Vitamin B12b increases the cytotoxicity of short-time exposure to ascorbic acid, inducing oxidative burst and iron-dependent DNA damage

Solovieva, Marina E.; Soloviev, Valery V.; Акатов, В. С.

doi:10.1016/j.ejphar.2007.03.035

Cited by 23 publications

(16 citation statements)

References 24 publications

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“…Since the structures and uptake systems of heme and cobalamin are alike (5), and the known cytotoxic and antitumor activity of cobalamins administered either alone or combined with antioxidants are also similar (10,18,19,23), it was of great interest to investigate the effect of cyanocobalamin (vitamin B 12 ) on T. cruzi.…”

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confidence: 99%

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Ciccarelli

Frank

Puente

et al. 2012

Antimicrob Agents Chemother

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A nutritional characteristic of trypanosomatid protozoa is that they need a heme compound as a growth factor. Because of the cytotoxic activity of heme and its structural similarity to cobalamins, we have investigated the in vitro and in vivo effect of vitamin B 12 (or cyanocobalamin) on the different forms of Trypanosoma cruzi. Cyanocobalamin showed a marked antiparasitic activity against epimastigotes (50% inhibitory concentration [IC 50 ], 2.42 M), amastigotes (IC 50 , 10.69 M), and trypomastigotes (IC 50 , 9.46 M). Anti-epimastigote and -trypomastigote values were 1.7 to 4 times lower than those obtained with the reference drug benznidazole (Bnz). We also found that B 12 and hemin do not interact with each other in their modes of action. Our results show that B 12 increases intracellular oxidative activity and stimulates both superoxide dismutase (50%) and ascorbate peroxidase (20%) activities, while the activity of trypanothione reductase was not modified. In addition, we found that the antioxidants dithiothreitol and ascorbic acid increase the susceptibility of the parasite to the cytotoxic action of B 12 . We propose that vitamin B 12 exerts its growth-inhibitory effect through the generation of reactive oxygen species. In an in vivo assay, a significant reduction in the number of circulating parasites was found in T. cruzi-infected mice treated with cyanocobalamin and ascorbic acid. The reduction of parasitemia in benznidazole-treated mice was improved by the addition of these vitamins. According to our results, a combination of B 12 and Bnz should be further investigated due to its potential as a new therapeutic modality for the treatment of Chagas' disease.

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confidence: 99%

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Ciccarelli

Frank

Puente

et al. 2012

Antimicrob Agents Chemother

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“…Indeed, an excess of these antioxidants elevates the incidence of cancer (Nitta et al 1991;Omenn et al, 1996). Solovieva et al reported that antioxidants, ascorbic acid (Solovieva et al, 2007) and dithiothreitol (Solovieva et al, 2008), exhibit cytotoxicity via H 2 O 2 generation. Relevantly, it has been reported that vitamins A (Murata & Kawanishi, 2000) and E (Yamashita et al, 1998) and catechins (Oikawa et al, 2003) induce DNA oxidation through H 2 O 2 generation during their oxidation.…”

Section: Catalytic Decomposition Of Hydrogen Peroxide By Metal Nanopamentioning

confidence: 99%

Self-Organization of Silver-Core Bimetallic Nanoparticles and Their Application for Catalytic Reaction

Hirakawa¹

2012

Smart Nanoparticles Technology

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“…Besides, vitamin C may exert prooxidant or antioxidant effects in a dosedependent manner 9,10 , and its cytotoxicity may be increased by concomitant use of vitamin B12 11 . In this way, vitamin C might either reduce or enhance the oxidative burst when simultaneously administered with benznidazole, and therefore concerns exist regarding this treatment's effi cacy and safety.…”

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Authors' reply: Vitamins in the treatment of chronic Chagas disease: adjuvant antiparasitary or antioxidant therapy?

Andrade

Oliveira

Nagão-Dias

et al. 2014

Rev. Soc. Bras. Med. Trop.

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Vitamin B12b increases the cytotoxicity of short-time exposure to ascorbic acid, inducing oxidative burst and iron-dependent DNA damage

Cited by 23 publications

References 24 publications

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Self-Organization of Silver-Core Bimetallic Nanoparticles and Their Application for Catalytic Reaction

Authors' reply: Vitamins in the treatment of chronic Chagas disease: adjuvant antiparasitary or antioxidant therapy?

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Vitamin B12b increases the cytotoxicity of short-time exposure to ascorbic acid, inducing oxidative burst and iron-dependent DNA damage

Cited by 23 publications

References 24 publications

Antiparasitic Effect of Vitamin B 12 on Trypanosoma cruzi

Antiparasitic Effect of Vitamin B 12 on Trypanosoma cruzi

Self-Organization of Silver-Core Bimetallic Nanoparticles and Their Application for Catalytic Reaction

Authors' reply: Vitamins in the treatment of chronic Chagas disease: adjuvant antiparasitary or antioxidant therapy?

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Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi