Vitamin C and glycohemoglobin

Abstract

Three groups of 10 age- and sex-matched nondiabetic volunteers took 0, 750, or 1500 mg of vitamin C each day for 12 weeks. Glycohemoglobin (GHb) was measured by HPLC, electrophoresis, affinity chromatography, and immunoassay at baseline (-4 weeks and -1 day), during supplementation (6 weeks and 12 weeks), and after supplementation ended (6 and 12 weeks). Plasma vitamin C increased twofold during supplementation but, in contrast with the results of Davie et al. (Diabetes 1992; 41:167-73), there were no between-group differences in GHb, glucose, and fructosamine concentrations. Fructosamine may have increased with storage time. The net effects of vitamin C on absolute GHb at 12 weeks vs -1 day (and at 12 weeks vs 12 weeks after) in % GHb amounted to: HPLC -0.035 (-0.050); electrophoresis +0.005 (+0.035); affinity chromatography -0.070 (+0.015); and immunoassay -0.110 (+0.025). We conclude that supplementation of nondiabetics with 750 or 1500 mg of vitamin C daily for 12 weeks does not cause interference in GHb determinations by HPLC, electrophoresis, affinity chromatography, or immunoassay, and does not reduce in vivo Hb glycation.

Capillarys 2 Flex Piercing: Analytical performance assessment according to CLSI protocols for HbA1c quantification

“…Concerning the interference of Hb variants, we reported no difference between both apparatus for HbA 1c quantification in case of the presence of S and C variants. Our data are consistent with those of previous studies [11,24,25]. In agreement with the observation made by Jaisson et al [11] an additional peak merging with HbA 0 appeared on the electropherogram.…”

“…In agreement with the observation made by Jaisson et al [11] an additional peak merging with HbA 0 appeared on the electropherogram. This would be probably due to the presence of glycated fraction of Hb C. More recently, in a brief technical report Weykamp et al [24] did not observe the presence of the shoulder in HbA 0 peak. However, both authors agreed that the presence of Hb C with its glycated fraction does not impair HbA 1c separation.…”

Evaluation of anti-diabetic and antioxidant properties of vitamin E and vitamin C in alloxan induced diabetic rats

“…Vitamin E found to reduce free oxygen species that commonly found to be increased in diabetes (Weykamp et al, 1995). The results for vitamin C (Ness et al, 1999) and Vitamin E (Jain and Levine, 1995) are agreed with other.…”

Hemorheological Failure in the Pathology of Cardio-Vascular Complications in Patients With Diabetic Foot Syndrome

“…The reflection of this process is the raise of malonic dialdehyde concentration, where it is a final product of repoxide oxidation of lipids at cyclooxigenase cascade of arachidonic acid in platelet membrane of patients with carbohydrate metabolism concentration [3,18,19]. The increase of glycogen quantity in cellular cytoplasm, the raise of cytoplasmatic Са+ that induces biosynthesis of thromboxane of blood platelets and raises the aggregation activity of platelets take place in platelets membrane of patients with DM as the result of metabolic disorders [20].…”