Vitamin C Enhances the <i>In vitro</i> Development of Porcine Pre‐implantation Embryos by Reducing Oxidative Stress

Hu, Jianhong; Cheng, De; Gao, Xing; Bao, Jian Chang; Ma, Xiaoling; Wang, H

doi:10.1111/j.1439-0531.2011.01982.x

Cited by 59 publications

(45 citation statements)

References 21 publications

(45 reference statements)

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“…10 Vitamin C is also an important cofactor in several enzymatic reactions such as histone demethylases and prolylhydroxylases. 11 Recent studies have demonstrated that vitamin C supplementation produces important effects on stem cell generation, proliferation and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Zhang

et al. 2016

Organogenesis

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ABSTRACT. Although adipose-derived stem cells (ADSCs) have demonstrated a promising potential for the applications of cell-based therapy and regenerative medicine, excessive reactive oxygen species (ROS) are harmful to ADSCs cell survival and proliferation. Vitamin C is an important antioxidant, and is often added into culture media as an essential micronutrient. However, its roles on the proliferation of human ADSCs have not been studied. Therefore, in this study, human ADSCs were isolated, and detected by flow cytometry for the analysis of their cell surface antigens. Cell proliferation and cell cycle progression were measured with cell counting kit-8 assay and flow cytometry, respectively. Western blotting was used to detect the expression levels of cyclin E1, p53, p21, and CDK2 proteins. The effect of vitamin C pretreatment on the production of hydrogen peroxide (H 2 O 2 )-mediated ROS in the ADSCs was evaluated by flow cytometry. Our results indicated that vitamin C treatment significantly increased cell proliferation, and changed the cell cycle distribution of ADSCs by decreasing the percentage of G 1 phase, and concurrently increased the percentage of S and G 2 /M phase. Western blot analysis indicated that vitamin C treatment up-regulated the expression levels of cyclin E1 and CDK2, but down-regulated p53 and p21 proteins expression, which contributed to cell proliferation and cell cycle progression. Vitamin C pretreatment significantly reduced the production of H 2 O 2 -induced ROS in the ADSCs. These findings suggest that vitamin C can promote the proliferation and cell cycle progression in the ADSCs possibly through regulation of p53-p21 signal pathway.

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Section: Introductionmentioning

confidence: 99%

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Zhang

et al. 2016

Organogenesis

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“…For instance, VitC is a well-known antioxidant and it has been reported that embryos exposed to VitC show reduced levels of reactive oxygen species (Wang et al 2002, Hu et al 2012, Li et al 2014) and increased reduced glutathione content (Wongsrikeao et al 2007, Zhang et al 2010, Mallol et al 2015, which lead to lower levels of apoptosis (Jeong et al 2006, Hu et al 2012, Li et al 2014, parameters which have been related to reduced speed of embryo development (Favetta et al 2004, Wale & Gardner 2010. Thus, the antioxidant effect of VitC could reduce DNA damage and checkpoint arrest, accelerating cell cycle progression in SCNT embryos.…”

Section: Discussionmentioning

confidence: 99%

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

et al. 2016

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Time-lapse monitoring of somatic cell nuclear transfer (SCNT) embryos may help to predict developmental success and increase birth and embryonic stem cells (ESC) derivation rates. Here, the development of ICSI fertilized embryos and of SCNT embryos, non-treated or treated with either psammaplin A (PsA) or vitamin C (VitC), was monitored, and the ESC derivation rates from the resulting blastocysts were determined. Blastocyst rates were similar among PsA-treated and VitC-treated SCNT embryos and ICSI embryos, but lower for nontreated SCNT embryos. ESC derivation rates were higher in treated SCNT embryos than in non-treated or ICSI embryos. Time-lapse microscopy analysis showed that non-treated SCNT embryos had a delayed development from the second division until compaction, lower number of blastomeres at compaction and longer compaction and cavitation durations compared with ICSI ones. Treatment of SCNT embryos with PsA further increased this delay whereas treatment with VitC slightly reduced it, suggesting that both treatments act through different mechanisms, not necessarily related to their epigenetic effects. Despite these differences, the time of completion of the third division, alone or combined with the duration of compaction and/or the presence of fragmentation, had a strong predictive value for blastocyst formation in all groups. In contrast, we failed to predict ESC derivation success from embryo morphokinetics. Time-lapse technology allows the selection of SCNT embryos with higher developmental potential and could help to increase cloning outcomes. Nonetheless, further studies are needed to find reliable markers for full-term development and ESC derivation success.

