Vitamin B12b enhances the cytotoxicity of dithiothreitol

Solovieva, Marina E.; Solovyev, V. V.; Kudryavtsev, A. A.; Trizna, Yuliya A.; Акатов, В. С.

doi:10.1016/j.freeradbiomed.2008.02.002

Cited by 32 publications

(37 citation statements)

References 54 publications

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“…By adding antioxidants such as DTT or vitamin C, the oxidative damage increases instead of decreases, probably due to the wellknown prooxidant effect of these compounds combined with transition metal ions (Fe, Cu, and Co), which makes itself evident through the generation of ROS (18,19). Concerning the possible mechanism of action of B 12 , additional studies should carried out.…”

Section: Discussionmentioning

confidence: 99%

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Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Ciccarelli

Frank

Puente

et al. 2012

Antimicrob Agents Chemother

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A nutritional characteristic of trypanosomatid protozoa is that they need a heme compound as a growth factor. Because of the cytotoxic activity of heme and its structural similarity to cobalamins, we have investigated the in vitro and in vivo effect of vitamin B 12 (or cyanocobalamin) on the different forms of Trypanosoma cruzi. Cyanocobalamin showed a marked antiparasitic activity against epimastigotes (50% inhibitory concentration [IC 50 ], 2.42 M), amastigotes (IC 50 , 10.69 M), and trypomastigotes (IC 50 , 9.46 M). Anti-epimastigote and -trypomastigote values were 1.7 to 4 times lower than those obtained with the reference drug benznidazole (Bnz). We also found that B 12 and hemin do not interact with each other in their modes of action. Our results show that B 12 increases intracellular oxidative activity and stimulates both superoxide dismutase (50%) and ascorbate peroxidase (20%) activities, while the activity of trypanothione reductase was not modified. In addition, we found that the antioxidants dithiothreitol and ascorbic acid increase the susceptibility of the parasite to the cytotoxic action of B 12 . We propose that vitamin B 12 exerts its growth-inhibitory effect through the generation of reactive oxygen species. In an in vivo assay, a significant reduction in the number of circulating parasites was found in T. cruzi-infected mice treated with cyanocobalamin and ascorbic acid. The reduction of parasitemia in benznidazole-treated mice was improved by the addition of these vitamins. According to our results, a combination of B 12 and Bnz should be further investigated due to its potential as a new therapeutic modality for the treatment of Chagas' disease.

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Section: Discussionmentioning

confidence: 99%

“…Since the structures and uptake systems of heme and cobalamin are alike (5), and the known cytotoxic and antitumor activity of cobalamins administered either alone or combined with antioxidants are also similar (10,18,19,23), it was of great interest to investigate the effect of cyanocobalamin (vitamin B 12 ) on T. cruzi.…”

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confidence: 99%

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Ciccarelli

Frank

Puente

et al. 2012

Antimicrob Agents Chemother

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“…Antibiotics gentomicin (Asparin, Germany) and amphotolecin B (Sigma) used for prevention of microbial contamination at the cell culture (Solovieva et al, 2008) were dissolved in 2 ml of D 2 O to ensure final concentrations of 40 µg/ml (gentamicin) and 2 µg/ml (amphotolecin B) in the mitochondria suspension samples and of 5 µg/ml (gentamicin) and 0.4 µg/ml (amphotolecin B) in the liver homogenate samples. Sodium azide concentration was kept at the level of 0.2%.…”

Section: Methodsmentioning

confidence: 99%

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

Kutyshenko

Beskaravayny

Молчанов

et al. 2013

PeerJ

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We analyzed the applicability of high-resolution 2H-HMR spectroscopy for the analysis of microbe metabolism in samples of mitochondrion isolated from rat liver and from aqueous extracts of homogenates of rat liver and other organs and tissues in the presence of high D2O contents. Such analysis is possible due to the fast microbe adaptation to life in the heavy water. It is also shown that some enzymatic processes typical for the intact cells are preserved in the homogenized tissue preparations. The microbial and cellular metabolic processes can be differentiated via the strategic use of cell poisons and antibiotics.

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“…Plates were incubated at 37 o C and read 24 hours later. In part of the experiments, combination of ciprofloxacin at 100µg/mL and vorinostat at 100µM were mixed with the agar [21][22][23]. The zones of growth inhibition surrounding the antibiotic containing discs were measured.…”

Section: Testing Of Antimicrobial Susceptibilitymentioning

confidence: 99%

Vorinostat Pretreatment Enhanced Ciprofloxacin-Induced Antibacterial Activity

Masadeh

Alzoubi

Al‐Azzam

et al. 2016

Preprint

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Abstract:The mechanism of ciprofloxacin action involves interference with transcription and replication of bacterial DNA, which results in elevated oxidative stress, and bacterial cell death.Vorinostat was shown to induce oxidative DNA damage. In the current work, the possibility for bacterial strains compared ciprofloxacin alone. As a conclusion, current results showed the possible agonistic properties for vorinostat when it is used together with ciprofloxacin. Future research will be focus on molecular mechanisms possible for such interactive effect.

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Vitamin B12b enhances the cytotoxicity of dithiothreitol

Cited by 32 publications

References 54 publications

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy

Vorinostat Pretreatment Enhanced Ciprofloxacin-Induced Antibacterial Activity

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Vitamin B12b enhances the cytotoxicity of dithiothreitol

Cited by 32 publications

References 54 publications

Antiparasitic Effect of Vitamin B 12 on Trypanosoma cruzi

Antiparasitic Effect of Vitamin B 12 on Trypanosoma cruzi

Looking at microbial metabolism by high-resolution2H-NMR spectroscopy

Vorinostat Pretreatment Enhanced Ciprofloxacin-Induced Antibacterial Activity

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Product

Resources

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Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Antiparasitic Effect of Vitamin B ₁₂ on Trypanosoma cruzi

Looking at microbial metabolism by high-resolution²H-NMR spectroscopy