Vitamin C attenuates detrimental effects of diabetes mellitus on sperm parameters, chromatin quality and rate of apoptosis in mice

Talebi, Ali Reza; Mangoli, Esmat; Nahangi, Hossein; Anvari, Morteza; Pourentezari, Majid; Halvaei, Iman

doi:10.1016/j.ejogrb.2014.07.007

Cited by 38 publications

(44 citation statements)

References 21 publications

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“…There are increasing bodies of evidence that show hypothyroidism is associated with the high production of ROS in testis. It is well known that high amounts of ROS negatively affect the quality of sperm DNA as well as sperm morphology and motility [11,22,31,32,38]. This study was conducted to determine the effect of hypothyroidism on the alteration of sperm chromatin, sperm morphology, and CatSper 1 and CatSper 2 genes expression.…”

Section: Introductionmentioning

confidence: 99%

Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice

Alipour

Jalali

Nikravesh

et al. 2018

Acta Biologica Hungarica

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There is an evident relationship between the fertilizing capacity of sperm and the normal morphology, quality chromatin, and motility of sperm. It is well known that thyroid hormones are the important regulators of testicular function. A correlation was found between the hypothyroidism and sperm damages. The present study was conducted to investigate the effects of hypothyroidism on sperm morphology, chromatin quality, and motility. For this purpose, 20 male mice were divided into the control and the hypothyroid groups that received 0.05% 6-n-propyl-2-thiouracil (PTU) for 35 days. Sperm morphology with Papanicolaou staining and sperm chromatin quality with both Aniline Blue (AB) and Toluidine blue (TB) staining were assessed. Besides, immunohistochemistry and real-time PCR were performed to evaluate the changes of cation sperm channel (CatSper) genes. A significant increase in the sperm chromatin condensation was found in the hypothyroid mice compared to the control mice (p < 0.05). Furthermore, a significant decrease was observed in the morphology of normal sperm in hypothyroid mice compared to the controls (p < 0.05). The results showed that Hypothyroidism could downregulate the expression of CatSper genes. Immunohistochemical data confirmed the real time-PCR results. Furthermore, the results showed that hypothyroidism could adversely affect sperm morphology, sperm chromatin condensation, and CatSper gene expression in mice and these abnormalities may be related to the excessive production of reactive oxygen species (ROS) in a hypothyroid state.

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Section: Introductionmentioning

confidence: 99%

Assessment of sperm morphology, chromatin integrity, and catSper genes expression in hypothyroid mice

Alipour

Jalali

Nikravesh

et al. 2018

Acta Biologica Hungarica

View full text Add to dashboard Cite

show abstract

“…[3][4][5][6][7][8] Values are expressed as mean±SD (n=3). DM impairs sperm parameters and decreases the serum testosterone levels.…”

Section: Effect Of Pgps On Semen Quality Parameters In Kkay Micementioning

confidence: 99%

“…DM impairs sperm parameters and decreases the serum testosterone levels. [3][4][5][6][7][8] Intragastric treatment with PGPs significantly improved the density, movement, and rate of normal morphology of sperm of KKAy mice compared with those in the control group (P<.05; Table 3). Previous studies reported that other polysaccharides protect the reproductive function of the male animal and prevent spermatogenic cell apoptosis; these polysaccharides include those derived from Lycium barbarum, 15,16 Portulace oleracea, 17 and Laminaria japonica.…”

Section: Effect Of Pgps On Semen Quality Parameters In Kkay Micementioning

confidence: 99%

“…1 More than 90% of diabetic patients are diagnosed as non-insulin-dependent (type 2 DM), which is characterized by hyperglycemia and dyslipidemia caused by the defects of either insulin secretion or action. [3][4][5][6][7][8] Talebi et al 6 reported that vitamin C attenuated the detrimental effects of DM on sperm parameters, chromatin quality, and rate of apoptosis in mice; Gonzales et al 7 found that the mixture of extracts from Peruvian plants (black maca and yacon) improved sperm count and reduced glycemia in mice with streptozotocininduced diabetes. [3][4][5][6][7][8] Talebi et al 6 reported that vitamin C attenuated the detrimental effects of DM on sperm parameters, chromatin quality, and rate of apoptosis in mice; Gonzales et al 7 found that the mixture of extracts from Peruvian plants (black maca and yacon) improved sperm count and reduced glycemia in mice with streptozotocininduced diabetes.…”

Section: Introductionmentioning

confidence: 99%

“…2 Many studies reported that DM impaired sperm parameters and decreased testosterone serum levels. [3][4][5][6][7][8] Talebi et al 6 reported that vitamin C attenuated the detrimental effects of DM on sperm parameters, chromatin quality, and rate of apoptosis in mice; Gonzales et al 7 found that the mixture of extracts from Peruvian plants (black maca and yacon) improved sperm count and reduced glycemia in mice with streptozotocininduced diabetes.…”

Section: Introductionmentioning

confidence: 99%

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