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“…Wang et al (2014) showed that supplementing culture with the proper concentration of vitamin C can protect cells against the damaging effects of ROS by suppressing ROS production and promote stem cell proliferation, regulating the expression of apoptosis-related genes. In fact, vitamin C inhibits pro-apoptotic signals and activates anti-apoptotic signals in vitro (Hu et al, 2012). On the other hand, vitamin E, which is known as a natural antioxidant, reacts with soluble free radicals in lipid membranes and prevents the process of lipid peroxidation by scavenging reactive oxygen species before they can cause damage to the cells (Moreira et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…This reduction in the survival rate of SSCs may be caused by the accumulation of toxic products of metabolism and ROS formation (Aliakbari et al, 2017). High levels of ROS production can increase the expression level of ROS-related genes such as bax and the proportion of the pro-apoptotic to anti-apoptotic genes markedly determines cell fate (Hu et al, 2012). In cells that are subject to apoptosis, bax, as a pro-apoptotic protein, forms pores on the mitochondrial membrane and expels cytochrome C from mitochondria to trigger the formation of a large protein complex called apoptosome.…”

Section: Discussionmentioning

confidence: 99%

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Optimization of <i>in vitro</i> culture and transfection condition of bovine primary spermatogonial stem cells

Jafarnejad¹,

Aminafshar²,

Zandi³

et al. 2018

SA J. An. Sci.

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The present study aimed to optimize the in vitro culture and transfection efficiency of bovine primary spermatogonial stem cells (SSCs). To this end, SSCs were obtained from newborn Holstein bull calves by two-step enzymatic digestion. After enrichment and culture, SSCs were characterized by using alkaline phosphatase (AP) staining and expression of vasa and thy1 genes as specific bovine SSC markers. To evaluate the effect of antioxidants on vitality, colony formation, and the expression of pro-and anti-apoptotic genes of bovine SSCs, various concentrations of vitamin C (5, 10, 25 and 50 µg/mL) and Trolox (a water soluble α-tocopherol analogue) (12.5, 25, 50 and 100 µg/mL) were added to the SSC culture medium. The results showed that SSCs treated with 50 µg/mL of vitamin C or 25 µg/mL of Trolox individually could increase cell viability and colony formation significantly in comparison with other concentrations and the control group. Additionally, the expressions of bax (as a pro-apoptotic gene) and bcl2 (as an anti-apoptotic gene) were significantly lower and higher than the control group, respectively. To optimize the transfection condition, the effective dosages of vitamin C or Trolox, with various concentrations of two transfection reagents (X-tremeGENE HP and Turbofect) and DNA, at day 8 of culture, were studied. Results showed that 1 μl X-tremeGENE HP or 0.5 μl Turbofect and 2 μg of DNA are the best concentrations for transfecting SSCs. However, X-tremeGENE HP expressed more potential for transfecting SSCs in comparison with Turbofect. Besides, no difference was observed between the use of defined doses of vitamin C or Trolox. ____________________________________________________________________________________

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Vitamin C Enhances the In vitro Development of Porcine Pre‐implantation Embryos by Reducing Oxidative Stress

Cited by 59 publications

References 21 publications

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction

Optimization of <i>in vitro</i> culture and transfection condition of bovine primary spermatogonial stem cells

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