Vitamin B6 in Plasma and Cerebrospinal Fluid of Children

Abstract

BackgroundOver the past years, the essential role of vitamin B6 in brain development and functioning has been recognized and genetic metabolic disorders resulting in functional vitamin B6 deficiency have been identified. However, data on B6 vitamers in children are scarce.Materials and MethodsB6 vitamer concentrations in simultaneously sampled plasma and cerebrospinal fluid (CSF) of 70 children with intellectual disability were determined by ultra performance liquid chromatography-tandem mass spectrometry. For ethical reasons, CSF samples could not be obtained from healthy children. The influence of sex, age, epilepsy and treatment with anti-epileptic drugs, were investigated.ResultsThe B6 vitamer composition of plasma (pyridoxal phosphate (PLP) > pyridoxic acid > pyridoxal (PL)) differed from that of CSF (PL > PLP > pyridoxic acid > pyridoxamine). Strong correlations were found for B6 vitamers in and between plasma and CSF. Treatment with anti-epileptic drugs resulted in decreased concentrations of PL and PLP in CSF.ConclusionWe provide concentrations of all B6 vitamers in plasma and CSF of children with intellectual disability (±epilepsy), which can be used in the investigation of known and novel disorders associated with vitamin B6 metabolism as well as in monitoring of the biochemical effects of treatment with vitamin B6.

The value of plasma vitamin B6 profiles in early onset epileptic encephalopathies

“…Some of the anticonvulsants seem to inhibit pyridoxal kinase (di Salvo et al 2012). Nevertheless, our results are in line with data by Albersen et al who showed that vitamin B 6 vitamer concentrations in plasma were not influenced by epilepsy nor by anticonvulsants (Albersen et al 2015).…”

Should we give vitamins to children?

“…У грудных детей дефицит витамина В6 может проявляется повы-шенной возбудимостью ЦНС, периодически судорогами, что связано с недостаточным образованием тормозного медиатора ГАМК. Доказана роль пиридоксина во внутри-утробном и постнатальном развити головного мозга [49]. Дефицит витамина В6 ассоциирован с повышенным риском развития рака легких [50], ишемического инсуль-та, сахарного диабета [51], депрессивными состояниями [52].…”

Epilepsy as a Pyridoxine-Dependent Condition: Quantitative Urinary Biomarkers of Epilepsy. Family Disorders of Pyridoxine Metabolism

“…Two non-sanguineous families were studied. Samples of each person were repeatedly analyzed over several (2)(3)(4)(5)(6)(7)(8) consecutive days. The mean value of each parameter is presented in Table 2.…”

“…Vitamin B6 consists of six different vitamers: pyridoxine (PN), pyridoxal (PL), pyridoxamine (PM), and their phosphate-esterified forms. Pyridoxal phosphate (PLP) is the active B6 vitamer, which is produced from its precursor vitamers (PN, PM, and PL) by phosphorylation and oxidation (PN phosphate, PLP, and PM phosphate) through the actions of pyridoxal kinase and pyridox(am)ine oxidase (PNPO), respectively (Figure 1) [6]. Vitamin B6 is broken down to pyridoxic acid (4PA), which is excreted in the urine.…”