcriptome profiles and the contents of sugars, organic and main amino acids were compared between immature, mature, and postharvest ripening stages in kiwifruits. A total of 5931 differentially expressed genes were identified, including those associated with the metabolism of sugar, organic acid, and main amino acids. The data generated in this study provide a foundation for further studies of fruit development and ripening in kiwifruit, and identify candidate genes and regulatory elements that could serve as targets for improving important agronomic traits through marker assisted breeding and biotechnology.

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Section: Introductionmentioning

confidence: 99%

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

et al. 2016

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“…SSR markers have been widely applied to construct linkage maps of the rice (McCouch et al 2002) and tomato (Frary et al 2005) genomes. Such markers can also be used to assist in selection in breeding and backcrossing for a specific trait or quantitative traits, in genotyping species (He et al 2003;Gong and Geng 2010;Zorrilla-Fontanesi et al 2011), and in cultivars identification (Tantasawat et al 2010;Zhang et al 2010) and serve as effective tools in plant germplasm diversity analysis (Guilford et al 1997;Gomez et al 2008;Mujaju et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Development of novel microsatellite markers for effective applications in Anthurium cultivar identification

JauYueh

Chuang

2012

Euphytica

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Anthurium andraeanum is one of the most economically important floral crops and potted flowers marketed worldwide. Microsatellite markers are currently the preferred molecular marker owing to the many desirable attributes, including hypervariability, codominance, and amenability to high-throughput genotyping; however, there are few polymorphic molecular markers available for Anthurium. The object of this study was to develop and characterize novel microsatellite markers using the Araceae sequences in GenBank of the National Center for Biotechnology Information (NCBI) to contribute to molecular identification for cultivar protection. Using 1,579 Araceae expressed sequence tags (ESTs) and the related nucleotide sequences, 100 candidates contained simple sequence repeat (SSR) motifs that were suitable for primer design. Furthermore, 100 pairs of SSR primers were screened against a set of 28 diverse genotypes representing 24 cultivars that included four registration cultivars which were bred from the Taiwan Agricultural Research Institute (TARI) and 20 commercial cultivars, appended with three hybrid progeny and a mutant line. From the selected six polymorphic SSR loci, 52 alleles were amplified and 27 distinct genotypes were found, except for 'Tropical' and its mutant, with a mean number of eight alleles per locus. The polymorphism information content (PIC) ranged from 0.86 to 0.93. Based on these results, we proposed a key identification set using four microsatellite markers that is sufficient to discriminate among 24 cultivars. Because the Anthurium microsatellite markers developed in this study are primarily from expressed sequence tags or related genomic sequences, they can be used for cultivar identification and, accordingly, contribute to genetic evaluations in breeding programs.

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“…(2) the selections (e.g., diploid 'Lvzhu,' 'Mantianhong') that originated from interspecific crosses resulting in novel fruit traits (Beatson et al 2007) have high genetic diversity because of hybridization (Zhang et al 2010); and (3) the hybrids (e.g., Jinkui M2) generated by interploid crosses can be productive with improved genetic variation by breaking the interploid fertility block (Yan et al 1997;Seal et al 2012) and altering the (1) Fig. 2).…”

Section: Cultivar Diversity and Genetic Variabilitymentioning

confidence: 99%

Genetic diversity in kiwifruit polyploid complexes: insights into cultivar evaluation, conservation, and utilization

Liu

et al. 2014

Tree Genetics & Genomes

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Understanding the extent and partitioning of crop genetic diversity is necessary for conserving and utilizing their genetic potentials for breeding. In the present study, fluorescence-labeled amplified fragment length polymorphism markers were used to characterize the genetic diversity and relationships of 79 cultivars and also of 122 F1 hybrids which resulted from six kiwifruit interploid crosses. A high level of mean genetic diversity was detected (Hj > 0.22) for all cultivars investigated, without significant differences among diploids (2x), tetraploids (4x), and hexaploids (6x). This suggested that no significant genetic erosion occurred in these cultivars, which were directly selected from natural resources or created from crosses. The Unweighted Pair Group Method with Arithmetic Mean analysis of the genetic dissimilarity between cultivars showed three main groups mostly based on their three ploidy levels. Among these, the red-fleshed cultivars which were originally derived from 'Hongyang' clustered into one subgroup of group I, suggesting their unique genetic background despite they were marked as different cultivars used in the current kiwifruit industry. By analyzing the genetic variation of hybrids with variable ploidy levels, our genetic analyses further revealed that interploid crosses can increase the genetic diversity of F1 offsprings, especially from the parental combinations of 6x-2x and 6x-4x, in which both parents showed significant differences in morphology and genetic backgrounds. Based on these findings, strategies were proposed for the conservation and utilization of the current kiwifruit genetic resources for future breeding programs.

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Vitamin C, flower color and ploidy variation of hybrids from a ploidy-unbalanced Actinidia interspecific cross and SSR characterization

Cited by 27 publications

References 60 publications

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

Comprehensive Transcriptome Profiling Reveals Long Noncoding RNA Expression and Alternative Splicing Regulation during Fruit Development and Ripening in Kiwifruit (Actinidia chinensis)

Development of novel microsatellite markers for effective applications in Anthurium cultivar identification

Genetic diversity in kiwifruit polyploid complexes: insights into cultivar evaluation, conservation, and utilization

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